Role of Pycnoporus coccineus laccase in the degradation of aromatic compounds in olive oil mill wastewater

Jaouani, Atef; Guillén, Francisco; Penninckx, Michel; Martı́nez, Ángel T.; Martı́nez, Marı́a Jesús

doi:10.1016/j.enzmictec.2004.11.011

Cited by 128 publications

(62 citation statements)

References 48 publications

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“…Relative activities at pH 4, 5, 6, and 7 were 71.8 ± 1.7, 62.4 ± 1.5, 54.8 ± 1.8, and 0.43 ± 0.04%, respectively. A similar pH optimum was previously reported for the purified laccase from P. coccineus MUCL38527 22 . The enzyme seemed to be relatively stable up to pH 8, with around 75% and 40% activity remaining after 1-h incubation at pH 8 and 9, respectively, (Fig.…”

Section: Fungal Collection and Screening For Laccase Producersupporting

confidence: 66%

“…Both P. coccineus isolates produced higher laccase activity (3.31-3.61 U/ml) than that of P. sanguineus Thongkred 015 BCU (2.65 U/ml) and the other previously reported Thai isolates (0.46-2.53 U/ml) 11 . A broadly similar level of extracellular laccase activity has also been reported in some P. coccineus strains 22,23 . Manganese peroxidase activity was also detected in the culture filtrate of all three isolates, ranging from 0.03 U/ml in P. sanguineus Thongkred 015 to 0.13 U/ml in P. coccineus Thongkred 014 BCU (Table 1).…”

Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 49%

“…No lignin peroxidase was detected in any of the isolates (data not shown). It was also reported that P. coccineus did not produce any detectable lignin peroxidase even though a lignin peroxidase-like DNA sequence has been annotated in the genome 22,24 . As the best laccase producer among the three Pycnoporus isolates, P. coccineus Thongkred 013 BCU was selected for the optimization of extracellular laccase production.…”

Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 99%

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Thongkred¹,

Lotrakul²,

Prasongsuk³

et al. 2011

ScienceAsia

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Basidiocarps of Pycnoporus coccineus and P. sanguineus were collected from Kanchanaburi, Phetchaburi, and Mae Hong Son provinces in Thailand. Liquid culture of P. coccineus isolates provided higher laccase and manganese peroxidase activities than those of P. sanguineus. Lignin peroxidase activity was not detectable in any of the three Pycnoporus isolates. P. coccineus Thongkred 013 BCU, the best laccase producer among these isolates, was selected to optimize laccase production. The highest laccase yield (5.97 ± 0.40 U/ml) was obtained when grown at 28°C for 8 days in basal medium containing 2% (w/v) glucose and 0.25% (w/v) peptone at an initial pH of 5.0. The optimum conditions for stability and activity of the crude laccase enzyme were at pH 3.5 and room temperature (25 ± 2°C). FeSO4 (10 mM) and SDS (1% w/v) were both inhibitory to the laccase activity. When five different types of polycyclic aromatic hydrocarbons (PAHs) were individually added to liquid culture of P. coccineus at 100 ppm, the oxidation of anthracene, pyrene, and fluoranthene detected within 24 h were 59.7, 50.7, and 49.8%, respectively, whereas only 25.3% of benzo[a]pyrene and 32.4% of phenanthrene were oxidized. When P. coccineus crude laccase (1 U/ml) was used, these PAHs were more readily oxidized, with 76.4 and 74.3% of pyrene and anthracene, respectively, being oxidized within 2 h and 84.2% of fluoranthene oxidized within 24 h. Benzo[a]pyrene and phenanthrene oxidation by the enzyme were enhanced when 1 mM 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) was added as a mediator to the reaction mixture, resulting in 66.3 and 50.5% of the initial 100 ppm being oxidized after 24 h.

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Section: Fungal Collection and Screening For Laccase Producersupporting

confidence: 66%

Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 49%

Section: Fungal Collection and Screening For Laccase Producermentioning

confidence: 99%

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Untitled

Thongkred¹,

Lotrakul²,

Prasongsuk³

et al. 2011

ScienceAsia

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“…Awad and Karume [26] reported that occurrence of fungi in aerobic and anoxic activated sludge from MBRs (membrane bioreactors). Under aerobic condition, the Geotrichum was found at (8.8%) followed by Penicillium (75.0%), Yeasts (65.7%) and Trichoderma (55.5%), while Yeasts (77.1%) Geotrichum candidum and Penicillium (61.1%) species were the most prevalent in anoxic activated sludge.…”

Section: Discussionmentioning

confidence: 99%

The Role of Formed Microorganism in Sludge on Processing of Wastewater Treatment

Al-Hussieny¹,

Obeid²,

Baqer³

et al. 2015

JLS

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Several microorganisms such as bacteria, fungi, Protozoa, Rotifera, cystic amoeba and algae diagnosed in activated sludge aerobic (Rustumiya treatment plant) and anaerobic reactor. Results have shown a reduction in the turbidity rates when using activated sludge at Rustumiya plant of 76.3 to 2.653 NTU in pre-treatment and final tank respectively, also COD (chemical oxygen demand) amount reduced from 427.263 to 82 mg/L respectively. In addition, concentrations of phosphates and nitrates decreased from 12.083 to 8.426 mg/L and 3.59 to 2.43 mg/L respectively, by removing 30.2% and 32.3% respectively of the final tank. The ratio of ammonia removing was 89.6% for ammonia, reducing process from 1358 to 140 mg/L. Furthermore, sulfates concentration decreased from 30.883 to 23.337 mg/L. However, the system in the anaerobic reactor depends on non-aerated activated sludge. Results show turbidity reduced from 12.5 to 2 NTU in pre-treatment and final tank respectively, also the COD mount reduced from 191 to 130 mg/L, the percentage removal of 31.9%. In addition phosphates, nitrates and sulfates concentrations were decreased by using activated sludge from 17.15 to 8.15, 1.2 to 0.1 and 28 to 9.2 mg/L respectively. The ammonia concentration has reduced from 1.2 to 0.1 mg/L where at a removal percentage of 90.9%.

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“…The highest laccase level was 100 000 U/l after 45 incubation-days. The enzyme was stable at pH 7, at room temperature and showed a half-life of 8 and 2 h at 50 and 60°C, respectively (Jaouani et al 2005). In order to decolourise OMWW efficiently, production and differential induction of ligninolytic enzymes by the white rot Coriolopsis polyzona, were studied by varying growth media composition and/or inducer addition (Jaouani et al 2006).…”

Section: Enzymesmentioning

confidence: 99%

Microbial Biotechnology in Olive Oil Industry

Darvishi¹

2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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Role of Pycnoporus coccineus laccase in the degradation of aromatic compounds in olive oil mill wastewater

Cited by 128 publications

References 48 publications

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The Role of Formed Microorganism in Sludge on Processing of Wastewater Treatment

Microbial Biotechnology in Olive Oil Industry

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