Role of Putrescine on Androgen-Elicited Positive Inotropism in the Left Atrium of Rats

Velasco, Lucía; Secades, Lorena; Bordallo, Carmen; Bordallo, Javier; Boto, María José García de; Rubín, José M.; Hidalgo, Agustı́n; Cantabrana, Begoña; Sánchez, Manuel

doi:10.1097/fjc.0b013e31818018aa

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…The amines were determined using a pre-column derivatisation method, as previously described [16,33]. Laboratory chow and intestinal content samples were homogenised in 0.5 mL purified water and then treated with sufficient perchloric acid to reach a final concentration of 12% (5 min at 4°C).…”

Section: Methodsmentioning

confidence: 99%

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Sánchez

Suárez

Andrés

et al. 2017

Food & Nutrition Research

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Background: Gastrointestinal motility modulatory factors include substances of the intestinal content, such as polyamines and trace amines (TAs), the focus of this study.Methods: The amines of food, intestinal content and from faecal bacteria of Swiss mice were determined by HPLC and functionally characterised in isolated distal ileum and medial colon rings.Results: Mouse food and intestinal content contain polyamines (spermidine>putrescine>spermine) and TAs (isoamylamine>cadaverine). Intestinal bacteria mainly produce putrescine and cadaverine. The amines inhibited the spontaneous motility of the ileum (0.1-3 mM) and colon rings (0.01-3 mM, with lower IC50), with: spermine~isoamylamine~spermidine. Spermine inhibition was tetrodotoxin (TTX)-insensitive, while isoamylamine was TTX-sensitive, suggesting neural control. Mainly in the ileum, isoamylamine (3 mM) elicited acute effects modified by TTX, atropine and propranolol, and suppressed by spermine (3 mM), not being localized at the smooth muscle level. The amines assayed (3 mM), except putrescine and cadaverine in the ileum and isoamylamine in the colon, antagonised acetylcholine (ACh, 0.1 mM)-elicited phasic contractions. Isoamylamine and spermine in colon relaxed KCl (100 mM)-elicited tonic contractions, suggesting an effect on smooth muscle, but did not justify the suppression of motility caused by spermine and isoamylamine.Conclusions: Polyamines and TAs of the intestinal content might act on chemosensors and modulate intestinal peristalsis.

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Section: Methodsmentioning

confidence: 99%

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Sánchez

Suárez

Andrés

et al. 2017

Food & Nutrition Research

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“…Polyamine levels and trace amines were determined using a precolumn derivatization method as previously described , using dansyl chloride. Quantification of polyamines was performed with 2‐hydroxydiaminopropane as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

“…Exogenous polyamines produce different effects, depending on the compound assayed: Spermine and spermidine elicit negative inotropism , while putrescine produces a cardiotonic response . These results might be explained by the fact that polyamines bind with different affinities to a variety of cytoplasmic ligands and many membrane proteins, including several types of ion channels and β‐adrenoceptors, causing an increase in intracellular cAMP .…”

Section: Introductionmentioning

confidence: 99%

Correlation between endogenous polyamines in human cardiac tissues and clinical parameters in patients with heart failure

Meana

Rubín²,

Bordallo

et al. 2015

J Cellular Molecular Medi

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Polyamines contribute to several physiological and pathological processes, including cardiac hypertrophy in experimental animals. This involves an increase in ornithine decarboxylase (ODC) activity and intracellular polyamines associated with cyclic adenosine monophosphate (cAMP) increases. The aim of the study was to establish the role of these in the human heart in living patients. For this, polyamines (by high performance liquid chromatography) and the activity of ODC and N1‐acetylpolyamine oxidases (APAO) were determined in the right atrial appendage of 17 patients undergoing extracorporeal circulation to correlate with clinical parameters. There existed enzymatic activity associated with the homeostasis of polyamines. Left atria size was positively associated with ODC (r = 0.661, P = 0.027) and negatively with APAO‐N1‐acetylspermine (r = −0.769, P = 0.026), suggesting that increased levels of polyamines are associated with left atrial hemodynamic overload. Left ventricular ejection fraction (LVEF) and heart rate were positively associated with spermidine (r = 0.690, P = 0.003; r = 0.590, P = 0.021) and negatively with N1‐acetylspermidine (r = −0.554, P = 0.032; r = −0.644, P = 0.018). LVEF was negatively correlated with cAMP levels (r = −0.835, P = 0.001) and with cAMP/ODC (r = −0.794, P = 0.011), cAMP/spermidine (r = −0.813, P = 0.001) and cAMP/spermine (r = −0.747, P = 0.003) ratios. Abnormal LVEF patients showed decreased ODC activity and spermidine, and increased N1‐acetylspermidine, and cAMP. Spermine decreased in congestive heart failure patients. The trace amine isoamylamine negatively correlated with septal wall thickness (r = −0.634, P = 0.008) and was increased in cardiac heart failure. The results indicated that modifications in polyamine homeostasis might be associated with cardiac function and remodelling. Increased cAMP might have a deleterious effect on function. Further studies should confirm these findings and the involvement of polyamines in different stages of heart failure.

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“…The amines were determined using a pre-column derivatisation method, as previously described (47,48) . The samples of faeces were homogenised and then centrifuged at 4000 rpm for 3 min at room temperature to obtain 300 μl supernatant.…”

Section: Determination Of Amines In the Different Types Of Milk And Nmentioning

confidence: 99%

Amine variations in faecal content in the first weeks of life of newborns in relation to breast-feeding or infant formulas

et al. 2019

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Breast-feeding is the ideal nutrition for a newborn’s integral necessities. It seems crucial therefore to know its composition in order to provide suitable infant formula when required. Of these, polyamines (with lactation and the microbiota being its intestinal source) are involved in the development of gut epithelium and immunity. Safety concerns limit human intervention studies. Therefore, we studied the amounts of polyamines supplied by breast milk (varying among mothers) or infant formula feeding, up to day 30 postpartum, in the faeces of newborns. Independent samples (68) of breast milk from fifty-nine healthy Caucasian woman (day 0, 1, 3, 5, 7, 15 and 30 postpartum) who had natural deliveries after week 38, same-day faeces of newborns when available (eighty-one from breast milk and fifty-five from infant formula fed) and six infant formulas were collected and the polyamine content was determined by HPLC. In breast milk, polyamines and isoamylamine (a primary amine), with inter-individual variations, increased over time (with a higher content of spermidine; no other amines were present). Overall, they were much higher than in infant formula. By the 2nd week after birth, polyamines, cadaverine and tyramine, but not isoamylamine, were higher in the faeces of those fed infant formula compared with those fed breast milk. Cadaverine and tyramine could be used to predict the feeding type used for newborns. The differences in the content may be related to distinct colonisation of amine-producing bacteria, which can be established by polyamines. Further studies are required to determine the clinical utility of these findings.

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Role of Putrescine on Androgen-Elicited Positive Inotropism in the Left Atrium of Rats

Cited by 10 publications

References 33 publications

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Modulatory effect of intestinal polyamines and trace amines on the spontaneous phasic contractions of the isolated ileum and colon rings of mice

Correlation between endogenous polyamines in human cardiac tissues and clinical parameters in patients with heart failure

Amine variations in faecal content in the first weeks of life of newborns in relation to breast-feeding or infant formulas

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