Role of PUM RNA-Binding Proteins in Cancer

Smialek, Maciej Jerzy; Ilaslan, Erkut; Sajek, Marcin

doi:10.3390/cancers13010129

Cited by 14 publications

(12 citation statements)

References 67 publications

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“…Recently, it was reported that mouse Dnd1 is a critical partner of both Nanos2 and Nanos3 proteins in increasing the susceptibility of teratoma formation of PGCs [ 55 ]. Moreover, PUM proteins, one of the best examples of NANOS partner RBPs, were shown to be overexpressed in many types of cancer [ 56 ]. It is likely that the NANOS interactome extends beyond DND1, PUM proteins, and CCR4-NOT deadenylase complexes.…”

Section: Future Perspectivesmentioning

confidence: 99%

Emerging Roles of NANOS RNA-Binding Proteins in Cancer

Ilaslan

Sajek

Jaruzelska

et al. 2022

IJMS

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In recent years, growing evidence demonstrates that mammalian Nanos RNA-binding proteins (Nanos1, Nanos2, and Nanos3), known for their indispensable roles in germline development, are overexpressed in a variety of cancers. This overexpression contributes to various oncogenic properties including cancer growth, invasiveness, and metastasis. Here, we highlight recent findings regarding the role of mammalian Nanos RNA-binding proteins and the mechanisms of their overexpression in cancer. In addition, we present expression profiles of human NANOS genes and their oncogenic transcriptional regulators obtained from publicly available cancer and normal tissue RNA-Seq datasets. Altogether, we emphasize the functional significance of NANOS proteins across human cancers as well as highlight the missing links to understanding the full scope of their role in carcinogenesis.

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Section: Future Perspectivesmentioning

confidence: 99%

Emerging Roles of NANOS RNA-Binding Proteins in Cancer

Ilaslan

Sajek

Jaruzelska

et al. 2022

IJMS

Self Cite

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“…Additional evidence for post-transcriptional regulatory roles of M17 comes from the analysis of RBP binding sites identified from recently available eCLIP data ( 47 , 48 ) (Figure 2 ). Notable RBPs include the translational repressor and cell proliferation regulator PUM2 and the translational and microRNA-mediated degradation regulators IGF2BP1-3; which could suggest that M17 is involved in regulation of gene expression, cell viability, and localization ( 63–65 ) via modulation of interactions with these regulatory RBPs. Interestingly, four m6A modifications also overlap the RBP interaction sites on M17 (Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

Prediction and analysis of functional RNA structures within the integrative genomics viewer

Rouse¹,

Andrews²,

Booher³

et al. 2022

NAR Genomics and Bioinformatics

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In recent years, interest in RNA secondary structure has exploded due to its implications in almost all biological functions and its newly appreciated capacity as a therapeutic agent/target. This surge of interest has driven the development and adaptation of many computational and biochemical methods to discover novel, functional structures across the genome/transcriptome. To further enhance efforts to study RNA secondary structure, we have integrated the functional secondary structure prediction tool ScanFold, into IGV. This allows users to directly perform structure predictions and visualize results—in conjunction with probing data and other annotations—in one program. We illustrate the utility of this new tool by mapping the secondary structural landscape of the human MYC precursor mRNA. We leverage the power of vast ‘omics’ resources by comparing individually predicted structures with published data including: biochemical structure probing, RNA binding proteins, microRNA binding sites, RNA modifications, single nucleotide polymorphisms, and others that allow functional inferences to be made and aid in the discovery of potential drug targets. This new tool offers the RNA community an easy to use tool to find, analyze, and characterize RNA secondary structures in the context of all available data, in order to find those worthy of further analyses.

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“…According to the differential correlation analysis of publicly available testis and testicular cancer expression datasets, the NANOS3–PUM1–FOXM1 axis is dysregulated in testis cancer. Previously it was shown that an abnormal expression of NANOS and PUM genes is observed in many types of cancer [ 56 , 57 , 58 , 59 ]. FOXM1 is a critical driver of proliferation and it is overexpressed in a broad range of cancers.…”

Section: Discussionmentioning

confidence: 99%

Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

Ilaslan

Kwiatkowska

Smialek

et al. 2022

IJMS

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Nanos RNA-binding proteins are critical factors of germline development throughout the animal kingdom and their dysfunction causes infertility. During evolution, mammalian Nanos paralogues adopted divergent roles in germ cell biology. However, the molecular basis behind this divergence, such as their target mRNAs, remains poorly understood. Our RNA-sequencing analysis in a human primordial germ cell model‑TCam-2 cell line revealed distinct pools of genes involved in the cell cycle process downregulated upon NANOS1 and NANOS3 overexpression. We show that NANOS1 and NANOS3 proteins influence different stages of the cell cycle. Namely, NANOS1 is involved in the G1/S and NANOS3 in the G2/M phase transition. Many of their cell cycle targets are known infertility and cancer-germ cell genes. Moreover, NANOS3 in complex with RNA-binding protein PUM1 causes 3′UTR-mediated repression of FOXM1 mRNA encoding a transcription factor crucial for G2/M phase transition. Interestingly, while NANOS3 and PUM1 act as post-transcriptional repressors of FOXM1, FOXM1 potentially acts as a transcriptional activator of NANOS3, PUM1, and itself. Finally, by utilizing publicly available RNA-sequencing datasets, we show that the balance between FOXM1-NANOS3 and FOXM1-PUM1 expression levels is disrupted in testis cancer, suggesting a potential role in this disease.

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Role of PUM RNA-Binding Proteins in Cancer

Cited by 14 publications

References 67 publications

Emerging Roles of NANOS RNA-Binding Proteins in Cancer

Emerging Roles of NANOS RNA-Binding Proteins in Cancer

Prediction and analysis of functional RNA structures within the integrative genomics viewer

Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

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