Role of protein kinase a in LPS-induced activation of NF-κB proteins of a mouse macrophage-like cell line, J774

Protein kinase A mediates microglial activation induced by plasminogen and gangliosides

“…Hiscott et al, 1993;Xie et al, 1993;Chang et al, 2004). NF-κB activation may be regulated by PKA since in J774 macrophage-like cells, H89 inhibited LPS-induced NF-κB activation (Muroi and Suzuki, 1993). The results presented in the current study show that PKA inhibitors reduced NF-κB DNA binding activity in plasminogen-treated microglia ( Figure 2B).…”

Emerging Roles of Prostaglandins in HIV-1 Transcription

“…Results from mobility shift assays suggest that the PGE 2 -mediated effect on HIV-1 LTR activity is due to activation of the transcription factor NF-B. This is in agreement with the previous demonstration that PGE 2 activates NF-B in the macrophage-like cell line J774 (Muroi & Suzuki, 1993). The fact that we have noticed that both NF-B and Ca 2+ are key elements in the PGE 2 effect on HIV-1 transcription is of interest considering that calcineurin, a Ca 2+ /calmodulin-dependent serine/threonine protein phosphatase, has been reported to activate NFB through the inactivation of IB (Frantz et al, 1994).…”

Lipopolysaccharide and Raf-1 Kinase Regulate Secretory Interleukin-1 Receptor Antagonist Gene Expression by Mutually Antagonistic Mechanisms

“…In the cytoplasm, it is possible that LPS activates PKA, which in turn antagonizes Raf-1 by phosphorylation of its kinase domain and down-regulation of its activity (32). Increases in cyclic AMP (cAMP) and PKA activity by LPS have been reported, and a role for cAMP in LPS regulation of IL-1␤ has been described (13,25,49). In our system, a role for the cAMP/PKA pathway in LPS signal transduction has yet to be tested.…”

“…How mutual antagonism for sIL-1Ra gene expression occurs between multiple activators of MAPK is not clear, but it is possible that MAPK activation is irrelevant to regulation of sIL-1Ra gene expression by LPS, Raf-1, and MEKK. The ability of LPS, Raf-1, and MEKK to regulate sIL-1Ra gene expression may be governed by their abilities to interact with other MAPK-independent signaling components (14,16,17,27,43,44,47,49,65). Alternatively, it is also possible that MAPK is important for regulation of sIL-1Ra gene expression by LPS, Raf-1, and MEKK, and the differences in the abilities of these components to regulate sIL-1Ra gene expression may be explained by differences in their abilities to regulate the duration of MAPK activation or the association of MAPK with different signaling complexes.…”