Role of Protein in Fungal Biomineralization of Copper Carbonate Nanoparticles

Liu, Feixue; Shah, Dinesh S.; Gadd, Geoffrey M.

doi:10.1016/j.cub.2020.10.044

Cited by 27 publications

(15 citation statements)

References 49 publications

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“…For the TEM analysis, about 10 mg nanoparticles was suspended in 1 ml Milli-Q water and 2 µl of the suspension solution was placed on ‘holey’ carbon-coated nickel grids (Agar Scientific, Essex, UK), and air dried prior to TEM analysis. For the confocal microscope analysis, the CuNPs sample was stained for 30 min at room temperature using 70 µM Fura-2AM (Abcam, UK) in the dark to visualize Cu, followed by staining using FilmTracer Sypro (Thermal Fisher, USA) to visualize proteins [ 32 ]. Stained samples were washed twice and resuspended using Milli-Q water, and 3 µl of resuspended samples were encased between a glass slide and a coverslip and sealed using nail varnish.…”

Section: Methodsmentioning

confidence: 99%

“…After 3 days of growth in complete AP1 medium, fungal biomass was collected and washed twice in sterile Milli-Q water by centrifugation (×4000 g, 30 min), and then incubated in sterile phosphate-free AP1 medium for 12 days at 125 r.p.m., 25 °C in the dark. CuNPs were produced following previous protocols [31,32]. Briefly, biomass-free N. crassa spent culture media was collected by centrifugation (×4000 g, 30 min) after 12 days of growth, filtered through a 0.2 µm pore-size cellulose acetate membrane filter (Sartorius Stedim Biotech, Göttingen, Germany) and mixed with CuCl 2 solution at a 20 mM final concentration.…”

Section: Biomineralization Of Cunpsmentioning

confidence: 99%

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Application of fungal copper carbonate nanoparticles as environmental catalysts: organic dye degradation and chromate removal

et al. 2021

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Biomineralization is a ubiquitous process in organisms to produce biominerals, and a wide range of metallic nanoscale minerals can be produced as a consequence of the interactions of micro-organisms with metals and minerals. Copper-bearing nanoparticles produced by biomineralization mechanisms have a variety of applications due to their remarkable catalytic efficiency, antibacterial properties and low production cost. In this study, we demonstrate the biotechnological potential of copper carbonate nanoparticles (CuNPs) synthesized using a carbonate-enriched biomass-free ureolytic fungal spent culture supernatant. The efficiency of the CuNPs in pollutant remediation was investigated using a dye (methyl red) and a toxic metal oxyanion, chromate Cr(VI). The biogenic CuNPs exhibited excellent catalytic properties in a Fenton-like reaction to degrade methyl red, and efficiently removed Cr(VI) from solution due to both adsorption and reduction of Cr(VI). X-ray photoelectron spectroscopy (XPS) identified the oxidation of reducing Cu species of the CuNPs during the reaction with Cr(VI). This work shows that urease-positive fungi can play an important role not only in the biorecovery of metals through the production of insoluble nanoscale carbonates, but also provides novel and simple strategies for the preparation of sustainable nanomineral products with catalytic properties applicable to the bioremediation of organic and metallic pollutants, solely and in mixtures.

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Section: Methodsmentioning

confidence: 99%

Section: Biomineralization Of Cunpsmentioning

confidence: 99%

Application of fungal copper carbonate nanoparticles as environmental catalysts: organic dye degradation and chromate removal

et al. 2021

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“…Geoactive fungi play an important role in the biomineralization of a broad range of minerals and have been investigated for their potential in the recycling of elements and biosynthesis of new biomaterials (Gadd 2007 ; Liang and Gadd 2017 ; Kang et al 2020 , 2021 ). The fundamental mechanisms behind fungal biomineralization involve the production of metabolites such as oxalate and carbon dioxide, or the release of ligands like carbonates and phosphates from organic and inorganic sources, as well as proteins and enzymes that can directly interact with the metal/mineral (Gadd 2007 , 2010 ; Li et al 2014 ; Kumari et al 2016 ; Dhami et al 2017 ; Fomina et al 2007 ; Liu et al 2019 ; Kirtzel et al 2020 ; Kang et al 2020 , 2021 ; Mendes et al 2021a , b ; Liu et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Solubilization of struvite and biorecovery of cerium by Aspergillus niger

Kang

Csetényi

Gao

et al. 2022

Appl Microbiol Biotechnol

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Cerium has many modern applications such as in renewable energies and the biosynthesis of nanomaterials. In this research, natural struvite was solubilized by Aspergillus niger and the biomass-free struvite leachate was investigated for its ability to recover cerium. It was shown that struvite was completed solubilized following 2 weeks of fungal growth, which released inorganic phosphate (Pi) from the mineral by the production of oxalic acid. Scanning electron microscopy (SEM) showed that crystals with distinctive morphologies were formed in the natural struvite leachate after mixing with Ce3+. Energy-dispersive X-ray analysis (EDXA), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) confirmed the formation of cerium phosphate hydrate [Ce(PO4)·H2O] at lower Ce concentrations and a mixture of phosphate and cerium oxalate decahydrate [Ce2(C2O4)3·10H2O] at higher Ce concentrations. The formation of these biogenic Ce minerals leads to the removal of > 99% Ce from solution. Thermal decomposition experiments showed that the biogenic Ce phosphates could be transformed into a mixture of CePO4 and CeO2 (cerianite) after heat treatment at 1000 °C. These results provide a new perspective of the fungal biotransformation of soluble REE species using struvite leachate, and also indicate the potential of using the recovered REE as biomaterial precursors with possible applications in the biosynthesis of novel nanomaterials, elemental recycling and biorecovery. Key points • Cerium was recovered using a struvite leachate produced by A. niger. • Oxalic acid played a major role in struvite solubilization and Ce phosphate biorecovery. • Resulting nanoscale mineral products could serve as a precursor for Ce oxide synthesis.

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“…Therefore, researchers began to pay attention to the biomineralization of microorganisms. 7,8 Magnetotactic bacteria could synthesize magnetite crystals with specific sizes and shapes (such as cubic octahedron, elongated hexahedron, and bullet type) in their cells under mild conditions. 9 Some proteins play an important role in the magnetite crystal biomineralization process by wrapping them in magnetosomal membranes containing these proteins.…”

Section: Introductionmentioning

confidence: 99%

One-Pot Synthesis of Multiple Stimuli-Responsive Magnetic Nanomaterials Based on the Biomineralization of Elastin-like Polypeptides

et al. 2021

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Synthesis of multiple stimuli-responsive magnetic nanomaterials in a green way remains as a big challenge currently. Herein, temperature-responsive elastin-like polypeptides (ELPs) were designed to involve in the biomimetic mineralization and successfully prepared magnetic nanoparticles (MNPs) (named ELPs-MNPs) with multiple responsiveness (temperature, magnetic, and biomimetic silicification responsiveness) in one pot. ELPs-MNPs were identified as cubic nanomaterials with an average size of about 32 nm and in line with the classic ferromagnetic behavior. Interestingly, ELPs-MNPs show clearly lower critical solution temperature phase behavior with a transition temperature of 36 °C. Moreover, ELPs-MNPs can spontaneously trigger the biosilicification of tetramethyl orthosilicate (TMOS) to entrap themselves into silicon oxide as proved by the Fourier transform infrared spectra (FTIR) and elemental mapping of transmission electron microscopy (TEM), with an average size of about 62 nm. The possible role of ELPs in the biomimetic preparation of the multiple stimuli-responsive MNPs was also addressed. The proposed novel and simple one-pot strategy to synthesize multifunctional nanomaterials with higher effectiveness is the first report for preparing MNPs with multiple stimuli response. This strategy conforms to the concept of green chemistry and will pave a new way for the design of smart biomaterials, which may have great potentials for different fields.

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Role of Protein in Fungal Biomineralization of Copper Carbonate Nanoparticles

Cited by 27 publications

References 49 publications

Application of fungal copper carbonate nanoparticles as environmental catalysts: organic dye degradation and chromate removal

Application of fungal copper carbonate nanoparticles as environmental catalysts: organic dye degradation and chromate removal

Solubilization of struvite and biorecovery of cerium by Aspergillus niger

One-Pot Synthesis of Multiple Stimuli-Responsive Magnetic Nanomaterials Based on the Biomineralization of Elastin-like Polypeptides

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