Role of Proline in Mitigating NaCl induced Oxidative Stress in <i>Eruca sativa</i> Miller: An important Oil Yielding Crop of Indian Thar Desert

Mahawar

Rajput

et al. 2021

Plants

Self Cite

Despite the documented significance of carbon-based nanomaterials (CNMs) in plant development, the knowledge of the impact of carbon nanoparticles (CNPs) dosage on physiological responses of crop plants is still scarce. Hence, the present study investigates the concentration-dependent impact of CNPs on the morphology and physiology of Vigna radiata. Crop seedlings were subjected to CNPs at varying concentrations (25 to 200 µM) in hydroponic medium for 96 h to evaluate various physiological parameters. CNPs at an intermediate concentration (100 to 150 µM) favor the growth of crops by increasing the total chlorophyll content (1.9-fold), protein content (1.14-fold) and plant biomass (fresh weight: 1.2-fold, dry weight: 1.14-fold). The highest activity of antioxidants (SOD, GOPX, APX and proline) was also recorded at these concentrations, which indicates a decline in ROS level at 100 µM. At the highest CNPs treatment (200 µM), aggregation of CNPs was observed more on the root surface and accumulated in higher concentrations in the plant tissues, which limits the absorption and translocation of nutrients to plants, and hence, at these concentrations, the oxidative damage imposed by CNPs is evaded with the rise in activity of antioxidants. These findings show the importance of CNPs as nano-fertilizers that not only improve plant growth by their slow and controlled release of nutrients, but also enhance the stress-tolerant and phytoremediation efficiency of plants in the polluted environment due to their enormous absorption potential.

Section: Discussionsupporting

confidence: 92%

Role of Engineered Carbon Nanoparticles (CNPs) in Promoting Growth and Metabolism of Vigna radiata (L.) Wilczek: Insights into the Biochemical and Physiological Responses

Mahawar

Rajput

et al. 2021

Plants

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“…3). The enhancement of resistance towards oxidative stress was likely to correlate with ROS detoxifying ability of proline (Tripathi et al 2013;Mahawar et al 2018b).…”

Section: Discussionmentioning

confidence: 99%

EsHO 1 mediated mitigation of NaCl induced oxidative stress and correlation between ROS, antioxidants and HO 1 in seedlings of Eruca sativa: underutilized oil yielding crop of arid region

Mahawar

Physiol Mol Biol Plants

2019

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Study have focused on NaCl induced HO 1 production and its co-relation to ROS and antioxidant regulation in Eruca sativa. Seedlings were subjected to NaCl stress ranges from 10 to 150 mM. After 96 h of treatment, plants samples were harvested to evaluate the cellular equilibrium and salt tolerance mechanisms through morphological, stress parameters, non enzymatic and antioxidant enzymes. The HO 1 activity was found to be highest at 75 mM NaCl in leaves and roots which were 2.49 and 2.02 folds respectively. The expression of EsHO 1 was also observed and the higher expression was recorded in roots than leaves. The overall activity of other antioxidants (APX and proline) was also found to be higher at 75 mM concentration. The highest HO 1 activity with other antioxidants indicates the decline in LPX and ROS at 75 mM NaCl. The present study concluded that HO 1 helps in amelioration of NaCl stress by working within a group of antioxidants that create the defense machinery in seedlings of E. sativa by manipulating various physiological processes of plants. These findings for the first time suggest the protective role of HO 1 in scavenging ROS in E. sativa under salinity stress.

“…To minimize the oxidative stress, plants have developed potential antioxidant defense system to counteract ROS mediated toxicity (Verma et al 2008;Shekhawat et al 2008Shekhawat et al , 2009Shekhawat et al , 2010Shekhawat et al , 2019Khator and Shekhawat 2020). The antioxidant defense system comprises non-enzymatic antioxidants which include; ascorbic acid (AsA), glutathione (GSH), proline, phenolic compounds, alkaloids, non-protein amino acids, and α-tocopherols and enzymatic antioxidants include; superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), guaiacol peroxidase (GPX) and glutathione S-transferase (GST) (Hasanuzzaman et al 2012;Verma and Shekhawat 2012;Khator and Shekhawat 2018;Mahawar and Shekhawat 2018;Mahawar et al 2018a). Hence, cellular detoxification is the most important tolerance mechanism to overcome salt-induced oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide mitigates salt-induced oxidative stress in Brassica juncea seedlings by regulating ROS metabolism and antioxidant defense system

Khator

2020

3 Biotech

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The present investigation was designed to determine the interaction of nitric oxide with other antioxidants in relieving oxidative stress induced by NaCl at morphological, physiological and molecular level. 15 days old seedlings of B. juncea were subjected to 50 mM NaCl alone, 100 μM SNP alone and in combination (SNP + NaCl) in hoagland growth medium for 96 h and to analyze the cellular homeostasis and salt tolerance mechanism via examining growth, stress parameters, enzymatic and non enzymatic antioxidants and expression level of NR. Exposure of 100 μM sodium nitroprusside to mustard seedling enhanced photosynthetic pigment content and prevented plant growth inhibition. Accumulation of MDA and H 2 O 2 was more pronounced in individual NaCl treated seedling than in the combination of NaCl and SNP. Applying SNP enhanced NR activity by 1.70 folds and increased NO production by 2.26 folds than individual salt treated roots. Furthermore, the activities of CAT, GPX and NR act synergistically with endogenous NO level whereas APX work antagonistically. In addition, the study also demonstrates that NO regulated NaCl induced transcriptional expression of NR. Induction of BjNR in Indian mustard roots lead to enhanced the plant resistance against salinity stress. The present finding revealed that NO confers increased B. juncea tolerance to salt stress by stimulation of antioxidants and reestablishment of cellular redox status.