Role of prokaryotic type I and III pantothenate kinases in the coenzyme A biosynthetic pathway of<i>Bacillus subtilis</i>

Ogata, Yuta; Katoh, Hiroki; Asayama, Munehiko; Chohnan, Shigeru

doi:10.1139/cjm-2013-0793

Cited by 10 publications

(8 citation statements)

References 33 publications

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“…The addition of gluconates into the medium may also increase the basal level of acetyl-CoA following glycolysis, resulting in the elevated production of PHB. An increase in the production of CoA may also be effective for the overproduction of PHB in the biosynthetic pathway (29). Another approach using synergistic effects, involving increases in the expression of the RNA polymerase sigma factor SigE in the transconjugant 6803GT_ABEC, which may be advantageous for the further overproduction of PHB in future studies (11,30).…”

Section: Metabolite Analysis In Phb Overproductionmentioning

confidence: 99%

Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria

Hondo

Takahashi

Osanai

et al. 2015

Journal of Bioscience and Bioengineering

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Genetic engineering and metabolite profiling for the overproduction of polyhydroxybutyrate (PHB), which is a carbon material in biodegradable plastics, were examined in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Transconjugants harboring cyanobacterial expression vectors that carried the pha genes for PHB biosynthesis were constructed. The overproduction of PHB by the engineering cells was confirmed through microscopic observations using Nile red, transmission electron microscopy (TEM), or nuclear magnetic resonance (NMR). We successfully recovered PHB from transconjugants prepared from nitrogen-depleted medium without sugar supplementation in which PHB reached approximately 7% (w/w) of the dry cell weight, showing a value of 12-fold higher productivity in the transconjugant than that in the control strain. We also measured the intracellular levels of acetyl-CoA, acetoacetyl-CoA, and 3-hydroxybutyryl-CoA (3HB-CoA), which are intermediate products for PHB. The results obtained indicated that these products were absent or at markedly low levels when cells were subjected to the steady-state growth phase of cultivation under nitrogen depletion for the overproduction of bioplastics. Based on these results, efficient factors were discussed for the overproduction of PHB in recombinant cyanobacteria.

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Section: Metabolite Analysis In Phb Overproductionmentioning

confidence: 99%

Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria

Hondo

Takahashi

Osanai

et al. 2015

Journal of Bioscience and Bioengineering

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“…C. ammoniagenes carries a type I coaA which is highly regulated by CoA and its derivatives. In contrast, type II and III enzymes are insensitive to CoA and its thioesters [27, 28], therefore, in order to reduce feedback inhibition and increase the production of CoA, the type III pantothenate kinase from Pseudomonas putida ( Pp coaA) was selected and overexpressed by the control of the strongest promoter P rpl21 in C. ammoniagenes ATCC 6871 [29, 30].…”

Section: Resultsmentioning

confidence: 99%

Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis

et al. 2019

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BackgroundCorynebacterium ammoniagenes is an important industrial organism that is widely used to produce nucleotides and the potential for industrial production of coenzyme A by C. ammoniagenes ATCC 6871 has been shown. However, the yield of coenzyme A needs to be improved, and the available constitutive promoters are rather limited in this strain.ResultsIn this study, 20 putative DNA promoters derived from genes with high transcription levels and 6 promoters from molecular chaperone genes were identified. To evaluate the activity of each promoter, red fluorescence protein (RFP) was used as a reporter. We successfully isolated a range of promoters with different activity levels, and among these a fragment derived from the upstream sequence of the 50S ribosomal protein L21 (Prpl21) exhibited the strongest activity among the 26 identified promoters. Furthermore, type III pantothenate kinase from Pseudomonas putida (PpcoaA) was overexpressed in C. ammoniagenes under the control of Prpl21, CoA yield increased approximately 4.4 times.ConclusionsThis study provides a paradigm for rational isolation of promoters with different activities and their application in metabolic engineering. These promoters will enrich the available promoter toolkit for C. ammoniagenes and should be valuable in current platforms for metabolic engineering and synthetic biology for the optimization of pathways to extend the product spectrum or improve the productivity in C. ammoniagenes ATCC 6871 for industrial applications.

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“…Previously, it has also been shown that E. coli mutants constitutively expressing DsdA are able to use D-serine as the sole source of carbon and nitrogen (Bloom and McFall, 1975). In B. subtlilis the dsdA gene is located in the yqjP - yqjQ - dsdA - coaA - yqjT operon, containing three genes of unknown function as well as the dsdA and coaA genes, of which the latter encodes the major pantothenate kinase CoaA (Ogata et al, 2014). The genetic context of the dsdA gene strongly suggests that the D-serine deaminase may be involved in the detoxification of D-serine that probably also interferes with pantothenate synthesis in B. subtilis .…”

Section: Poorly Characterized Plp-dependent Enzymesmentioning

confidence: 99%

A Survey of Pyridoxal 5′-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis

Richts

Rosenberg

Commichau

2019

Front. Mol. Biosci.

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The B6 vitamer pyridoxal 5′-phosphate (PLP) is a co-factor for proteins and enzymes that are involved in diverse cellular processes. Therefore, PLP is essential for organisms from all kingdoms of life. Here we provide an overview about the PLP-dependent proteins from the Gram-positive soil bacterium Bacillus subtilis . Since B. subtilis serves as a model system in basic research and as a production host in industry, knowledge about the PLP-dependent proteins could facilitate engineering the bacteria for biotechnological applications. The survey revealed that the majority of the PLP-dependent proteins are involved in metabolic pathways like amino acid biosynthesis and degradation, biosynthesis of antibacterial compounds, utilization of nucleotides as well as in iron and carbon metabolism. Many PLP-dependent proteins participate in de novo synthesis of the co-factors biotin, folate, heme, and NAD + as well as in cell wall metabolism, tRNA modification, regulation of gene expression, sporulation, and biofilm formation. A surprisingly large group of PLP-dependent proteins (29%) belong to the group of poorly characterized proteins. This review underpins the need to characterize the PLP-dependent proteins of unknown function to fully understand the “PLP-ome” of B. subtilis .

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Role of prokaryotic type I and III pantothenate kinases in the coenzyme A biosynthetic pathway ofBacillus subtilis

Cited by 10 publications

References 33 publications

Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria

Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria

Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis

A Survey of Pyridoxal 5′-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis

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