Role of porcine endometrial estrogen sulfotransferase in progesterone mediated downregulation of estrogen receptor

Saunders, Dwight E.; Lozon, Marie M.; Corombos, J.D.; Brooks, S.C.

doi:10.1016/0022-4731(89)90450-0

Cited by 14 publications

(9 citation statements)

References 29 publications

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“…In general, oestrogen is suggested to up‐regulate and progesterone down‐regulate the ER α gene expression in the endometrium of female mammals as shown for different species (Saunders et al. 1989; Bergman et al.…”

Section: The Oestrogen Receptor α (Erα)mentioning

confidence: 99%

“…1999; Ing and Ott 1999). Saunders et al. (1989) suggested that progesterone may inactivate oestradiol (E 2 ) via interference with E 2 dependent ER, therefore a downregulation of ER follows.…”

Section: The Oestrogen Receptor α (Erα)mentioning

confidence: 99%

“…In a study using oestrogen receptor knockout mice, it was shown that ERa is the major subtype regarding regulation of uterine activities (Couse and Korach 1999;Muramatsu and Inoue, 2000). In pigs, most of the studies on ER in the reproductive organs have been done in gilts (Pack et al 1978;Stanchev et al 1985Stanchev et al , 1990Saunders et al 1989;Geisert et al 1993;Tarleton et al 1998). In sows, having experienced pregnancies, it has earlier been shown that the uterine ER concentration (binding assay) was about three times higher compared with cycling young gilts (Koziorowski et al 1984).…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical Studies on Oestrogen Receptor Alpha (ERα) and the Proliferative Marker Ki‐67 in the Sow Uterus at Different Stages of the Oestrous Cycle

Sukjumlong

Kaeoket

Dalin

et al. 2003

Reprod Domestic Animals

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In order to better understand physiological changes during the different stages of the oestrous cycle, immunohistochemistry was used in the present study to investigate the distribution of oestrogen receptor alpha (ER alpha) as well as the proliferative marker Ki-67, in the sow uterus during the oestrous cycle. Uterine samples were collected from multiparous sows with normal reproductive performance at selected stages of the oestrous cycle: at late dioestrus (d 17), prooestrus (d 19), oestrous (d 1), early dioestrus (d 4) and dioestrus (d 11-12), respectively. The tissue samples were fixed in 10% formaldehyde, embedded in paraffin and subjected to immunohistochemistry using monoclonal antibodies against ER alpha (C-311) and Ki-67 (MM-1). In general, the immunostaining of both ER alpha and Ki-67 was confined to nuclei of the target cells. Variations were seen, not only at the different stages of the oestrous cycle, but also in the different tissue compartments of the uterus. In the epithelia, the strongest ER alpha staining and highest amount of positive Ki-67 cells were found at early dioestrus. In the myometrium, the highest levels of staining of both ER alpha and Ki-67 positive cells were found at pro-oestrus and oestrus. For the proliferative marker, Ki-67, no positive cells were found at dioestrus and late dioestrus in the epithelium and myometrium. In the connective tissue stroma (subepithelial layer), the highest number of ER alpha positive cells were found at oestrus, which was significantly different compared with other stages (p< or = 0.05), whereas the levels of Ki-67 positive cells were relatively low and did not differ between the stages examined. Significant correlations between the number of ER alpha positive cells in the stroma and Ki-67 positive cells in the epithelia were observed. This suggests indirect regulatory mechanisms on epithelial proliferation via ER alpha in the stroma. In conclusion, these findings in the sow uterus show that the presence of ER alpha as well as Ki-67 protein varies not only between different stages of the oestrous cycle but also between different tissue compartments of the uterus. These findings indicate various regulatory mechanisms and stress the importance of localising ER alpha and proliferating cells in different uterine tissues.

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Section: The Oestrogen Receptor α (Erα)mentioning

confidence: 99%

Section: The Oestrogen Receptor α (Erα)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical Studies on Oestrogen Receptor Alpha (ERα) and the Proliferative Marker Ki‐67 in the Sow Uterus at Different Stages of the Oestrous Cycle

Sukjumlong

Kaeoket

Dalin

et al. 2003

Reprod Domestic Animals

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“…Steroid sulfonation, however, can indirectly influence the action of steroid hormones on transcriptional regulation by controlling the level of the unconjugated hormone that can bind to its receptor molecule. For example, the sulfonation of estrogens in uterine (180,211) and breast (212,213) tissue has a significant influence on the level of unconjugated 17/3-estradiol available for binding to its cognate receptor. With regard to breast tissue, EST activity in cultured normal breast epithelial cells is high and exceeds that found in tumor cell lines (128).…”

Section: B Steroid Sulfonates In Steroid Actionmentioning

confidence: 99%

Steroid Sulfotransferases

Strott¹

1996

Endocrine Reviews

162

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“…The enzyme appears to be important in reproductive/ developmental tissues, and has been studied in placenta , chorion (Bartlett and Hobkirk, 1991), endometrium (Saunders et al, 1989) and testes (Hobkirk et al, 1989). Additionally, Hobkirk's group has determined that, in the guinea pig adrenal gland, there are separate STs for the sulphation of oestrogens and hydroxysteroids such as pregnenolone (Hobkirk et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Purification and immunochemical characterization of a male-specific rat liver oestrogen sulphotransferase

Ltd¹

1993

Biochemical Journal

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Role of porcine endometrial estrogen sulfotransferase in progesterone mediated downregulation of estrogen receptor

Cited by 14 publications

References 29 publications

Immunohistochemical Studies on Oestrogen Receptor Alpha (ERα) and the Proliferative Marker Ki‐67 in the Sow Uterus at Different Stages of the Oestrous Cycle

Immunohistochemical Studies on Oestrogen Receptor Alpha (ERα) and the Proliferative Marker Ki‐67 in the Sow Uterus at Different Stages of the Oestrous Cycle

Steroid Sulfotransferases

Purification and immunochemical characterization of a male-specific rat liver oestrogen sulphotransferase

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