Role of polymorphic variants of gene of inducible NO synthase NOS2 in brain infarction in patients with acute ischemic stroke

Tupitsyna, T. V.; Bondarenko, Elena; Botsina, A. Yu.; Шетова, И. М.; Limborskaya, S. A.; Skvortsova, Veronika; Сломинский, П. А.

doi:10.3103/s0891416810030018

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…Oxygen-free radicals and oxidants appear to play an essential role in central nervous system injury after cerebral ischemia and reperfusion. The excessive production of NO with the further formation of the toxic compound peroxynitrite anion ONOO – , is currently considered to be one of the most important mechanisms of tissue damage at various stages of brain ischemia [35,36,37]. The quantity of NO synthesized by NOS2 is thousands of times higher than the total quantity of NO formed by NO synthase.…”

Section: Discussionmentioning

confidence: 99%

“…The quantity of NO synthesized by NOS2 is thousands of times higher than the total quantity of NO formed by NO synthase. The expression of NOS2 in the cells of ischemic tissue and the surrounding potentially viable zone has been considered to be one of the factors that determine the distribution of tissue damage at the later stages of cerebral ischemia [37].…”

Section: Discussionmentioning

confidence: 99%

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Investigating the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat Using Metabolomics and Network Pharmacology

et al. 2019

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Stroke is one of the leading causes of death and long-term disability worldwide. Gross saponins of Tribulus terrestris fruit (GSTTF) has been used for neuroprotective therapy on convalescents of ischemic stroke. But the related therapeutic mechanisms have not yet been well investigated. This study aimed to investigate the protective effects of GSTTF on ischemic stroke using metabolomics coupled with network pharmacology analysis. The rat urine sample was collected and profiled by an LC-MS-based metabolomics approach. The pathway analysis was performed based on the highlighted biomarkers, then the network pharmacology approach was applied to screen the potential therapeutic targets of GSTTF. Metabolomics analysis showed that a series of metabolic perturbations occurred in the middle cerebral artery occlusion (MCAO) group compared with the sham group. Gross saponins of Tribulus terrestris fruit can change the MCAO-induced urine metabolic deviations in a reverse manner via regulating multiple metabolic pathways. Two proteins, inducible nitric oxide synthase (NOS2) and glycogen synthase kinase-3 beta (GSK3B), were highlighted by the network pharmacology analysis, which may be the potential therapeutic targets for the GSTTF against ischemic stroke. This study provides an overview of the mechanism of MCAO-induced ischemic stroke and investigates the efficacy of GSTTF in the treatment of ischemic stroke. Further study is needed to reveal its underlying mechanisms more clearly.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Investigating the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat Using Metabolomics and Network Pharmacology

et al. 2019

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Multilocus analysis of the association of polymorphic variants of inflammation genes with ischemic stroke in Russians

et al. 2016

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Evidence of Redox Unbalance in Post-Acute Ischemic Stroke Patients

Ciancarelli

Massimo²,

Amicis³

et al. 2012

CNR

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Measurements of the redox balance after the ischemic stroke occurrence might be useful to monitor the outcome of patients who suffered an ischemic stroke in terms of stroke recurrence and other vascular events. For this purpose, fifteen patients (mean age 71.40±2.50 years) with a first-ever ischemic stroke were included in the study within 30 days of stroke onset. Stroke severity was evaluated according to the National Institutes of Health Stroke Scale (NIHSS). Redox balance was assessed by measuring plasma amount of total peroxidative by-products, nitrite/nitrate metabolites (NOx), as expression of nitric oxide (NO) plasma bioavailability, total plasma antioxidant capacity (TEAC), Cu/Zn Superoxide Dismutase (Cu/Zn SOD) activity, serum urate concentration and autoantibodies against ox-LDL (OLAB) serum level. Total cholesterol, triglycerides, high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were also measured. Fifteen apparently healthy controls (mean age 70.28±2.03 years) were investigated to compare redox markers. Stroke patients had higher plasma values of total peroxidative by-products, NOx stable metabolites and of total cholesterol, triglycerides, and LDL-C than controls (P < 0.05). No differences in OLAB levels, Cu/Zn-SOD activity, serum urate concentration, and plasma HDL-C amount were found in stroke patients when compared to controls. Total plasma antioxidant capacity was lower in stroke patients than in controls. NOx values correlated positively with the NIHSS score in stroke patients (r=0.668; P=0.0065). The observed presence of redox unbalance in stroke patients could represent an early indicator of diffuse endothelial activation during which patients may be at increased risk of stroke recurrence and other vascular events.

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Role of polymorphic variants of gene of inducible NO synthase NOS2 in brain infarction in patients with acute ischemic stroke

Cited by 4 publications

References 16 publications

Investigating the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat Using Metabolomics and Network Pharmacology

Investigating the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat Using Metabolomics and Network Pharmacology

Multilocus analysis of the association of polymorphic variants of inflammation genes with ischemic stroke in Russians

Evidence of Redox Unbalance in Post-Acute Ischemic Stroke Patients

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