Role of poly(ADP-ribose) polymerases in male reproduction

Çelik-Özenci, Çiler; Taşatargil, Arda

doi:10.4161/spmg.24194

Cited by 29 publications

(26 citation statements)

References 90 publications

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“…The current result is in agreement with these obtained by Ahmed and Tawfik (2012), who verified the effect of γ-irradiation and the abnormal forms of sperm (sperm head abnormalities) due to the spermatogenesis disruption. Also, seminal-OS reduced sperm motility and function through damaging its DNA and membrane integrity (Celik-Ozenci and Tasatargil, 2013). Alternatively, treatment of rats with FA post γ-irradiation ameliorated this significant elevation in sperm head abnormalities toward normal value.…”

Section: Discussionmentioning

confidence: 93%

The Potential Therapeutic Effect of Ferulic Acid in Mitigating Oxidative Stress Induced by γ-Irradiation in Male Rats

2016

Egypt. J. Rad.Sci.

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HE TESTIS is considered as one of the most sensitive organs in the body to ionizing radiation because of the rapidly dividing germinal epithelium. Seminal oxidative stress (OS) causes damage of the sperm plasma membrane and loss of its DNA integrity, therefore, the need for an effective therapeutic agent is evident. The present study investigated the mechanism(s) of potential therapeutic effect of ferulic acid (FA) on radiation-induced testicular damage.Mature male albino rats were either exposed to single dose γ-radiation (5Gy) and/ or treated with FA (50mg/ kg body wt, orally), daily for seven days post-irradiation. FA significantly reversed OS effects of γ-rays that was evidenced by increasing malondialdehyde (MDA) and decreasing ferric reducing antioxidant power (FRAP) and catalase (CAT) activity. In addition, alterations in some trace elements such as zinc (Zn), cupper (Cu), iron (Fe) and manganese (Mn) were observed. Furthermore, sperm head abnormalities noticeably increased in the γ-irradiated group; in contrast, FA treatment ameliorated these alterations.In conclusion, FA exhibited curative effect against radiation induced testicular damage.

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Section: Discussionmentioning

confidence: 93%

The Potential Therapeutic Effect of Ferulic Acid in Mitigating Oxidative Stress Induced by γ-Irradiation in Male Rats

2016

Egypt. J. Rad.Sci.

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“…The PARP2, PARP9 and PARP14 genes are members of poly (ADP‐ribose) polymerase (PARP) family which plays a crucial role in maintaining the genomic integrity of several cell types acting in DNA repair, maintenance of genomic stability, transcriptional regulation, apoptosis and necrosis (Ame, Spenlehauer, & Murcia, ; Celik‐Ozenci & Tasatargil, ; Said & Khosravi, ). Maintaining the integrity of sperm DNA is vital to male fertility and the PARPs have several roles during spermatogenesis, sperm maturation and even in ejaculated sperm through protection against chemically induced damage (Agarwal et al, ; Celik‐Ozenci & Tasatargil, ).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide association study for age at puberty in young Nelore bulls

Stafuzza

Silva

Silva³

et al. 2019

J Animal Breeding Genetics

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Selection for bulls that would reach puberty early reduces the generation interval and increases fertility and herd productivity. Despite its economic importance, there are few QTL associated with age at puberty described in the literature. In this study, a weighted single‐step genome‐wide association study was performed to detect genomic regions and putative candidate genes related to age at puberty in young Nelore bulls. Several protein‐coding genes related to spermatogenesis functions were identified within the genomic regions that explain more than 0.5% of the additive genetic variance for age at puberty in Nelore bulls, such as ADAM11, BRCA1, CSNK2A, CREBBP, MEIOC, NDRG2, NECTIN3, PARP2, PARP9, PRSS21, RAD51C, RNASE4, SLX4, SPA17, TEX14, TIMP2 and TRIP13 gene. Enrichment analysis by DAVID also revealed several GO terms related to spermatogenesis such as DNA replication (GO:0006260), male meiosis I (GO:0007141), double‐strand break repair (GO:0006302), base excision repair (GO:0006284), apoptotic process (GO:0006915), cell–cell adhesion (GO: 0098609) and focal adhesion (GO:0005925). The heritability for age at puberty shows that this trait can be improved based on traditional EBV selection. Adding genomic information to the system helps to elucidate genes and molecular mechanisms controlling the sexual precocity and could help to predict sexual precocity in Nelore bulls with greater accuracy at younger age, which would speed up the breeding programme for this breed.

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“…Some members of this family contribute to both DNA repair and cell death (Agarwal et al. ), and they also play important roles at different stages of spermatogenesis (Celik‐Ozenci and Tasatargil ). Jha et al.…”

Section: Introductionmentioning

confidence: 99%

Cleaved PARP‐1, an Apoptotic Marker, can be Detected in Ram Spermatozoa

Casao

Mata-Campuzano

Ordás

et al. 2015

Reprod Domestic Animals

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The presence of apoptotic features in spermatozoa has been related to lower quality and functional impairment. Members of the poly-ADP-ribose polymerases (PARP) familyare involved in both DNA repair and apoptosis, playing important roles in spermatogenesis. Poly-ADP-ribose polymerase can be cleaved by caspases, and the presence of its cleavage product (cPARP) in spermatozoa has been related to chromatin remodelling during spermatogenesis and to the activation of apoptotic pathways. There are no reports on immunodetection of cPARP in ram spermatozoa; thus, we have tested a commercially available antibody for this purpose. cPARP was microscopically detected in the acrosomal ridge of some spermatozoa (indirect immunofluorescence). A preliminary study was carried out by flow cytometry (direct immunofluorescence, FITC). Ram semen was extended in TALP and incubated for 4 h with apoptosis inducers staurosporine (10 μm) or betulinic acid (200 μm). Both inducers and incubation caused a significant increase in cPARP spermatozoa (0 h, control: 21.4±3.3%, inducers: 44.3±1.4%; 4 h, control: 44.3±2.4%, inducers: 53.3±1.4%). In a second experiment, we compared the sperm fractions after density gradient separation (pellet and interface). The pellet yielded a slightly lower proportion of cPARP spermatozoa (28.5±1.2% vs 36.2±2.0% in the interface; p < 0.001), and a 12-h incubation increased cPARP similarly in both fractions (p < 0.001). cPARP seems to be an early marker of apoptosis in ram semen, although its presence in untreated samples was weakly related to worse quality (pellet/interface). We suggest to study the relationship of PARP and cPARP levels with between-male differences on sperm fertility.

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Role of poly(ADP-ribose) polymerases in male reproduction

Cited by 29 publications

References 90 publications

The Potential Therapeutic Effect of Ferulic Acid in Mitigating Oxidative Stress Induced by γ-Irradiation in Male Rats

The Potential Therapeutic Effect of Ferulic Acid in Mitigating Oxidative Stress Induced by γ-Irradiation in Male Rats

Genome‐wide association study for age at puberty in young Nelore bulls

Cleaved PARP‐1, an Apoptotic Marker, can be Detected in Ram Spermatozoa

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