Role of Plasmids in Co-Selection of Antimicrobial Resistances Among <i>Escherichia coli</i> Isolated from Pigs

Ozawa, Manao; Shirakawa, Takahiro; Moriya, Kazuo; Furuya, Yukari; Kawanishi, Michihiro; Makita, Kohei; Sekiguchi, Hideto

doi:10.1089/fpd.2023.0021

Cited by 2 publications

(1 citation statement)

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“…In certain plasmids (e.g., IncX plasmid), chloramphenicol resistance genes were preserved, rather than in others [16]. These genes were found co-located on the same conjugative plasmid with the other AMR genes such as tet, aadA and sul, conferring multidrug resistance phenotype [17]. Despite being banned in food animals, chloramphenicol is used topically to treat eye infections in humans.…”

Section: Introductionmentioning

confidence: 99%

Molecular basis of the persistence of chloramphenicol resistance among Escherichia coli and Salmonella spp. from pigs, pork and humans in Thailand

Puangseree,

Prathan,

Srisanga

et al. 2024

PLoS ONE

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This study aimed to investigate the potential mechanisms associated with the persistence of chloramphenicol (CHP) resistance in Escherichia coli and Salmonella enterica isolated from pigs, pork, and humans in Thailand. The CHP-resistant E. coli (n = 106) and Salmonella (n = 57) isolates were tested for their CHP susceptibility in the presence and absence of phenylalanine arginine β-naphthylamide (PAβN). The potential co-selection of CHP resistance was investigated through conjugation experiments. Whole genome sequencing (WGS) was performed to analyze the E. coli (E329, E333, and E290) and Salmonella (SA448, SA461, and SA515) isolates with high CHP MIC (32–256 μg/mL) and predominant plasmid replicon types. The presence of PAβN significantly reduced the CHP MICs (≥4-fold) in most E. coli (67.9%) and Salmonella (64.9%). Ampicillin, tetracycline, and streptomycin co-selected for CHP-resistant Salmonella and E. coli-transconjugants carrying cmlA. IncF plasmids were mostly detected in cmlA carrying Salmonella (IncFIIAs) and E. coli (IncFIB and IncF) transconjugants. The WGS analysis revealed that class1 integrons with cmlA1 gene cassette flanked by IS26 and TnAs1 were located on IncX1 plasmid, IncFIA(HI1)/HI1B plasmids and IncFII/FIB plasmids. IncFIA(HI1)/HI1B/Q1in SA448 contained catA flanked by IS1B and TnAs3. In conclusion, cross resistance through proton motive force-dependent mechanisms and co-selection by other antimicrobial agents involved the persistence of CHP-resistance in E. coli in this collection. Dissemination of CHP-resistance genes was potentially facilitated by mobilization via mobile genetic elements.

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Section: Introductionmentioning

confidence: 99%