Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response

Wang, Wang‐Xia; Vinocur, Basia; Shoseyov, Oded; Altman, Arie

doi:10.1016/j.tplants.2004.03.006

Cited by 2,281 publications

(1,792 citation statements)

References 94 publications

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“…The HSP100 family operates in protein disaggregation and/ or protein degradation. The removal of nonfunctional, but potentially harmful polypeptides arising from misfolding, denaturation, or aggregation is important for the maintenance of cellular homeostasis [31]. It also has been reported that transgenic plants overexpressing a high molecular HSP (hsp 101) gene had enhanced thermotolerance [6].…”

Section: Hspsmentioning

confidence: 99%

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

et al. 2007

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The present study investigated rice leaf proteome in response to heat stress. Rice seedlings were subjected to a temperature of 427C and samples were collected 12 and 24 h after treatment. Increased relative ion leakage and lipid peroxidation suggested that oxidative stress frequently was generated in rice leaves exposed to high temperature. 2-DE, coupled with MS, was used to investigate and identify heat-responsive proteins in rice leaves. In order to identify the low-abundant proteins in leaves, samples were prefractionated by 15% PEG. The PEG supernatant and the pellet fraction samples were separated by 2-DE, and visualized by silver or CBB staining. Approximately 1000 protein spots were reproducibly detected on each gel, wherein 73 protein spots were differentially expressed at least at one time point. Of these differentially expressed proteins, a total of 34 and 39 protein spots were found in the PEG supernatant and pellet fractions, respectively. Using MALDI-TOF MS, a total of 48 proteins were identified. These proteins were categorized into classes related to heat shock proteins, energy and metabolism, redox homeostasis, and regulatory proteins. The results of the present study show that a group of low molecular small heat shock proteins (sHSPs) were newly induced by heat stress. Among these sHSPs, a low molecular weight mitochondrial (Mt) sHSP was validated further by Western blot analysis. Furthermore, four differentially accumulated proteins that correspond to antioxidant enzymes were analyzed at the mRNA level, which confirmed the differential gene expression levels, and revealed that transcription levels were not completely concomitant with translation. The identification of some novel proteins in the heat stress response provides new insights that can lead to a better understanding of the molecular basis of heat-sensitivity in plants.

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Section: Hspsmentioning

confidence: 99%

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

et al. 2007

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“…HSPs play an important role in cellular protection under stress in addition to participation in developmental processes, such as embryogenesis, embryo development, seed germination, pollen development, and fruit maturation (Ingram and Bartels, 1996;Waters et al, 1996;Wang et al, 2004). HSPs as molecular chaperones engage in protein synthesis, targeting, maturation and degradation under normal and stress conditions and have been classified accordingly (Waters et al, 1996;Wang et al, 2004).…”

Section: Hsp's As a Molecular Chaperonementioning

confidence: 99%

“…HSPs as molecular chaperones engage in protein synthesis, targeting, maturation and degradation under normal and stress conditions and have been classified accordingly (Waters et al, 1996;Wang et al, 2004). In plants, the small heat-shock proteins (SHSPs) are most prevalent among the five conserved families of HSPs (HSP100, HSP90, HSP70, HSP60 and SHSP).…”

Section: Hsp's As a Molecular Chaperonementioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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“…Cd-TDF 81, homologous to a heat shock protein 70 (Hsp70) gene could play a role in the protection of proteins upon Cd exposure. Hsp proteins are chaperones involved in protein folding which were often shown to be upregulated in response to stress (Wang et al 2004;Muller et al 2007a;Kieffer et al 2009). Cd-TDF 67 corresponding gene encodes a copper amine oxidase (CAO).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates

et al. 2010

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The ectomycorrhizal basidiomycete Suillus luteus (L.:Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments.

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Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response

Cited by 2,281 publications

References 94 publications

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates

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