Role of physicochemical characteristics in the uptake of TiO2 nanoparticles by fibroblasts

Allouni, Zouhir Ekeland; Høl, Paul Johan; Cauqui, M.A.; Gjerdet, Nils Roar; Cimpan, Mihaela Roxana

doi:10.1016/j.tiv.2012.01.019

Cited by 56 publications

(68 citation statements)

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“…Particles with smaller sizes possess larger surface areas, have a stronger tendency to agglomerate in medium, and interact more with biomolecules such as proteins and DNA in biological environment. These characteristics may contribute to particle uptake and various intracellular responses (Allouni et al, 2012;Horie et al, 2010;Thevenot et al, 2008;Xia et al, 2008). Similarly, our results indicated that smaller TiO 2 particles (nano-TiO 2 ) were taken up by cells more than the largersized particles (micron-sized TiO 2 ), as detected by microscopy ( Fig.…”

Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 79%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

115

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/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://dx.doi.org/10. 1016/j.tiv.2012.12.018 Toxicology in Vitro, 27, 2, pp. 864-873, 2012-12-28 In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells Hamzeh, Mahsa; Sunahara, Geoffrey I. Therefore, our objective was to investigate the cytotoxicity and genotoxicity of commercially available TiO 2 nanoparticles with respect to their selected physicochemical properties, as well as the role of surface coating of these nanoparticles. While all types of tested TiO 2 samples decrease cell viability in a mass-based concentration-and size-dependent manner, the polyacrylate-coated nano-TiO 2 product was only cytotoxic at higher concentrations. A similar pattern of response was observed for induction of apoptosis/necrosis, and no DNA damage was detected in the polyacrylate-coated nano-TiO 2 model. Given the increasing production of TiO 2 nanoparticles, toxicological studies should take into account the physiochemical properties of these nanoparticles that may help researchers to develop new nanoparticles with minimum toxicity.

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Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 79%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

115

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“…Several other studies clearly indicate that size is an important parameter for uptake. 25,[50][51][52] It is difficult to say if the difference in the degree of uptake was size-related in our study, since the primary particle size range was small. 25,[50][51][52] However, small primary NPs are taken up by other mechanisms than larger particles or agglomerates of the same compound.…”

Section: Cellular Uptake Of Tio 2 Npsmentioning

confidence: 70%

“…Their physicochemical characteristics and transmission electron micrographs were reported by us in a previous paper. 25 The NP samples were 100% anatase, except for A.5, which comprised 14% rutile phase. The smallest particles (A.5) had the highest specific surface area (Braunauer-Emmett-Teller, 131 m 2 /g), then A.10 (105 m 2 /g), whereas the largest particles (A.40) had the lowest (49 m 2 /g).…”

Section: Tio 2 Nanopowdersmentioning

confidence: 96%

“…Previously, we have evaluated the effect of physicochemical characteristics of a panel of TiO 2 NPs on uptake by fibroblasts. 25 The aim of the present study was to quantify the individual adsorption of HSA, Glbs, and Fib onto anatase TiO 2 NPs and their effect on uptake by L929 fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

“…Both is the probably the case, as demonstrated by scanning electron microscopy and transmission electron microscopy imaging in our previous study. 25 It seems that both the adsorbed protein and the particles' native surface may mediate the attachment of NPs to cellular membranes. As mentioned earlier in the discussion, serum proteins adsorb onto the surface of both cationic and anionic NPs.…”

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confidence: 99%

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The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

Allouni¹,

Gjerdet²,

Cimpan³

et al. 2015

IJN

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Protein adsorption onto nanoparticles (NPs) in biological fluids has emerged as an important factor when testing biological responses to NPs, as this may influence both uptake and subsequent toxicity. The aim of the present study was to quantify the adsorption of proteins onto TiO 2 NPs and to test the influence on cellular uptake. The surface composition of the particles was characterized by thermal analysis and by X-ray photoelectron spectroscopy. The adsorption of three blood proteins, ie, human serum albumin (HSA), γ-globulins (Glbs), and fibrinogen (Fib), onto three types of anatase NPs of different sizes was quantified for each protein. The concentration of the adsorbed protein was measured by ultraviolet-visible spectrophotometry using the Bradford method. The degree of cellular uptake was quantified by inductivity coupled plasma mass spectroscopy, and visualized by an ultra-high resolution imaging system. The proteins were adsorbed onto all of the anatase NPs. The quantity adsorbed increased with time and was higher for the smaller particles. Fib and Glbs showed the highest affinity to TiO 2 NPs, while the lowest was seen for HSA. The adsorption of proteins affected the surface charge and the hydrodynamic diameter of the NPs in cell culture medium. The degree of particle uptake was highest in protein-free medium and in the presence HSA, followed by culture medium supplemented with Glbs, and lowest in the presence of Fib. The results indicate that the uptake of anatase NPs by fibroblasts is influenced by the identity of the adsorbed protein.

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TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Ibrahim

Schoelermann

Mustafa

et al. 2018

J Biomedical Materials Res

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Human exposure to titanium dioxide nanoparticles (nano-TiO ) is increasing. An internal source of nano-TiO is represented by titanium-based orthopedic and dental implants can release nanoparticles (NPs) upon abrasion. Little is known about how the size of NPs influences their interaction with cytoskeletal protein networks and the functional/homeostatic consequences that might follow at the implant-bone interface with regard to osteoblasts. We investigated the effects of size of anatase nano-TiO on SaOS-2 human osteoblast-like cells exposed to clinically relevant concentrations (0.05, 0.5, 5 mg/L) of 5 and 40 nm spherical nano-TiO . Cell viability and proliferation, adhesion, spread and migration were assessed, as well as the orientation of actin and microtubule cytoskeletal networks. The phosphorylation of focal adhesion kinase (p-FAK ) and the expression of vinculin in response to nano-TiO were also assessed. Treatment with nano-TiO disrupted the actin and microtubule cytoskeletal networks leading to morphological modifications of SaOS-2 cells. The phosphorylation of p-FAK and the expression of vinculin were also modified depending on the particle size, which affected cell adhesion. Consequently, the cell migration was significantly impaired in the 5 nm-exposed cells compared to unexposed cells. The present work shows that the orientation of cytoskeletal networks and the focal adhesion proteins and subsequently the adhesion, spread and migration of SaOS-2 cells were affected by the selected nano-TiO in a size dependent manner. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2582-2593, 2018.

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Role of physicochemical characteristics in the uptake of TiO2 nanoparticles by fibroblasts

Cited by 56 publications

References 38 publications

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

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Role of physicochemical characteristics in the uptake of TiO2 nanoparticles by fibroblasts

Cited by 56 publications

References 38 publications

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

TiO2 nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

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TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner