Role of physical exercise on hepatic insulin, glucocorticoid and inflammatory signaling pathways in an animal model of non-alcoholic steatohepatitis

Passos, Emanuel; Pereira, Cidália; Gonçalves, Inês O.; Rocha-Rodrigues, Sílvia; Silva, N.; Guimarães, João Tiago; Neves, Delminda; Ascensão, António; Magalhães, José; Martins, Maria João

doi:10.1016/j.lfs.2014.12.013

Cited by 22 publications

(27 citation statements)

References 64 publications

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“…In contrast, a recent animal study has shown that acute long-term exercise by swimming for 180 min reduces JNK phosphorylation and improves insulin signaling and sensitivity in adipose tissue from obese rat [ 69 ]. In particular, chronic endurance exercise in form of swimming and treadmill running contributes to a reduction in JNK phosphorylation and improves insulin signaling and sensitivity in adipose and hepatic tissue from obese rats [ 67 – 69 , 85 ].…”

Section: Inflammation-associated Signaling Pathways and Key Playermentioning

confidence: 99%

Influence of Acute and Chronic Exercise on Glucose Uptake

Röhling

Herder

Stemper

et al. 2016

Journal of Diabetes Research

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Insulin resistance plays a key role in the development of type 2 diabetes. It arises from a combination of genetic predisposition and environmental and lifestyle factors including lack of physical exercise and poor nutrition habits. The increased risk of type 2 diabetes is molecularly based on defects in insulin signaling, insulin secretion, and inflammation. The present review aims to give an overview on the molecular mechanisms underlying the uptake of glucose and related signaling pathways after acute and chronic exercise. Physical exercise, as crucial part in the prevention and treatment of diabetes, has marked acute and chronic effects on glucose disposal and related inflammatory signaling pathways. Exercise can stimulate molecular signaling pathways leading to glucose transport into the cell. Furthermore, physical exercise has the potential to modulate inflammatory processes by affecting specific inflammatory signaling pathways which can interfere with signaling pathways of the glucose uptake. The intensity of physical training appears to be the primary determinant of the degree of metabolic improvement modulating the molecular signaling pathways in a dose-response pattern, whereas training modality seems to have a secondary role.

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Section: Inflammation-associated Signaling Pathways and Key Playermentioning

confidence: 99%

Influence of Acute and Chronic Exercise on Glucose Uptake

Röhling

Herder

Stemper

et al. 2016

Journal of Diabetes Research

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“…On the other hand, regular exercise improves immunity 29 through moderate induction of pro-inflammatory cytokines (e.g., TNF-α, IL-1β and IL-6) in skeletal muscle 45 . Reactive oxidative species (ROS) are also produced 46 during exercise, which in turn activate c-Jun N-terminal kinases (JNK) 47 . Collectively, these processes are associated with skeletal muscle injury and tissue repair 30 , which induces myofibrillar protein synthesis and muscle hypertrophy through activation of mammalian target of rapamycin complex 1 (mTORC1) 48 , and promotes organ function 49 50 .…”

Section: Discussionmentioning

confidence: 99%

Effect of Regular Exercise on Inflammation Induced by Drug-resistant Staphylococcus aureus 3089 in ICR mice

Lee

Luchian

Park

2015

Sci Rep

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Obesity is often associated with irregular dietary habits and reduced physical activity. Regular exercise induces a metabolic response that includes increased expression of various cytokines, signaling proteins and hormones, and reduced adipocyte size. In this study, mice performed a swimming exercise for 10 min/day, 5 days/week for 3 weeks. We then investigated the effect of this exercise regimen on inflammation induced by infection with drug-resistant Staphylococcus aureus strain 3089 (DRSA). In humans, DRSA causes dermatitis and pneumonitis. Similarly, DRSA induced inflammatory pneumonitis in both no-exercise (No-EX) and swim-trained (SW-EX) ICR mice. Regular exercise increased levels of the pro-inflammatory cytokines TNF-α and IL-1β and nitric oxide in both serum and whole lung tissue in SW-EX, as compared to No-EX control mice. Moreover, levels of the antimicrobial peptide cathelicidin were significantly increased in visceral adipose tissue and whole lung tissue in the SW-EX group, and this was accompanied by a reduction in the size of visceral adipocytes. In addition, levels of the inflammation marker peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1) were not increased in the lung tissue of SW-EX mice. These findings suggest that in these model mice, regular exercise strengthens immune system responses, potentially preventing or mitigating infectious disease.

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“…Two groups used only the 18s gene [13,28], five used only Actb gene [29][30][31][32][33]. Other authors used the Cyclophilin gene [15,31] and eight authors used Gapdh as a reference gene in their studies [12,14,[34][35][36][37][38][39][40]. Only three studies used more than one gene as internal control: Gapdh and large ribosomal protein P0 (RPLP0) genes [41]; Gapdh, Actb, Hprt1 and Cyclophilin gene [42]; the other authors used Ubiquitin C gene as reference gene [43].…”

Section: Genorm Analysismentioning

confidence: 99%

Validation of Reference Genes for qPCR Analysis of Resistance Training and Androgenic Anabolic Steroids on Hypothalamus, Adrenal Gland and Fat Tissue

Pozzi¹,

Le²,

Fern³

et al. 2016

J Steroids Horm Sci

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Background: Real-time quantitative Polymerase Chain Reaction (qPCR) is a technique used for quantification of gene expression and the use of reference genes is very important to normalize the quantification results. Aim:To validate the most suitable reference genes for resistance exercise training (REx) and use of nandrolone decanoate (DECA) in three different rat tissues. Methods:A total of 40 adult male Wistar rats were distributed into four groups: exposed to vehicle three times per week (wk) (CT); eight wk of REx exposed to vehicle three times per wk (T); exposed to DECA three times per wk (D); eight wk of REx exposed to DECA three times per wk (TD). Stability of the following genes was evaluated: beta actin (Actb), alpha Tubulin (Tubulin), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Hypoxanthine phosphoribosyltransferase-1 (Hprt1) and 18s Ribossomal RNA (18s) in hypothalamus, adrenal gland and mesenteric fat tissue using GeNorm, NormFinder and BestKeeper software. Results:In hypothalamus and adrenal, all genes were suitable and none was rejected by statistical analysis; however, in fat tissue, Actb, Gapdh and Hprt1 genes were rejected by geNorm but not the others two software. Conclusion:In hypothalamus and adrenal all selected genes analized were stable and can be used for qPCR gene expression analysis. However, in fat tissue we suggest the Tubulin gene as most stable gene.

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Role of physical exercise on hepatic insulin, glucocorticoid and inflammatory signaling pathways in an animal model of non-alcoholic steatohepatitis

Cited by 22 publications

References 64 publications

Influence of Acute and Chronic Exercise on Glucose Uptake

Influence of Acute and Chronic Exercise on Glucose Uptake

Effect of Regular Exercise on Inflammation Induced by Drug-resistant Staphylococcus aureus 3089 in ICR mice

Validation of Reference Genes for qPCR Analysis of Resistance Training and Androgenic Anabolic Steroids on Hypothalamus, Adrenal Gland and Fat Tissue

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