Role of Phote-HrTH (Phormia terraenovae hypertrehalosemic hormone) in modulating the supercontractile muscles of the crop of adult Phormia regina Meigen

Stoffolano, John G.; Croke, Katherine; Chambers, James J.; GÄde, Gerd; Solari, Paolo; Liscia, Anna Maria

doi:10.1016/j.jinsphys.2014.10.014

Cited by 17 publications

(17 citation statements)

References 46 publications

(61 reference statements)

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“…At the same time, we tested the outcome of AKH treatment on crop activity according to its previously reported effect in P . regina [44]. Moreover, on the basis of our results, and of data in the literature, a possible mechanism regulating this modulation is discussed.…”

Section: Introductionmentioning

confidence: 52%

Opposite effects of 5-HT/AKH and octopamine on the crop contractions in adult Drosophila melanogaster: Evidence of a double brain-gut serotonergic circuitry

et al. 2017

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This study showed that in adult Drosophila melanogaster, the type of sugar—either present within the crop lumen or in the bathing solution of the crop—had no effect on crop muscle contraction. What is important, however, is the volume within the crop lumen. Electrophysiological recordings demonstrated that exogenous applications of serotonin on crop muscles increases both the amplitude and the frequency of crop contraction rate, while adipokinetic hormone mainly enhances the crop contraction frequency. Conversely, octopamine virtually silenced the overall crop activity. The present study reports for the first time an analysis of serotonin effects along the gut-brain axis in adult D. melanogaster. Injection of serotonin into the brain between the interocellar area shows that brain applications of serotonin decrease the frequency of crop activity. Based on our results, we propose that there are two different, opposite pathways for crop motility control governed by serotonin: excitatory when added in the abdomen (i.e., directly bathing the crop) and inhibitory when supplied within the brain (i.e., by injection). Finally, our results point to a double brain-gut serotonergic circuitry suggesting that not only the brain can affect gut functions, but the gut can also affect the central nervous system. On the basis of our results, and data in the literature, a possible mechanism for these two discrete serotonergic functions is suggested.

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Section: Introductionmentioning

confidence: 52%

Opposite effects of 5-HT/AKH and octopamine on the crop contractions in adult Drosophila melanogaster: Evidence of a double brain-gut serotonergic circuitry

et al. 2017

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“…A similar stomatogastric nervous system is present in the tsetse fly (Langley, 1965), where AKH neuropeptides may be released from axon termini and regulate feeding, digestion or nutrient transport. Previous reports indicated a hormonal role for insect AKH in the regulation of midgut, salivary gland, and crop functioning (Bil et al, 2014;Kodrík et al, 2012;Vinokurov et al, 2014;Stoffolano et al, 2014), but it remains elusive whether neuronal projections innervating these tissues or local production of AKH is involved.…”

Section: Discussionmentioning

confidence: 99%

Characterization and pharmacological analysis of two adipokinetic hormone receptor variants of the tsetse fly, Glossina morsitans morsitans

Caers

Janssen

Rompay

et al. 2016

Insect Biochemistry and Molecular Biology

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“…Moreover, the volume of liquid within the crop influences the contraction rates in both P. regina and M. domestica (Holling, 1976; Stoffolano et al 2014b). Neuropeptides, such as adipokinetic hormone (AKH), dromyosuppressin (DMS), drosulfakinin, and FMRFamide, and biogenic amines, such as serotonin (5-hydroxytryptamine, 5-HT) and octopamine, modulate crop contraction rates in P. regina and/or D. melanogaster (Duttlinger et al 2002; Liscia et al 2012; Palmer et al 2007; Solari et al 2017; Stoffolano et al 2013; Stoffolano et al 2014a). Notably, nerves associated with the crop contain DMS, FMRFamide, insulin-like peptide, 5-HT, and AKH, suggesting the potential for direct neural control of crop contractions (Cao and Brown, 2001; Duttlinger et al 2002; Haselton et al 2004; Lee and Park, 2004; Liu et al 2011; McCormick and Nichols, 1993).…”

Section: Introductionmentioning

confidence: 99%

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

Calkins

DeLaat

Piermarini

2017

Journal of Insect Physiology

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In adult dipteran insects (flies), the crop is a diverticulum of the esophagus that serves as a food storage organ. The crop pumps stored contents into the alimentary canal for digestion and absorption. The pumping is mediated by peristaltic contractions of the crop musculature. In adult female mosquitoes, the crop (ventral diverticulum) selectively stores sugar solutions (e.g., nectar); proteinaceous blood meals by-pass the crop and are transferred directly to the midgut for digestion. The mechanisms that regulate crop contractions have never been investigated in mosquitoes. Here we provide the first physiological characterization of the contractile properties of the mosquito crop and explore the mechanisms that regulate crop contractions. Using an in vitro bioassay we found that the isolated crop spontaneously contracts in Ringer solution for at least 1 hour and its contractions are dependent on extracellular Ca2+. Adding serotonin (5- hydroxytryptamine, 5-HT) or a membrane-permeable analog of cyclic adenosine monophosphate (cAMP) to the extracellular bath increased the frequency of crop contractions. On the other hand, adding benzethonium chloride (BzCl; a chemical that mimics the effects of myosuppressins), H-89 or Rp-cAMPS (inhibitors of protein kinase A, PKA), or carbenoxolone (an inhibitor of gap junctions) reduced the frequency of the unstimulated, spontaneous and/or 5-HT-stimulated crop contractions. Adding aedeskinin III did not detectably alter crop contraction rates. In addition to pharmacological evidence of gap junctions, we demonstrated that the crop expressed several mRNAs encoding gap junctional proteins (i.e. innexins). Furthermore, we localized immunoreactivity for innexin 2 and innexin 3 to muscle and epithelial cells of the crop, respectively. Our results 1) suggest that 5-HT and myosupressins oppositely regulate contractile activity of the mosquito crop, and 2) provide the first evidence for putative roles of cAMP, PKA, and gap junctions in modulating contractile activity of the dipteran crop.

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Role of Phote-HrTH (Phormia terraenovae hypertrehalosemic hormone) in modulating the supercontractile muscles of the crop of adult Phormia regina Meigen

Cited by 17 publications

References 46 publications

Opposite effects of 5-HT/AKH and octopamine on the crop contractions in adult Drosophila melanogaster: Evidence of a double brain-gut serotonergic circuitry

Opposite effects of 5-HT/AKH and octopamine on the crop contractions in adult Drosophila melanogaster: Evidence of a double brain-gut serotonergic circuitry

Characterization and pharmacological analysis of two adipokinetic hormone receptor variants of the tsetse fly, Glossina morsitans morsitans

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

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