Role of Phospholipid Head Groups in Ethanol Tolerance of Saccharomyces cerevisiae

Mishra, Prashant; Prasad, Rajendra

doi:10.1099/00221287-134-12-3205

Cited by 30 publications

(37 citation statements)

References 3 publications

(5 reference statements)

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“…4), which appear to be related to the known cellular effects of ethanol. For example, ethanol influences cell membrane integrity (Ingram and Buttke, 1984), and ethanol tolerance relies on the phospholipid composition of the cell membrane (Mishra and Prasad, 1988). Phospholipid transporters enable directed movements of phospholipids and thus may be harmful under high ethanol concentrations.…”

Section: Resultsmentioning

confidence: 99%

The Genomic Landscape and Evolutionary Resolution of Antagonistic Pleiotropy in Yeast

et al. 2012

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SUMMARY Antagonistic pleiotropy (AP) or genetic tradeoff is an important concept invoked frequently in theories of aging, cancer, genetic disease, and other common phenomena. But, it is unclear how prevalent AP is, which genes are subject to AP, and to what extent and how AP may be resolved. By measuring the fitness difference between the wild-type and null alleles of ~5000 nonessential genes in yeast, we find that, in any given environment, yeast expresses hundreds of genes that harm rather than benefit the organism, demonstrating widespread AP. Nonetheless, under sufficient selection, AP is often resolvable through regulatory evolution, primarily by trans-acting changes, although in one case we also detect a cis-acting change and localize its causal mutation. AP resolution, however, is slower in smaller populations, predicting more unresolved AP in multicellular organisms than in yeast. These findings provide the empirical foundation for AP-dependent theories and have broad biomedical and evolutionary implications.

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Section: Resultsmentioning

confidence: 99%

The Genomic Landscape and Evolutionary Resolution of Antagonistic Pleiotropy in Yeast

et al. 2012

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“…Mishra and Prasad (55, 56) similarly exposed exponentialgrowth-phase S. cerevisiae to a buffered (pH 4.5) 2 M (7.3 wt%) ethanol solution to ascertain ethanol tolerance via L-alanine uptake and proton flux through the membrane. Based on the conclusions of these studies, they proposed that increased levels of phosphatidylserine promoted a favorable anion/zwitterion ratio in the plasma membrane that increased ethanol tolerance (55). They also reported that cells with membranes enriched in unsaturated fatty acids exhibited higher ethanol tolerance based upon their L-alanine uptake, proton efflux, and fermentation rate (56).…”

Section: The Effects Of Ethanol On Yeast Cell Membranesmentioning

confidence: 99%

Examining the Role of Membrane Lipid Composition in Determining the Ethanol Tolerance of Saccharomyces cerevisiae

Henderson

Block

2014

Appl Environ Microbiol

126

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Yeast (Saccharomyces cerevisiae) has an innate ability to withstand high levels of ethanol that would prove lethal to or severely impair the physiology of other organisms. Significant efforts have been undertaken to elucidate the biochemical and biophysical mechanisms of how ethanol interacts with lipid bilayers and cellular membranes. This research has implicated the yeast cellular membrane as the primary target of the toxic effects of ethanol. Analysis of model membrane systems exposed to ethanol has demonstrated ethanol's perturbing effect on lipid bilayers, and altering the lipid composition of these model bilayers can mitigate the effect of ethanol. In addition, cell membrane composition has been correlated with the ethanol tolerance of yeast cells. However, the physical phenomena behind this correlation are likely to be complex. Previous work based on often divergent experimental conditions and time-consuming low-resolution methodologies that limit large-scale analysis of yeast fermentations has fallen short of revealing shared mechanisms of alcohol tolerance in Saccharomyces cerevisiae. Lipidomics, a modern mass spectrometry-based approach to analyze the complex physiological regulation of lipid composition in yeast and other organisms, has helped to uncover potential mechanisms for alcohol tolerance in yeast. Recent experimental work utilizing lipidomics methodologies has provided a more detailed molecular picture of the relationship between lipid composition and ethanol tolerance. While it has become clear that the yeast cell membrane composition affects its ability to tolerate ethanol, the molecular mechanisms of yeast alcohol tolerance remain to be elucidated.

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“…Ethanol, for example, affects transmembrane proton flow in S. cerevisiae (Cartwright et al, 1986(Cartwright et al, , 1987Mishra & Prasad, 1988), an effect attributed either to stimulation of the passive proton influx by non-specific increase in plasma membrane permeability (Leiio & van Uden, 1984;Salgueiro et al, 1989) or to the inhibition of plasma membrane ATPase as observed in vitro (CartWright e? al., 1987).…”

Section: -6293mentioning

confidence: 99%

Activation of plasma membrane ATPase of Saccharomyces cerevisiae by octanoic acid

Viegas

Sá‐Correia²

1991

Journal of General Microbiology

143

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Plasma membrane ATPase activity of Saccharomyces cerettisiae IGC 3507111 grown in the presence of the lipophilic acid octanoic acid I450 mg I-' (0.03435 mM), pH 4.01 was 1-5-fold higher than that in cells grown in its absence. The K,,, for ATP, the pH profile and the sensitivity to orthovanadate of the basal and the activated forms of the membrane ATPase were identical. This activation was closely associated with a decrease in the biomass yield and an increase in the ethanol yield, and was rapidly reversed in uino after removal of the acid. However, the activated level was preserved when membranes were extracted and subjected to manipulations which eliminated or decreased octanoic acid incorporation in the plasma membrane. The activity of the basal plasma membrane ATPase in the total membrane fraction was slightly increased by incubation at pH6.5 with octanoic acid at 100 mg I-' or less (2.4 mg acid form plus 97.6 mg octanoate ion 1-l). However, destruction of the permeability barrier between the enzyme and its substrate could not explain the in tlitfo activation. A role for plasma membrane ATPase activation in the regulation of the intracellular pH (pHi) of cells grown with octanoic acid was not proven.

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Role of Phospholipid Head Groups in Ethanol Tolerance of Saccharomyces cerevisiae

Cited by 30 publications

References 3 publications

The Genomic Landscape and Evolutionary Resolution of Antagonistic Pleiotropy in Yeast

The Genomic Landscape and Evolutionary Resolution of Antagonistic Pleiotropy in Yeast

Examining the Role of Membrane Lipid Composition in Determining the Ethanol Tolerance of Saccharomyces cerevisiae

Activation of plasma membrane ATPase of Saccharomyces cerevisiae by octanoic acid

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