Role of phospho<i>enol</i>pyruvate carboxylase in organic acid accumulation during peach fruit development

Moing, Annick; Rothan, Christophe; Svanella, Laurence; Just, Daniel; Diakou, Paraskevi; Raymond, Philippe; Gaudillère, J.P.; Monet, R.

doi:10.1034/j.1399-3054.2000.108001001.x

Cited by 63 publications

(33 citation statements)

References 46 publications

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“…These molecular data mirror the fact that berries can form malate from 14 CO 2 at any stage of development [90] and that enzymes involved in MA synthesis are not systematically down-regulated during ripening when no more net accumulation of MA occurs. This observation is in accordance with the literature where no relationship between MA content and the activities of PEPC or malic enzyme were observed in low and high acid peach cultivars [91], the acidless grape mutant Gora Chirine [92], apple [93], [94] and in low and high acidic loquat cultivars [95]. It therefore seems unlikely that MA accumulation is determined by the activity of these pathways.…”

Section: Resultssupporting

confidence: 92%

Is Transcriptomic Regulation of Berry Development More Important at Night than During the Day?

et al. 2014

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Diurnal changes in gene expression occur in all living organisms and have been studied on model plants such as Arabidopsis thaliana. To our knowledge the impact of the nycthemeral cycle on the genetic program of fleshly fruit development has been hitherto overlooked. In order to circumvent environmental changes throughout fruit development, young and ripening berries were sampled simultaneously on continuously flowering microvines acclimated to controlled circadian light and temperature changes. Gene expression profiles along fruit development were monitored during both day and night with whole genome microarrays (Nimblegen® vitis 12x), yielding a total number of 9273 developmentally modulated probesets. All day-detected transcripts were modulated at night, whereas 1843 genes were night-specific. Very similar developmental patterns of gene expression were observed using independent hierarchical clustering of day and night data, whereas functional categories of allocated transcripts varied according to time of day. Many transcripts within pathways, known to be up-regulated during ripening, in particular those linked to secondary metabolism exhibited a clearer developmental regulation at night than during the day. Functional enrichment analysis also indicated that diurnally modulated genes considerably varied during fruit development, with a shift from cellular organization and photosynthesis in green berries to secondary metabolism and stress-related genes in ripening berries. These results reveal critical changes in gene expression during night development that differ from daytime development, which have not been observed in other transcriptomic studies on fruit development thus far.

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Section: Resultssupporting

confidence: 92%

Is Transcriptomic Regulation of Berry Development More Important at Night than During the Day?

et al. 2014

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“…Fantasia and Jalousia peach ( Prunus persica (L.) Batsch) cultivars grafted on Rubira ( P. persica ) rootstock were grown in a greenhouse as described previously (Moing et al. 2000).…”

Section: Methodsmentioning

confidence: 99%

“…For RNA extraction, each fruit sample was obtained from pooled mesocarp plus epidermis of 4 fruits. Total RNA was extracted from fruit mesocarp plus epidermis, young leaves and flower buds following the method described previously (Moing et al. 2000) with modifications.…”

Section: Methodsmentioning

confidence: 99%

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Isolation and characterization of six peach cDNAs encoding key proteins in organic acid metabolism and solute accumulation: involvement in regulating peach fruit acidity

Etienne

Moing

Dirlewanger

et al. 2002

Physiologia Plantarum

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114

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As in many other fleshy fruits, the predominant organic acids in ripe peach (Prunus persica (L.) Batsch) fruit are malic and citric acids. The accumulation of these metabolites in fruit flesh is regulated during fruit development. Six peach fruit-related genes implicated in organic acid metabolism (mitochondrial citrate synthase; cytosolic NAD-dependent malate dehydrogenase, and cytosolic NADP-dependent isocitrate dehydrogenase) and storage (vacuolar proton translocating pumps: one vacuolar H+-ATPase, and two vacuolar H+-pyrophosphatases) were cloned. Five of these peach genes were homologous to genes isolated from fruit in other fleshy fruit species. Phylogenetic and expression analyses suggested the existence of a particular vacuolar pyrophosphatase highly expressed in fruit. The sixth gene was the first cytosolic NAD-dependent malate dehydrogenase gene isolated from fruit. Gene expression was studied during the fruit development of two peach cultivars, a normal-acid (Fantasia) and a low-acid (Jalousia) cultivar. The overall expression patterns of the organic acid-related genes appeared strikingly similar for the two cultivars. The genes involved in organic acid metabolism showed a stronger expression in ripening fruit than during the earlier phases of development, but their expression patterns were not necessarily correlated with the changes in organic acid contents. The tonoplast proton pumps showed a biphasic expression pattern more consistent with the patterns of organic acid accumulation, and the tonoplast pyrophosphatases were more highly expressed in the fruit of the low-acid cultivar during the second rapid growth phase of the fruit.

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“…That may mean a faster and shorter term of citrate accumulation in some sweet oranges. Usually, a constant decrease in organic acid is found in most fruits due to its transportation and degradation (Diakou et al , Moing et al , Zhao et al ), however, a slight increase in citrate in the late fruit‐development stage (from 110 to 160 DAFB) was detected in ‘Dahongtiancheng’ and ‘Bingtangcheng’ (Fig. ).…”

Section: Discussionmentioning

confidence: 98%

Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis)

XiongJun

et al. 2016

Physiologia Plantarum

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Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using 'Dahongtiancheng' (Citrus sinensis) and 'Bingtangcheng' (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'Dahongtiancheng' and 'Bingtangcheng' exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50-70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670-2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in 'Dahongtiancheng'. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of 'Dahongtiancheng'. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange.

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Role of phosphoenolpyruvate carboxylase in organic acid accumulation during peach fruit development

Cited by 63 publications

References 46 publications

Is Transcriptomic Regulation of Berry Development More Important at Night than During the Day?

Is Transcriptomic Regulation of Berry Development More Important at Night than During the Day?

Isolation and characterization of six peach cDNAs encoding key proteins in organic acid metabolism and solute accumulation: involvement in regulating peach fruit acidity

Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis)

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