Role of peroxisome proliferator‐activated receptor alpha in the expression of hepatic fatty acid oxidation‐related genes in chickens

Honda, Kazuhisa; Saneyasu, Takaoki; Sugimoto, Haruka; Kurachi, Kiyotaka; Takagi, Shoko; Kamisoyama, Hiroshi

doi:10.1111/asj.12392

Cited by 11 publications

(7 citation statements)

References 28 publications

(39 reference statements)

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“…Although there are fewer studies investigating the links between PPAR and beta oxidation in chicken, there is indication that there are some similarities to other model organisms. In particular, carnitine palmitoyltransferase 1a ( CPT1A ) transcription seems to be regulated through PPARα (Honda et al 2016 ). Similar studies of PPAR-related effects of PFOS on the embryonic chicken liver have given varied responses, with studies in both 18 and 21 days chicken embryos indicating no significant changes to expression of PPAR-induced genes (O'Brien et al 2009 ; Strömqvist et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of perfluorooctane sulfonate on genes controlling hepatic fatty acid metabolism in livers of chicken embryos

Jacobsen

Nordén

Engwall

et al. 2018

Environ Sci Pollut Res

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Per- and polyfluoroalkyl substances (PFAS) are synthetic surfactants with a wide variety of applications; however, due to their stability, they are particularly resistant to degradation and, as such, are classed as persistent organic pollutants. Perfluorooctane sulfonate (PFOS) is one such PFAS that is still detectable in a range of different environmental settings, despite its use now being regulated in numerous countries. Elevated levels of PFOS have been detected in various avian species, and the impact of this on avian health is of interest when determining acceptable levels of PFOS in the environment. Due to its similarities to naturally occurring fatty acids, PFOS has potential to disrupt a range of biological pathways, particularly those associated with lipid metabolism, and this has been shown in various species. In this study, we have investigated how in ovo exposure to environmentally relevant levels of PFOS affects expression of genes involved in lipid metabolism of developing chicken embryos. We have found a broad suppression of transcription of genes involved in fatty acid oxidation and PPAR-mediated transcription with more significant effects apparent at lower doses of PFOS. These results highlight the need for more research investigating the biological impacts of low levels of PFAS to properly inform environmental policy governing their regulation.Electronic supplementary materialThe online version of this article (10.1007/s11356-018-2358-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Effects of perfluorooctane sulfonate on genes controlling hepatic fatty acid metabolism in livers of chicken embryos

Jacobsen

Nordén

Engwall

et al. 2018

Environ Sci Pollut Res

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“…5A ). PPARα is a key transcription factor in promoting fatty acid betaoxidation [ 16 ]. To pursue this idea, we assessed mRNA expression involved in beta-oxidation including peroxisomal acyl-coenzyme A oxidase 1 (ACOX1), acyl-coenzyme A dehydrogenase for mediumchain fatty acids (AcadM), and acetyl-CoA carboxylase2 (ACC2), as well as mRNAs associated with TG degradation such as hormonesensitive lipase (HSL) and monoacylglycerol lipase (MGLL), and mRNAs that transport FFA into mitochondria such as carnitine palmitoyltransferase1 (CPT1) and CPT2 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Improved systemic metabolism and adipocyte biology in miR-150 knockout mice

Kang

Liu

et al. 2018

Metabolism

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Introduction: Short non-coding micro-RNAs (miRNAs) are post-transcriptional factors that directly regulate protein expression by degrading or inhibiting target mRNAs; however, the role of miRNAs in obesity and cardiometabolic disease remains unclarified. Based on our earlier study demonstrating that miR-150 influences lipid metabolism, we have studied effects of miR-150 on systemic metabolism and adipocyte biology. Materials and Methods: Metabolic phenotypes including body weight, food intake, body composition, glucose tolerance and insulin sensitivity were assessed in WT and global miR-150 KO male mice fed a high-fat diet. Molecular changes in epididymal adipose tissue were evaluated through qRT-PCR and Western blotting. Results: miR-150 KO mice displayed lower body weight characterized by a reduction in % fat mass while % lean mass was increased. Lower body weight was associated with reduced food consumption and an increase in circulating leptin concentrations, as well as enhanced insulin sensitivity and glucose tolerance compared with WT mice. Absence of miR-150 resulted in increased mTOR expression known to participate in increased leptin production leading to reduction of food intake. Expression of PGC-1α, another target gene of miR-150, was also increased together with upregulation of PPARα and glycerol kinase in adipose tissue as well as other genes participating in triglyceride degradation and lipid oxidation. Conclusion: miR-150 KO mice showed metabolic benefits accompanied by reduced body weight, decreased energy intake, and enhanced lipid metabolism. miR-150 may represent both a biomarker and novel therapeutic target regarding obesity and insulin resistance.

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“…Our data revealed that activation of PPARα inhibited the expression of FASN and ACC, and lipid formation and cholesterol synthesis, which suggested that changes in lipid accumulation are caused by activity of PPARα through the regulation of FASN and ACC. Previous research studies showed that the PPARα agonist WY14643 effectively enhanced the expression of CPT-1α and ACO genes in chicken hepatoma cells, leading to the activation of the fatty acid oxidative pathway . Additionally, leucine stimulated milk synthesis in BMECs via PI3K-DDX59 signaling .…”

Section: Discussionmentioning

confidence: 99%

Enhancement of PPARα-Inhibited Leucine Metabolism-Stimulated β-Casein Synthesis and Fatty Acid Synthesis in Primary Bovine Mammary Epithelial Cells

Huang,

Li,

Ning

et al. 2023

J. Agric. Food Chem.

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Leucine, a kind of branched-chain amino acid, plays a regulatory role in the milk production of mammalian mammary glands, but its regulatory functions and underlying molecular mechanisms remain unknown. This work showed that a leucineenriched mixture (LEUem) supplementation increased the levels of milk protein and milk fat synthesis in primary bovine mammary epithelial cells (BMECs). RNA-seq of leucine-treated BMECs indicated alterations in lipid metabolism, translation, ribosomal structure and biogenesis, and inflammatory response signaling pathways. Meanwhile, the supplementation of leucine resulted in mTOR activation and increased the expression of BCKDHA, FASN, ACC, and SCD1. Interestingly, the expression of PPARα was independently correlated with the leucine-supplemented dose. PPARα activated by WY-14643 caused significant suppression of lipogenic genes expression. Furthermore, WY-14643 attenuated leucine-induced β-casein synthesis and enhanced the level of BCKDHA expression. Moreover, promoter analysis revealed a peroxisome-proliferator-response element (PPRE) site in the bovine BCKDHA promoter, and WY-14643 promoted the recruitment of PPARα onto the BCKDHA promoter. Together, the present data indicate that leucine promotes the synthesis of β-casein and fatty acid and that PPARα-involved leucine catabolism is the key target.

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Role of peroxisome proliferator‐activated receptor alpha in the expression of hepatic fatty acid oxidation‐related genes in chickens

Cited by 11 publications

References 28 publications

Effects of perfluorooctane sulfonate on genes controlling hepatic fatty acid metabolism in livers of chicken embryos

Effects of perfluorooctane sulfonate on genes controlling hepatic fatty acid metabolism in livers of chicken embryos

Improved systemic metabolism and adipocyte biology in miR-150 knockout mice

Enhancement of PPARα-Inhibited Leucine Metabolism-Stimulated β-Casein Synthesis and Fatty Acid Synthesis in Primary Bovine Mammary Epithelial Cells

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