Role of peripheral polyamines in the development of inflammatory pain

Silva, Mariane Alves; Klafke, Jonatas Zeni; Rossato, Mateus; Gewehr, Camila de Campos Velho; Guerra, Gustavo Petri; Rubin, Maribel Antonello; Ferreira, Juliano

doi:10.1016/j.bcp.2011.04.015

Cited by 21 publications

(16 citation statements)

References 32 publications

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“…DFMO (0.27–27 nmol/site) was given 1 hour prior training. Doses used were based on previous work [16], [17], [18] and dose-effect curves (Figure 1B, 1D, 1E). Figure 1A, 2A and 2B display a time-line with treatments and administration time.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of the Polyamine System Counteracts β-Amyloid Peptide-Induced Memory Impairment in Mice: Involvement of Extrasynaptic NMDA Receptors

et al. 2014

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In Alzheimer's disease (AD), the β-amyloid peptide (Aβ) has been causally linked to synaptic dysfunction and cognitive impairment. Several studies have shown that N-Methyl-D-Aspartate receptors (NMDAR) activation is involved in the detrimental actions of Aβ. Polyamines, like spermidine and spermine, are positive modulators of NMDAR function and it has been shown that their levels are regulated by Aβ. In this study we show here that interruption of NMDAR modulation by polyamines through blockade of its binding site at NMDAR by arcaine (0.02 nmol/site), or inhibition of polyamine synthesis by DFMO (2.7 nmol/site), reverses Aβ25–35-induced memory impairment in mice in a novel object recognition task. Incubation of hippocampal cell cultures with Aβ25–35 (10 µM) significantly increased the nuclear accumulation of Jacob, which is a hallmark of NMDAR activation. The Aβ-induced nuclear translocation of Jacob was blocked upon application of traxoprodil (4 nM), arcaine (4 µM) or DFMO (5 µM), suggesting that activation of the polyamine binding site at NMDAR located probably at extrasynaptic sites might underlie the cognitive deficits of Aβ25–35-treated mice. Extrasynaptic NMDAR activation in primary neurons results in a stripping of synaptic contacts and simplification of neuronal cytoarchitecture. Aβ25–35 application in hippocampal primary cell cultures reduced dendritic spine density and induced alterations on spine morphology. Application of traxoprodil (4 nM), arcaine (4 µM) or DFMO (5 µM) reversed these effects of Aβ25–35. Taken together these data provide evidence that polyamine modulation of extrasynaptic NMDAR signaling might be involved in Aβ pathology.

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Section: Methodsmentioning

confidence: 99%

Inhibition of the Polyamine System Counteracts β-Amyloid Peptide-Induced Memory Impairment in Mice: Involvement of Extrasynaptic NMDA Receptors

et al. 2014

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“…A recent study showed a polyamine‐deficient diet resulted in a rapid reduction of high intensity pain in acute pain . In addition, subcutaneous injections of spermidine or spermine triggered mechanical allodynia and oedema (rat model) . In contrast, co‐administration of fentanyl and spermine vastly increases the analgesic efficacy (15×) of fentanyl .…”

Section: Natural Compoundsmentioning

confidence: 99%

Cell‐based therapies and natural compounds for pain

Shparberg

Vickers

2018

Aust Endodontic J

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Cell-based therapies and natural compounds are increasingly being acknowledged for their potential roles in managing a variety of conditions, including pain. Stem cells have gained attention over the years for their contribution to our understanding of normal and abnormal physiology, their capacity to assist in efficient drug design and screening and their role in regenerative medicine. This chapter will review our current understanding of the complex pathophysiology of pain using adult, embryonic and induced pluripotent stem cells as models. In addition, this chapter outlines several natural compounds that are used in the treatment of neuropathic pain, with a focus on evidence-based information of the polyphenols, polyamines and endocannabinoids derived from plants and food sources. Advantages of these compounds are the higher margins of safety, low incidence of side effects. Recent evidence shows several polyphenols can induce differentiation of healthy stem cells yet inhibit cancer stem cells.

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“…The measurement of mechanical paw withdrawal threshold was carried out using von Frey filaments, according to the up-and-down paradigm as described previously (Chaplan et al, 1994;Silva et al, 2011). Briefly, rats were first acclimatized (30 min) in individual clear Plexiglas boxes (9 Â 7 Â 11 cm) on an elevated wire mesh platform to allow the access to the plantar surface of the hind paws.…”

Section: Assessment Of Hind Paw-withdrawal Response Induced By Thermamentioning

confidence: 99%

Involvement of the glutamatergic system in the nociception induced intrathecally for a TRPA1 agonist in rats

et al. 2012

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Role of peripheral polyamines in the development of inflammatory pain

Cited by 21 publications

References 32 publications

Inhibition of the Polyamine System Counteracts β-Amyloid Peptide-Induced Memory Impairment in Mice: Involvement of Extrasynaptic NMDA Receptors

Inhibition of the Polyamine System Counteracts β-Amyloid Peptide-Induced Memory Impairment in Mice: Involvement of Extrasynaptic NMDA Receptors

Cell‐based therapies and natural compounds for pain

Involvement of the glutamatergic system in the nociception induced intrathecally for a TRPA1 agonist in rats

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