Role of pea protein hydrolysates as antinephrotoxicity

Hidayat, Meilinah; Prahastuti, Sijani; Wargasetia, Teresa Liliana; Ferdinand, Vincentius; Wahyudianingsih, Roro; Soemardji, Andreanus Andaja; Rahmawati, Siti Farah; Suliska, Nova; Hasan, Khomaini

doi:10.4103/jrptps.jrptps_14_17

Cited by 2 publications

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“…PHGPB reduced the levels of TGF-β1 and Fibronectin in glucose-induced mesangial cell lines (Hidayat et al 2021). Provision PHGPB dose of 100 mg/kg BW/d for 28 days tends to improve the parameters of kidney function (Urea, Creatinine, Uric acid) and renal histopathology in Cisplatin-induced rats (Hidayat et al 2019). However, in that 28-day study, induction of a single dose of Cisplatin 10 mg/kg BW (Nematbakhsh et al 2013) has not caused the kidney histopathology signs of fibrosis.…”

Section: Introductionmentioning

confidence: 77%

“…The hydrolysates with small molecular weight obtained from ultrafiltration showed the highest antioxidant activities in all relevant assays (Ding and Lin 2020). The protein hydrolysate derived from the hydrolysis of Pisum sativum (green peas), whose amino acid sequence is LERGDT, has many bands with small molecular mass <10 kDa, promising good potential as an antioxidant, especially as a source of SOD (Hidayat et al 2019), and anti-inflammatory also.…”

Section: Discussionmentioning

confidence: 99%

“…The USA. Trinidad Benham Corp. Denver, Co 80237 was prepared for hydrolysis using the previously described simple hydrolysis method (Hidayat et al 2019). The dry seeds were ground into powder, after sifting through a sieve 120-mesh, Vol.…”

Section: Preparation Protein Hydrolysatementioning

confidence: 99%

“…Proteins in PHGPB were separated using sodium dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (Spectra Multicolor Low Range Protein Ladder paints. No 26628) patterns showed many bands 14.4 kDa with much molecular weight smaller than ten kDa (Hidayat et al 2019). PHGPB reduced the levels of TGF-β1 and Fibronectin in glucose-induced mesangial cell lines (Hidayat et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…However, in that 28-day study, induction of a single dose of Cisplatin 10 mg/kg BW (Nematbakhsh et al 2013) has not caused the kidney histopathology signs of fibrosis. PHGPB treatment improved the appearance of cloudy swollen tubular degeneration, nuclear necrosis, and hyaline casts (Hidayat et al 2019). It may take a longer time or a different regimen of Cisplatin to induce a histopathologic TIF condition.…”

Section: Introductionmentioning

confidence: 99%

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Protein Hydrolysate of Green Peas Bromelain Attenuates Kidney Fibrosis in Cisplatin-Induced Nephrotoxicity Rats: Emphasis on Anti-Inflammatory Activities

et al. 2022

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Massive tubule fibrosis is a histopathology hallmark of chronic renal failure (CRF). The previous study of protein hydrolysate of green pea (Pisum sativum) bromelain (PHGPB) showed promising results as an antifibrosis in glucose-induced renal mesangial culture cells, by decreasing their TGF-β levels. In this experiment the anti-inflammatory, anti-fibrotic effect of PHGPB in Cisplatin-induced chronic renal failure (CRF) rats was measured. The purpose of this study is to evaluate the anti-inflammatory and antifibrosis effect of PHGPB in Cisplatin-induced chronic renal failure rats. Five groups consisted of five rats: negative control, Cisplatin control, and three groups of Cisplatin+PHGPB (dose of 100, 200, and 400 mg/kg BW/day) treated for 56 days. The examined parameters are Beta-2-microglobulin (β2MG), hs-CRP, histopathological observations of Masson's trichrome stain, and IHC. The level of β2MG and hs-CRP in the Cisplatin+PHGPB group lowered and was highly different from Cisplatin control (p<0.05) depending on doses. In histopathology, the Cisplatin+PHGPB 400 mg/kg BW group showed less fibrosis and no significant difference with the negative control in Masson trichome staining. While in IHC histopathology, PHGPB treatment slightly ameliorated TGF-β expression and intensity of TGF-β values convincingly. In conclusion, PHGPB can relieve kidney fibrosis in cisplatin-induced nephrotoxicity rats: Emphasis on anti-inflammatory activities.

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Protein Hydrolysatementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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