Role of pathogen-induced volatiles in the Nicotiana tabacum–Golovinomyces cichoracearum interaction

Quaglia, Mara; Fabrizi, Mario; Zazzerini, A.; Zadra, Claudia

doi:10.1016/j.plaphy.2011.11.006

Cited by 24 publications

(16 citation statements)

References 83 publications

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“…To date, the role of terpenoid compounds in the R. fascians -plant interactions remains unknown, although some tobacco-pathogen interactions are known for leading to alterations in the terpenes pathway. The induction of the monoterpene E-( b )-ocimene and the sesquiterpenes caryophyllene, β-elemene and α-farnesene, have been shown during the interaction of Nicotiana tabacum with Golovinomyces cichoracearum [25] and Pseudomonas syringae [26], respectively. Seo [27] identified a diterpene, the (11E,13E)-labda-11,13-diene-8α,15-diol, as an endogenous signal for the activation of tobacco defense responses to wounding and to infection with the tobacco mosaic virus; many terpenoids are known for their function as phytoalexins, contributing to plant defenses against microbes [28].…”

Section: Resultsmentioning

confidence: 99%

Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen Rhodococcus fascians, as a Potential Source of Bioactive Compounds

Nacoulma

Vandeputte

Lorenzi

et al. 2013

IJMS

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Leafy gall is a plant hyperplasia induced upon Rhodococcus fascians infection. Previously, by genomic and transcriptomic analysis, it has been reported that, at the early stage of symptom development, both primary and secondary metabolisms are modified. The present study is based on the hypothesis that fully developed leafy gall, could represent a potential source of new bioactive compounds. Therefore, non-targeted metabolomic analysis of aqueous and chloroform extracts of leafy gall and non-infected tobacco was carried out by 1H-NMR coupled to principal component analysis (PCA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA). Polar metabolite profiling reflects modifications mainly in the primary metabolites and in some polyphenolics. In contrast, main modifications occurring in non-polar metabolites concern secondary metabolites, and gas chromatography and mass spectrometry (GC-MS) evidenced alterations in diterpenoids family. Analysis of crude extracts of leafy galls and non-infected tobacco leaves exhibited a distinct antiproliferative activity against all four tested human cancer cell lines. A bio-guided fractionation of chloroformic crude extract yield to semi-purified fractions, which inhibited proliferation of glioblastoma U373 cells with IC50 between 14.0 and 2.4 μg/mL. Discussion is focused on the consequence of these metabolic changes, with respect to plant defense mechanisms following infection. Considering the promising role of diterpenoid family as bioactive compounds, leafy gall may rather be a propitious source for drug discovery.

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Section: Resultsmentioning

confidence: 99%

Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen Rhodococcus fascians, as a Potential Source of Bioactive Compounds

Nacoulma

Vandeputte

Lorenzi

et al. 2013

IJMS

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“…The essential oil of Cingapura cultivar was dominated by sesquiterpenes hydrocarbons (61.8–86.1%), as δ-elemene (57.6%) and β-caryophyllene (3.8%), in contrast with a specimen collected in Cameroon that showed low concentrations of δ-elemene (2.6%) and β-caryophyllene (7.3%) [ 21 ]. Inoculated plants activate signal transduction cascades mediated by plant hormones, which lead to higher expression of genes related to defense [ 27 ]. There are many chemical compounds involved in interactions between plants, which include aldehydes and low molecular weight alcohols such as 2 E -hexenal, 3 Z -hexen-1-ol, terpenes such as myrcene and ocimene consisting of mixtures of E -β-ocimene, Z -β-ocimene and allo -ocimene [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Secondary Metabolic Profiles of Two Cultivars of Piper nigrum (Black Pepper) Resulting from Infection by Fusarium solani f. sp. piperis

Luz

Yamaguchi

Kato

et al. 2017

IJMS

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Bragantina and Cingapura are the main black pepper (Piper nigrum L.) cultivars and the Pará state is the largest producer in Brazil with about 90% of national production, representing the third largest production in the world. The infection of Fusarium solani f. sp. piperis, the causal agent of Fusarium disease in black pepper, was monitored on the cultivars Bragantina (susceptible) and Cingapura (tolerant), during 45 days’ post infection (dpi). Gas Chromatography-Mass spectrometry (GC-MS) analysis of the volatile concentrates of both cultivars showed that the Bragantina responded with the production of higher contents of α-bisabolol at 21 dpi and a decrease of elemol, mostly at 30 dpi; while Cingapura displayed an decrease of δ-elemene production, except at 15 dpi. The phenolic content determined by the Folin Ciocalteu method showed an increase in the leaves of plants inoculated at 7 dpi (Bragantina) and 7–15 dpi (Cingapura); in the roots, the infection caused a phenolic content decrease in Bragantina cultivar at 45 dpi and an increase in the Cingapura cultivar at 15, 30 and 45 dpi. High Performance Liquid Chromatography-Mass spectrometry (HPLC-MS) analysis of the root extracts showed a qualitative variation of alkamides during infection. The results indicated that there is a possible relationship between secondary metabolites and tolerance against phytopathogens.

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“…The lipoxygenase product can act either as an intermediate or as an end product of a metabolic route. In plants, lipoxygenases are involved in the biosynthesis of jasmonic acid and aldehyde that are responsible for defense mechanism signaling (Quaglia, Fabrizi, Zazzerini, & Zadra, 2012;Senthilraja, Anand, Kennedy, Raguchander, & Samiyappan, 2013).…”

Section: Methodsmentioning

confidence: 99%

<b>Effect of foliar fungicide and plant spacing on the expression of lipoxygenase enzyme and grain rot in maize hybrids

et al. 2017

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ABSTRACT. This study was carried out with the objective of evaluating the effect of fungicide application on grain rot in commercial maize hybrids and the relation between grain rot and the expression of lipoxygenase enzyme in grain in conventional row spacing of 0.70 m and reduced row spacing of 0.45 m. Treatments were made in a 3 x 8 factorial scheme, using three forms of management with fungicide (Trifloxystrobin + Prothioconazole) and eight maize hybrids divided into two groups (tolerant and susceptible) with three repetitions, totaling 72 plots in each environment (conventional and reduced spacing) in the 2013/2014 crop. The following characteristics were evaluated: grain rot percentage and lipoxygenase enzyme expression (LOX) in the grain. The hybrid and the fungicide utilized influenced the grain rot percentage. Grain rot percentage was reduced by the use of the fungicide, and the highest reduction was in susceptible hybrids with two applications, V8 and V8+VT. There was higher expression of LOX enzyme in maize hybrids that belong to the group tolerant of fungi that cause grain rot .The use of the fungicide in two applications, V8 (eight leaves) and VT (tasseling), increased the intensity of the LOX enzyme, which was more evident for the reduced spacing. Efeito de fungicida foliar e espaçamento de plantas na expressão da enzima lipoxigenase e podridão de grãos em híbridos de milho RESUMO. O objetivo deste trabalho foi avaliar o efeito da aplicação de fungicida sobre a podridão de grãos em híbridos comerciais de milho e a relação entre os grãos ardidos e a expressão da enzima lipoxigenase no grão, em espaçamento convencional de 0,70 m e reduzido de 0,45 m. Os tratamentos foram no esquema fatorial 3 x 8, com três formas de manejo com fungicida (Trifloxistrobina + Protioconazol) e oito híbridos de milho, sendo divididos em dois grupos (tolerantes e suscetíveis), com três repetições, totalizando 72 parcelas em cada ambiente (espaçamento convencional e reduzido), na safra agrícola 2013/2014. As seguintes caractrísticas foram avaliadas: porcentagem de grãos ardidos e expressão da enzima lipoxigenase (LOX) nos grãos. O fungicida reduziu a incidência de grãos ardidos, e a maior redução ocorreu nos híbridos suscetíveis, com duas aplicações, V8 e V8+VT. Houve maior expressão da enzima LOX em híbridos de milho pertencentes ao grupo tolerante aos fungos causadores de grãos ardidos. A aplicação do fungicida em duas aplicações, V8 (oito folhas) e VT (pendoamento), aumentou a intensidade da enzima LOX, mais evidente no espaçamento reduzido.Palavras-chave: LOX, podridão de espigas, controle químico, espaçamento reduzido, Zea mays.

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Role of pathogen-induced volatiles in the Nicotiana tabacum–Golovinomyces cichoracearum interaction

Cited by 24 publications

References 83 publications

Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen Rhodococcus fascians, as a Potential Source of Bioactive Compounds

Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen Rhodococcus fascians, as a Potential Source of Bioactive Compounds

Secondary Metabolic Profiles of Two Cultivars of Piper nigrum (Black Pepper) Resulting from Infection by Fusarium solani f. sp. piperis

<b>Effect of foliar fungicide and plant spacing on the expression of lipoxygenase enzyme and grain rot in maize hybrids

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