Role of oxidative stress and inducible nitric oxide synthase in morphine-induced tolerance and dependence in mice. Effect of alpha-lipoic acid

Abdel-Zaher, Ahmed O.; Mostafa, Mostafa G. M.; Farghaly, Hanan S.M.; Hamdy, Mostafa M.; Abdel-Hady, Randa H.

doi:10.1016/j.bbr.2013.02.034

Cited by 67 publications

(47 citation statements)

References 40 publications

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“…Studies indicating a pro-oxidant activity of morphine used high doses and/or evaluated oxidative stress as a mechanism associated with tolerance and/or dependence on the opioid (7,9,11-13). Exposure to increased concentrations of morphine (as high as 6 mM) has already been used as a model of neuronal damage by oxidative stress in both in vivo and in vitro studies (including the C6 cell line) (7,11-13).…”

Section: Discussionmentioning

confidence: 99%

“…Exposure to increased concentrations of morphine (as high as 6 mM) has already been used as a model of neuronal damage by oxidative stress in both in vivo and in vitro studies (including the C6 cell line) (7,11-13). Morphine intoxication decreased the activity of antioxidant enzymes (SOD and GPx, among others) and induced apoptosis and glial activation.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, there is a growing literature supporting additional actions for morphine, as in the case of the role played by the opioid in oxidative stress (4-13). Oxidative stress is the disequilibrium between free radical generation and elimination (a free radical is a very reactive molecule with an unpaired electron).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies of the relationship between morphine and oxidative stress are not very abundant, and the results describing the pro-oxidant or antioxidant roles of the opioid are contradictory (4-13). …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line

Almeida

Costa-Malaquias

Nascimento³

et al. 2014

Braz J Med Biol Res

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Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2 but partially prevented lipid peroxidation caused by 0.0025% H2O2 (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2, opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line

Almeida

Costa-Malaquias

Nascimento³

et al. 2014

Braz J Med Biol Res

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“…31,32 These anti-inflammatory actions may be responsible for the antiallodynic effects of thioctic acid in neuropathy.…”

Section: Timepointmentioning

confidence: 99%

The effect of thioctic acid on allodynia in a rat vincristine-induced neuropathy model

et al. 2015

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Objective: To investigate the antiallodynic effects of thioctic acid in vincristine-induced neuropathy in rats. Methods: Neuropathy was induced in Sprague-Dawley rats via vincristine intraperitoneal injection. After 15 days, rats were investigated for the presence of mechanical and cold allodynia, and those with allodynia received intraperitoneal injection with normal saline or 1, 5, or 10 mg/kg thioctic acid. Mechanical and cold allodynia were assessed before treatment and at 15, 30, 60, 90, 150 and 180 min after treatment. Results: Mechanical and cold allodynia were reduced by thioctic acid injection. The duration of effect increased with thioctic acid dose. Conclusion: Thioctic acid may be an effective treatment for vincristine-induced neuropathy.

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Alpha‐lipoic acid: A promising adjuvant for nonsteroidal anti‐inflammatory drugs therapy with improved efficacy and gastroprotection

Barut

Engin

Sayğın

et al. 2021

Drug Development Research

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Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used in a wide variety of diseases due to their analgesic and anti-inflammatory effects, but their usage have been limited due to significant ulcerogenic side effects. In the present study, we aimed to evaluate the effect of α-lipoic acid (ALA) treatment on the anti-inflammatory activity of indomethacin (Indo) as well as the possible therapeutic effect of ALA on high dose Indo-induced gastropathy in female mice. Mice were treated with Indo (5 or 30 mg/kg, p.o) alone or in combination with ALA (50, 100 or 200 mg/kg, i.p). in vivo anti-inflammatory effect was evaluated by formalin-induced paw edema measured as paw thickness and edema. Gastric damage was evaluated macroscopically and histologically by scoring mucosal hemorrhage, erosion, edema and inflammation.

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Role of oxidative stress and inducible nitric oxide synthase in morphine-induced tolerance and dependence in mice. Effect of alpha-lipoic acid

Cited by 67 publications

References 40 publications

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line

The effect of thioctic acid on allodynia in a rat vincristine-induced neuropathy model

Alpha‐lipoic acid: A promising adjuvant for nonsteroidal anti‐inflammatory drugs therapy with improved efficacy and gastroprotection

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