Role of Oxidant/Antioxidant Status in the Pathogenesis of Iron-Deficiency Anemia: Case Study on Children of Qalyubiyya and Minoufiya Governorates, Egypt

Mehram, Eman B; Ahmed, Sara A. Sayed; Elhassaneen, Yousif

doi:10.21608/bnni.2021.164215

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Such effect could be attributed to the high levels content of different bioactive compounds including phytochemicals (phenolics, flavonoids, carotenoids, lycopene , tannins, anthocyanin's, polysaccharides and chlorophyll) and vitamins (A, C and E) found in such extract. Our previous and present data indicated that all of those compounds exert different highly biological activities including antioxidant, scavenging free radicals and inhibiting the lipid peroxidation [23,30,72] Due to their biological properties, WPEE can inhibit the cellular oxidative stress and is prevent the oxidative damage to DNA/RNA.…”

Section: Deoxyribonucleic Acid (Dna)mentioning

confidence: 99%

Untitled

2022

JBR

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Benzo[a]pyrene (B[a]P), a ubiquitous and prototypical environmental and dietary toxicant, promotes oxidative stress and DNA damage. Plant parts such as watermelon (Citrullis Lanatus) peel have potent antioxidative activity that protects cells from oxidative stress and cellular damage. In the present study, we evaluate the bioactive compounds content and antioxidant activity of watermelon peel ethanolic extract (WPEE) and its potential role in attenuating B[a]P exposureinduced molecular damage in liver cells in vitro. WPEE showed high amounts of bioactive compounds, which include polyphenols, polysaccharides, lycopene carotenoids, flavonoids, tannins, anthocyanins, chlorophyll and some vitamins such as A, C and E besides high antioxidant activity. Liver cells cultures subjected to 10 µM of B[a]P leads to many adverse effects including cytotoxic effect (inhibition the cell growth) and damage to macromolecules, such as DNA and RNA measured by Electrophoretic pattern. This induction was markedly reduced after WPEE Co-treatment by the concentrations 25, 50, 75 and 100 µg.ml -1 which exhibited a dose-dependent efficacy. In conclusion, B[a]P-induced cytotoxicity occurs through DNA damage, cell cycle arrest and ROS production. WPEE could reverse some of these B[a]P-mediated alterations and therefore be effective natural compounds against the adverse effects of B[a]P in liver cells.

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Section: Deoxyribonucleic Acid (Dna)mentioning

confidence: 99%

Untitled

2022

JBR

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“…It is thought that oxidative stress increases with the elevation in pro-oxidant levels or decrease in antioxidant enzyme capacities in IDA. Due to oxidative stress, the loss of balance between free radicals or ROS production and the antioxidant system leads to deterioration in molecular and cellular functions (7,8). Thiols, a group of organic sulfur compounds, occupy an important place in the antioxidant system and protect the organism against the harmful effects of ROS by coordinating the antioxidant defense (9).…”

Section: Introductionmentioning

confidence: 99%

Dynamic Thiol-Disulfide Homeostasis in Children with Newly Diagnosed Iron Deficiency Anemia

Kurucu

Fettah

Yeşil

et al. 2023

Türkiye Çocuk Hast Derg

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Objective: Iron is an element, which is found in the structure of antioxidant enzymes and has an important role in the inactivation of reactive oxygen species. Disruption of oxidant-antioxidant balance may be playing a role in the pathogenesis of iron deficiency anemia (IDA). Dynamic thiol-disulfide homeostasis (DTDH) and serum ischemia-modified albumin (IMA) levels are important indicators of pro-oxidant/antioxidant status. In this study, we aimed to evaluate DTDH parameters and serum IMA levels in children with newly diagnosed IDA, who did not receive iron therapy. Material and Methods: Fifty patients diagnosed with IDA and 33 healthy age- and sex-matched control patients were included in the study. DTDH parameters and IMA levels of the patients and control groups were measured. The same parameters were also compared in patients with Hb<7 g/dl (profound IDA) (n:14/50, 28%) and Hb≥7 g/dl (mild-moderate IDA) (n: 36/50, 72%) in the IDA group. The relationship between DTDH parameters in these groups were investigated. Results: Native thiol, total thiol, native thiol/total thiol levels, constituting antioxidant capacity indicators, were found to be significantly lower in IDA patients; while oxidant disulfide, disulfide/native thiol, disulfide/total thiol, and IMA levels were found to be statistically higher compared to those in the control group (p<0.050). When DTDH parameters and IMA levels were examined; there was a positive correlation between antioxidant parameters and a negative correlation between oxidative parameters with hemoglobin and ferritin levels (p<0.050). Also, oxidative parameters were found to be much higher in profound IDA group than in the group with Hb>7 g/dl (p<0.050). Conclusion: In this study, increase in serum disulfide and IMA levels with the decrease in serum native thiol and total thiol levels indicated oxidative stress in IDA patients before treatment, compared to the control group. Evaluation of these indicators in children is important in predicting the toxicity due to IDA.

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