Role of outer membrane protein T in pathogenicity of avian pathogenic Escherichia coli

Hejair, Hassan M.A.; Ma, Jiale; Zhu, Yan; Sun, Min; Wang, Dong; Zhang, Yue; Pan, Zihao; Zhang, Wei; Yao, Huochun

doi:10.1016/j.rvsc.2017.01.026

Cited by 21 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OmpT protein may have a role in adherence to eukaryotic cells [ 17 ], and may be implicated in NMEC virulence because it is highly regulated in human brain microvascular endothelial cells [ 18 ]. OmpT is important to the pathogenicity of APEC, and has a role in adherence and invasion of host cells [ 19 ]. Lastly, TraT is encoded by large plasmids, typically F-like conjugative plasmids and has functions in plasmid transfer [ 20 ] and serum resistance [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

A recombinant multi-antigen vaccine with broad protection potential against avian pathogenic Escherichia coli

Goor¹,

Stromberg²,

Mellata³

2017

PLoS ONE

View full text Add to dashboard Cite

Chickens are a major source of protein worldwide, yet infectious diseases continue to threaten the poultry industry. Avian pathogenic Escherichia coli (APEC), a subgroup of extraintestinal pathogenic E. coli (ExPEC), causes colibacillosis in chickens resulting in economic loss because of treatment, condemnation of products, and death. In this study, we evaluated a recombinant antigens (rAg) vaccine combining common ExPEC surface proteins EtsC, OmpA, OmpT, and TraT for broad protective potential against APEC infections in chickens. The specific objectives were to evaluate antibody (serum) and cytokines (lymphoid organs) responses to vaccination; in vitro bactericidal ability of serum and splenocytes against multiple APEC serotypes; and in vivo protection against APEC challenge in chickens. Groups of four-day old chickens (N = 10) were vaccinated twice (two-week interval) subcutaneously with rAgs alone or in combination and CpG adjuvant or PBS (control). IgY antibody in the serum and mRNA expression of IL-1β, IL-6, IL-18, IFN-γ, IL-4, IFN-β, and IL-8 in bursa, spleen, and thymus were measured using ELISA and RT-qPCR, respectively. Serum and splenocytes were tested for their bactericidal ability in vitro against multiple APEC isolates. Vaccinated and non-vaccinated chickens were challenged with 108 CFU of APEC-O2 via air sac at 31 days post first vaccination. Vaccine protection was determined by the decrease of bacterial loads in blood and organs (lung, heart, spleen, and liver), as well as gross colibacillosis lesion scores in air sac, heart, and liver. Vaccination significantly (P < 0.05) elicited IgY against specific antigens, induced immune related mRNA expression in the spleen and bursa, reduced in vitro growth of multiple APEC serotypes, and decreased bacterial loads in the heart and spleen, and gross lesion scores of the air sac, heart and liver in chickens. The vaccine reported may be used to provide broad protection against APEC strains, increasing animal welfare and food production.

show abstract

Section: Introductionmentioning

confidence: 99%

A recombinant multi-antigen vaccine with broad protection potential against avian pathogenic Escherichia coli

Goor¹,

Stromberg²,

Mellata³

2017

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Recent studies have shown that the OmpT protein contributes to the adhesion of the ExPEC and APEC strains [ 39 , 40 , 41 ]. Consequently, it was interesting to evaluate whether OmpT also contributes to the adhesion of STEC.…”

Section: Resultsmentioning

confidence: 99%

“…As mentioned, we also identified the OmpT protein as an antigen of STEC. The product of the ompT gene is a protease with a well-known role in the virulence of extraintestinal pathogenic E. coli (ExPEC), avian pathogenic E. coli (APEC) as well as in DEC strains [ 35 , 36 , 37 , 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

“…The proteolytic activity of OmpT mediates resistance against antimicrobial peptides such as protamine and cathelicidin LL-37 [ 35 , 36 , 37 ], and it is involved in the biogenesis of outer membrane vesicles (OMVs) [ 38 ]. In addition, some studies have suggested that OmpT participates in the adhesion of ExPEC and APEC strains [ 39 , 40 , 41 ]; however, whether this additional role depends on its proteolytic activity has not been demonstrated conclusively to date.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli

et al. 2020

View full text Add to dashboard Cite

Shiga toxin-producing Escherichia coli (STEC) causes outbreaks and sporadic cases of gastroenteritis. STEC O157:H7 is the most clinically relevant serotype in the world. The major virulence determinants of STEC O157:H7 are the Shiga toxins and the locus of enterocyte effacement. However, several accessory virulence factors, mainly outer membrane proteins (OMPs) that interact with the host cells may contribute to the virulence of this pathogen. Previously, the elongation factor thermo unstable (EF-Tu), l-asparaginase II and OmpT proteins were identified as antigens in OMP extracts of STEC. The known subcellular location of EF-Tu and l-asparaginase II are the cytoplasm and periplasm, respectively. Therefore, we investigate whether these two proteins may localize on the surface of STEC and, if so, what roles they have at this site. On the other hand, the OmpT protein, a well characterized protease, has been described as participating in the adhesion of extraintestinal pathogenic E. coli strains. Thus, we investigate whether OmpT has this role in STEC. Our results show that the EF-Tu and l-asparaginase II are secreted by O157:H7 and may also localize on the surface of this bacterium. EF-Tu was identified in outer membrane vesicles (OMVs), suggesting it as a possible export mechanism for this protein. Notably, we found that l-asparaginase II secreted by O157:H7 inhibits T-lymphocyte proliferation, but the role of EF-Tu at the surface of this bacterium remains to be elucidated. In the case of OmpT, we show its participation in the adhesion of O157:H7 to human epithelial cells. Thus, this study extends the knowledge of the pathogenic mechanisms of STEC.

show abstract

“…( 31 ) compared APEC and AFEC strains and revealed that a bigger set of genes might be needed to differentiate pathogenic from non-pathogenic bacteria well. Therefore, in this study, a set of 16 factors reported previously ( 7 , 12 , 14 , 26 , 28 ) as virulent for poultry were chosen and in silico analysis of 14 original APEC genomes with 16 strains regarded as APEC was performed and the results deposited in the NCBI database. Our results characterised the analysed strains by the varying content of selected virulence associated genes ranging from 2 to 15 VAGs.…”

Section: Discussionmentioning

confidence: 99%

In silico analysis of virulence associated genes in genomes of Escherichia coli strains causing colibacillosis in poultry

Kołsut¹,

Borówka

Marciniak

et al. 2017

Journal of Veterinary Research

View full text Add to dashboard Cite

IntroductionColibacillosis – the most common disease of poultry, is caused mainly by avian pathogenic Escherichia coli (APEC). However, thus far, no pattern to the molecular basis of the pathogenicity of these bacteria has been established beyond dispute. In this study, genomes of APEC were investigated to ascribe importance and explore the distribution of 16 genes recognised as their virulence factors.Material and MethodsA total of 14 pathogenic for poultry E. coli strains were isolated, and their DNA was sequenced, assembled de novo, and annotated. Amino acid sequences from these bacteria and an additional 16 freely available APEC amino acid sequences were analysed with the DIFFIND tool to define their virulence factors.ResultsThe DIFFIND tool enabled quick, reliable, and convenient assessment of the differences between compared amino acid sequences from bacterial genomes. The presence of 16 protein sequences indicated as pathogenicity factors in poultry resulted in the generation of a heatmap which categorises genomes in terms of the existence and similarity of the analysed protein sequences.ConclusionThe proposed method of detection of virulence factors using the capabilities of the DIFFIND tool may be useful in the analysis of similarities of E. coli and other sequences deriving from bacteria. Phylogenetic analysis resulted in reliable segregation of 30 APEC strains into five main clusters containing various virulence associated genes (VAGs).

show abstract

Role of outer membrane protein T in pathogenicity of avian pathogenic Escherichia coli

Cited by 21 publications

References 29 publications

A recombinant multi-antigen vaccine with broad protection potential against avian pathogenic Escherichia coli

A recombinant multi-antigen vaccine with broad protection potential against avian pathogenic Escherichia coli

Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli

In silico analysis of virulence associated genes in genomes of Escherichia coli strains causing colibacillosis in poultry

Contact Info

Product

Resources

About