Role of Oral Microbial Infections in Oral Cancer

Ferguson, Brett L.; Barber, Scott; Asher, Imani H.; Wood, Chalmers R.

doi:10.1016/j.cden.2016.12.009

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…Our results also depict the increase in abundance of genus Porphyromonas in OC and tobacco groups as compared to healthy individuals. Porphyromonas gingivalis is a well-studied and known periodontal pathogen [35,36]. P. gingivalis attaches to the epithelial cells of the oral cavity, causes inflammation, evades host defence mechanisms by utilizing numerous virulence factors, and causes deregulation of innate immunity and inflammatory responses [37].…”

Section: Discussionmentioning

confidence: 99%

Identification & correlation of bacterial diversity in oral cancer and long-term tobacco chewers- A case-control pilot study

Sawant

Dugad

Parikh

et al. 2021

Journal of Medical Microbiology

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Introduction. Squamous cell carcinoma is a highly aggressive type of oral cancer (OC). It is the most common cancer among men, and accounts for almost 90 % of all oral cancers in India. Consumption of tobacco is a leading factor contributing to maximum oral cancer incidences as per the WHO. Hypothesis/Gap statement. Researchers reported a direct association of microorganisms with dysbiosis in various oral lesions including oral cancer. However, there is a dearth of information related to compositional changes in the oral microbiome in long-term tobacco chewers and the Indian oral cancer population. Aim. The aim of this study was to identify and correlate the bacterial diversity in the oral cavity of tobacco chewers, patients with oral cancer and healthy subjects in the Indian population. Methods. Oral rinse samples were collected for ten subjects in each group followed by DNA extraction. The variable regions of the bacterial 16S rRNA gene (V6-V8) were amplified, sequenced, processed, and analysed using QIIME2 platform to assess alpha and beta diversity between the study groups. Results. This pilot study showed genus Streptococcus dominated the control group (18.54 %), and the abundance decreased in tobacco and OC group (9.63 and 5.45% respectively); whereas genus Prevotella dominated the tobacco and OC group (21.01 and 26.03% respectively). A shift in abundance of microbiome was observed from control population to oral cancer via the tobacco chewing population. Maximum alpha diversity of oral microbiome was found in Indian tobacco chewers. Beta diversity of tobacco chewers was similar to both the healthy population as well as oral cancer patients suggesting transitioning of the oral microbiome from healthy to oral cancer microbiome via the tobacco chewers microbiome. Conclusion. The data provides evidence of oral bacterial dysbiosis due to tobacco chewing habits that can further lead to progression towards cancer.

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Section: Discussionmentioning

confidence: 99%

Identification & correlation of bacterial diversity in oral cancer and long-term tobacco chewers- A case-control pilot study

Sawant

Dugad

Parikh

et al. 2021

Journal of Medical Microbiology

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“…CHIN-FU CHEN 1 , JAI-SING YANG 2 , WEN-KANG CHEN 3 , CHI-CHENG LU 4 , JO-HUA CHIANG 5 , HONG-YI CHIU 4,6,7 , SHIH-CHANG TSAI 8 , YU-NING JUAN 2 , HAO-JEN HUANG 1,9 and TZONG-DER WAY 8 according to reactive epithelial or pre-neoplasia preneoplastic changes (9,10). The current chemotherapeutic agents for OSCC are cisplatin, 5-fluorouracil, bleomycin, mitomycin-c, methotrexate, oxaliplatin and tegafur/uracil (11,12).…”

Section: Ursolic Acid Elicits Intrinsic Apoptotic Machinery By Downrementioning

confidence: 99%

“…Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck cancer in the world and accounts for ~95% of all cases of head and neck cancer (1,2). Patients with OSCC have a five-year survival rate of ~50%, with the proportion of men with oral squamous cell carcinoma being higher than that of women (2,3). The symptoms of OSCC include erythroplakia, leukoplakia, ulcers in the mouth, necrosis of the surface tissue, formation of an intermediate cavity or an uneven surface mass, bleeding, and swelling of the submandibular and cervical lymph nodes (4,5).…”

Section: Introductionmentioning

confidence: 99%

Ursolic acid elicits intrinsic apoptotic machinery by downregulating the phosphorylation of AKT/BAD signaling in human cisplatin‑resistant oral cancer CAR cells

et al. 2018

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Oral squamous cell carcinoma (OSCC) is a type of cancer with high morbidity and mortality rates worldwide; it also demonstrates chemotherapeutic resistance. Triterpenoid ursolic acid has been shown to exhibit various biological activities and anticancer effects in several preclinical studies. In our previous study, human cisplatin‑resistant oral cancer CAR cells were established, and the present study aimed to further examine the effects of ursolic acid on CAR cells. The results revealed that ursolic acid inhibited CAR cell viability, as determined using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. Ursolic acid‑induced cell death was mediated through a caspase‑dependent pathway, determined with the pan‑caspase inhibitor, z‑VAD‑fmk. Ursolic acid also increased the activities of caspase‑3 and caspase‑9 in CAR cells, determined by a colorimetric assay. Specifically, the production of reactive oxygen species and loss of mitochondrial membrane potential, detected by flow cytometry, were observed in the ursolic acid‑treated CAR cells. The apoptosis‑associated signaling showed that ursolic acid decreased the phosphorylation of AKT (Ser473) and B‑cell lymphoma 2 (Bcl‑2)‑associated agonist of cell death (BAD; Ser136), and the protein levels of Bcl‑2 and Bcl‑extra large (Bcl‑xL), and increased the expression of BAD and Bcl‑2‑associated X (Bax) protein in CAR cells. In summary, the results supported the potential application of ursolic acid against drug‑resistant oral carcinoma and to improve oral anticancer efficacy in the near future.

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“…Although incipient and discreet, the interest in the bacterial performance in the oral carcinogenesis has increased. Some studies reported the considerable proliferation of aerobic and anaerobic micro-organisms in the biofilm present in oral carcinoma in humans 7 . This study observed that the bacterial contamination in the explants were relatively controlled with success, however, when there is an exacerbated bacterial proliferation, the keratinocytes expansion stops.…”

Section: /7mentioning

confidence: 99%

Primary cell culture of head and neck cancer: a challenge

Santos¹,

Goto²,

Pereira³

et al. 2018

Arch. Head Neck Surg.

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Introduction: Head and neck cancer presents a high rate of recurrence and mortality, considering the sites affected. The use of primary culture allows pre-clinical trials that would not be possible in humans or would require a long time until the initial tests were approved. Objective: To establish primary culture of carcinomas and the disease-free surgical margin of individuals affected by neck cancer. Methods: Fragments of 6 cases of oral cavity carcinoma and 2 cases of non-malignant tissue (surgical margin) of patients with oral cancer were collected immediately after surgical resection. These specimens were packed in complete DMEM (Dulbecco Modified Eagle's Medium, SIGMA  ) supplemented with 10% inactivated Bovine Fetal Serum (BFSi) and 5% antibiotic / antimycotic -and kept on ice for transportation to the Molecular Marker Laboratory And Cancer Cell Signaling in FCFRP-USP. Processing was carried out in a biosafety booth in a cell culture room, 2 hours after collection maximum. All specimens collected were advanced tumors of the oral cavity. Results: From the 6 cases collected and kept in culture, only 2 presented uncontrollable bacterial contamination and were discarded. Two other cases released fibroblasts in the first 3 to 5 days and the observation of neoplastic cells (keratinocytes) was only possible after seven to ten days. Both cell types exhibited monolayer expansion. Conclusion: The use of explants to establish the initial stages of primary culture of head and neck cancer is a viable and easily reproducible alternative. The effective success rate is achieved in 20-30% of the cases and the control of the contamination presents itself as one of the biggest obstacles to be surpassed in the initial stages of cultivation.

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Role of Oral Microbial Infections in Oral Cancer

Cited by 11 publications

References 22 publications

Identification & correlation of bacterial diversity in oral cancer and long-term tobacco chewers- A case-control pilot study

Identification & correlation of bacterial diversity in oral cancer and long-term tobacco chewers- A case-control pilot study

Ursolic acid elicits intrinsic apoptotic machinery by downregulating the phosphorylation of AKT/BAD signaling in human cisplatin‑resistant oral cancer CAR cells

Primary cell culture of head and neck cancer: a challenge

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