2009
DOI: 10.1111/j.1365-3059.2009.02141.x
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Role of oospores as primary inoculum for epidemics of downy mildew caused by Peronospora arborescens in opium poppy crops in Spain

Abstract: This study investigated the role of Peronospora arborescens oospores as primary inoculum for downy mildew of opium poppy and infection types that they may give rise to in Spain using an integrative experimental approach that combined pathogenicity tests in growth chambers and field microplots, together with molecular detection of P. arborescens infection by specific nested-PCR assays. The results demonstrated that oospores in infested soil or leaf debris were effective inoculum for ingress of the pathogen thro… Show more

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Cited by 22 publications
(20 citation statements)
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“…More interestingly, use of the nested-PCR assay allowed detection of P. somniferi in 20 to 100% seed samples from symptomless capsules, as well as from capsules of all the genotypes rated as resistant due to the absence of DM symptoms. Comparatively, use of primers OMPac7fw/OMPac7rv in single PCR assays allowed detection of the pathogen in 50 to 75% of seed samples in only three of the nine resistant genotypes evaluated (Table 1) Therefore, results show that the 'drop' inoculation of opium poppy leaves with a suspension of P. somniferi sporangia is efficient for establishing infection and reproducing the chlorotic and deformation syndrome frequently observed in DM affected-plants in commercial fields (Montes-Borrego et al, 2009a). This 'drop' inoculation method would be particularly useful when assessing a large number of opium poppy varieties under field conditions, since the amount of inoculum needed is 40X times lower than that for the spray inoculation method used previously (data not shown).…”
Section: Resultsmentioning
confidence: 94%
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“…More interestingly, use of the nested-PCR assay allowed detection of P. somniferi in 20 to 100% seed samples from symptomless capsules, as well as from capsules of all the genotypes rated as resistant due to the absence of DM symptoms. Comparatively, use of primers OMPac7fw/OMPac7rv in single PCR assays allowed detection of the pathogen in 50 to 75% of seed samples in only three of the nine resistant genotypes evaluated (Table 1) Therefore, results show that the 'drop' inoculation of opium poppy leaves with a suspension of P. somniferi sporangia is efficient for establishing infection and reproducing the chlorotic and deformation syndrome frequently observed in DM affected-plants in commercial fields (Montes-Borrego et al, 2009a). This 'drop' inoculation method would be particularly useful when assessing a large number of opium poppy varieties under field conditions, since the amount of inoculum needed is 40X times lower than that for the spray inoculation method used previously (data not shown).…”
Section: Resultsmentioning
confidence: 94%
“…Seeds were proven free of infection by P. somniferi by molecular testing using a specific-PCR assay (Montes-Borrego et al, 2009a). Out of the 20 genotypes, nine (designated R1-R9) and five (designated S1-S5) genotypes had previously been characterized as potentially resistant and susceptible to P. somniferi, respectively, in field experiments under natural inoculum pressure (Montes-Borrego et al, unpublished results), three genotypes (designated M8, EA and TAR) are commercial varieties, and the remaining three genotypes (designated F1-1, F1-2 and F1-3) are F1 progenies from crosses between TAR and R9 genotypes.…”
Section: Plant Materialsmentioning
confidence: 99%
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