Role of Nuclear Factor-κB in Oxidative Stress Associated with Rabies Virus Infection of Adult Rat Dorsal Root Ganglion Neurons

Kammouni, Wafa; Hasan, Leena; Saleh, Anas; Wood, Heidi; Fernyhough, Paul; Jackson, Alan C.

doi:10.1128/jvi.00550-12

Cited by 18 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, ciliary neurotropic factor, a neurotrophic factor and NF-κB activator, significantly reduced the number of 4-HNE puncta in the infected neurons (Kammouni et al, 2012). Quantitative assessment of NF-kB p50 subunit levels in nuclear and cytoplasm indicated higher nuclear to cytoplasm ratio in CVS-infected cells versus mock-infected neurons at 24 hours post infection, but the nuclear localization of p50 subunit was impaired at 48 and 72 hours post-infection (Kammouni et al, 2012). It was concluded that rabies virus induces oxidative stress in the infected neurons by inhibiting NF-kB activation (Kammouni et al, 2012) although other cellular mechanisms may also be involved.…”

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 95%

“…CVS infection in the presence of SN50, a peptide that prevents NF-κB translocation to the nucleus, increased the 4-HNE puncta in the axons of infected neurons (Kammouni et al, 2012). On the other hand, ciliary neurotropic factor, a neurotrophic factor and NF-κB activator, significantly reduced the number of 4-HNE puncta in the infected neurons (Kammouni et al, 2012). Quantitative assessment of NF-kB p50 subunit levels in nuclear and cytoplasm indicated higher nuclear to cytoplasm ratio in CVS-infected cells versus mock-infected neurons at 24 hours post infection, but the nuclear localization of p50 subunit was impaired at 48 and 72 hours post-infection (Kammouni et al, 2012).…”

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 97%

“…Recently, evidence of oxidative stress generation, axonal swelling induction, and axonal outgrowth reduction was also observed in rabies virus-infected rat dorsal root ganglion cultured neurons (Kammouni et al, 2012). Nuclear factor kappa B (NF-κB) p50 and p65 subunit were overexpressed in CVS-infected neurons as indicated by western blotting analysis (Kammouni et al, 2012).…”

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 99%

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 99%

“…Nuclear factor kappa B (NF-κB) p50 and p65 subunit were overexpressed in CVS-infected neurons as indicated by western blotting analysis (Kammouni et al, 2012). CVS infection in the presence of SN50, a peptide that prevents NF-κB translocation to the nucleus, increased the 4-HNE puncta in the axons of infected neurons (Kammouni et al, 2012). On the other hand, ciliary neurotropic factor, a neurotrophic factor and NF-κB activator, significantly reduced the number of 4-HNE puncta in the infected neurons (Kammouni et al, 2012).…”

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 99%

See 4 more Smart Citations

Mitochondrial dysfunction in rabies virus infection of neurons

et al. 2013

Self Cite

View full text Add to dashboard Cite

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 95%

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 97%

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 99%

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 99%

Section: Rabies Virus-induced Oxidative Stressmentioning

confidence: 99%

See 3 more Smart Citations

Mitochondrial dysfunction in rabies virus infection of neurons

et al. 2013

Self Cite

View full text Add to dashboard Cite

Serine residues at positions 162 and 166 of the rabies virus phosphoprotein are critical for the induction of oxidative stress in rabies virus infection

2016

View full text Add to dashboard Cite

Our previous work in a mouse model of experimental rabies showed neuronal process (dendrites and axons) degeneration in association with severe clinical disease. Cultured adult rodent dorsal root ganglion (DRG) neurons infected with the challenge virus standard-11 (CVS) strain of rabies virus (RABV) showed axonal swellings and reduced axonal growth with evidence of oxidative stress. We have shown that CVS infection alters a variety of mitochondrial parameters and increases mitochondrial complex I activity and reactive oxygen species (ROS) production. Expression of a peptide from amino acid 139-172 of the CVS phosphoprotein (P) increased complex I activity and ROS generation similar to expression of the entire P. Site-directed mutational analyses illustrated the importance of the 145-151 and 157-169 regions of P and that serine residues at 162 and 166 are important single amino acid sites. Two CVS recombinant viruses with serine to alanine mutations at positions 162 (A162r) and 166 (A166r) did not increase complex I activity or ROS generation and also did not induce axonal swellings or inhibit axonal growth in DRG neurons. RABV infection is a mitochondrial disorder initiated by interaction of the RABV P and complex I; S162 and S166 are critical sites in the P for this interaction. The resulting mitochondrial dysfunction produces oxidative stress in neurons causing acute degenerative changes affecting neuronal processes resulting in a severe and fatal clinical disease. This information will be important for the future development of novel therapies for rabies.

show abstract

Lyssavirus phosphoproteins increase mitochondrial complex I activity and levels of reactive oxygen species

2017

View full text Add to dashboard Cite

We have previously demonstrated that serine residues at positions 162 and 166 of the rabies virus (RABV) phosphoprotein (P) are critical for oxidative stress induced by CVS in cultured cells. We have now evaluated the P of two street RABV variants and Mokola (MOK) virus. The P of these viruses, like CVS, induces an increase in complex I activities and reactive oxygen species levels in transfected cells. Although the sequence homology of P is only 45% with MOK (higher for street viruses) and CVS, serine residues are conserved at positions 162 and 166, suggesting their potential importance in oxidative stress.

show abstract

Role of Nuclear Factor-κB in Oxidative Stress Associated with Rabies Virus Infection of Adult Rat Dorsal Root Ganglion Neurons

Cited by 18 publications

References 37 publications

Mitochondrial dysfunction in rabies virus infection of neurons

Mitochondrial dysfunction in rabies virus infection of neurons

Serine residues at positions 162 and 166 of the rabies virus phosphoprotein are critical for the induction of oxidative stress in rabies virus infection

Lyssavirus phosphoproteins increase mitochondrial complex I activity and levels of reactive oxygen species

Contact Info

Product

Resources

About