1991
DOI: 10.1093/infdis/163.6.1256
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Role of Normal Serum in the Binding of Lipopolysaccharide to IgG Fractions from Rabbit Antisera to Escherichia coli J5 and Other Gram-Negative Bacteria

Abstract: Because lipopolysaccharide (LPS) bound to lipoprotein is less active than unbound LPS in multiple assay systems, the binding of radiolabeled LPS to lipoproteins in sera prepared from normal rabbits and rabbits made hyperimmune to Escherichia coli J5 were compared. LPS-lipoprotein binding in hyperimmune sera to E. coli J5 was not greater than that in normal serum as assessed by ultracentrifugation, but more LPS was precipitated from hyperimmune antisera than normal sera under conditions designed to precipitate … Show more

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Cited by 10 publications
(9 citation statements)
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“…IgG was purified from hyperimmune serum, from artificial capillary cell culture supernatants, and from ammonium sulfate-precipitated ascites fluid by passage over a protein G-Sepharose 4 fast-flow column (Pharmacia, Piscataway, N.J.) as described elsewhere (27,64). Bound IgG was eluted from the column with 0.1 M glycine (pH 2.7) and was immediately neutralized with 1 M Tris buffer (pH 9.0).…”
mentioning
confidence: 99%
“…IgG was purified from hyperimmune serum, from artificial capillary cell culture supernatants, and from ammonium sulfate-precipitated ascites fluid by passage over a protein G-Sepharose 4 fast-flow column (Pharmacia, Piscataway, N.J.) as described elsewhere (27,64). Bound IgG was eluted from the column with 0.1 M glycine (pH 2.7) and was immediately neutralized with 1 M Tris buffer (pH 9.0).…”
mentioning
confidence: 99%
“…IgG was purified from ascites after ammonium sulfate precipitation [12] and from hyperimmune serum [14,15]. In brief, affinity chromatography was performed by passage over a protein G-sepharose 4 fast-flow column (Pharmacia, Piscataway, NJ).…”
mentioning
confidence: 99%
“…The plates were again washed three times, and peroxidase substrate (1 mg/mL H 2 O 2 Preparation of IgG fractions. IgG was prepared from hyperimmune serum as described [18,19]. Briefly, affinity chromatography in ABTS, citric acid, Na 2 HPO 4 ) was added.…”
mentioning
confidence: 99%