Role of nitro-oxidative stress in the pathogenesis of experimental rat periodontitis

Boşca, Bianca Adina; Mitre, Viorel; Ilea, Aranka; Câmpian, Radu Septimiu; Rus, Vasile; Ruxanda, Flavia; Rațiu, Cristian; Uifălean, Ana; Pârvu, Alina Elena

doi:10.15386/cjmed-529

Cited by 7 publications

(8 citation statements)

References 50 publications

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“…For morphometric analysis, the images were digitized using the Leica Biosistems ® Digital Image Hub online software (Wetzlar, Germany). The study variables were determined: thickness and structure of the epithelium, state of the basal membrane, quantitative measurement of the inflammatory area and the condensation of the connective tissue, according to the criteria described by authors such as Camacho-Alonso et al [23], Bosca et al [24] and Ionel et al [25].…”

Section: Pathological Anatomymentioning

confidence: 99%

Chemoprevention of Experimental Periodontitis in Diabetic Rats with Silk Fibroin Nanoparticles Loaded with Resveratrol

et al. 2020

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Objective: the objective of the present work is to study the effectiveness of treatment with silk fibroin nanoparticles loaded with resveratrol in experimental periodontitis in a diabetic rat model. Introduction: Periodontitis is an inflammatory pathology highly related to other diseases, such as type II diabetes. Both diseases have a specific inflammatory condition, with Interleukin (IL)-6, IL-1β and Transforming Grow Factor (TGF)-1β being the most relevant proinflammatory factors. Silk fibroin (SF) nanoparticles loaded with resveratrol (Res-SFN) are a new alternative as a treatment. Methods: 40 diabetic Sprague Dawley male rats were used and periodontitis was induced by ligation. The animals were divided into 5 treatment groups, and 1 mL of treatment was administered once a day for 4 weeks. The groups were: I: Carboxymethyl cellulose (CMC) 0.8%, II: CMC 0.8% + SF 1%, III: CMC 0.8% + RES-SFN 3 mg/mL, IV: CMC 0.8% + SF 1% + RES-SFN 3 mg/mL, V: Water. A peripheral blood sample was taken every week to quantify the inflammatory profile by ELISA (IL-6, IL-1β and TGF-1β). After 4 weeks the sacrifice was carried out and biopsies of the gum were taken. Results: Treatment with SF and RES-SFN reduced the amount of chemical inflammation mediators (with the exception of IL-1β in comparisons I-IV and II-IV (p > 0.05)), as well as the anatomopathological variables linked to it, in a significant way (p < 0.05). Conclusion: treatment with RES-SFN has reduced local inflammation in this experimental periodontitis model.

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Section: Pathological Anatomymentioning

confidence: 99%

Chemoprevention of Experimental Periodontitis in Diabetic Rats with Silk Fibroin Nanoparticles Loaded with Resveratrol

et al. 2020

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“…In such a rat model, it was shown that periodontitis leads to an increased production of reactive oxygen species and markers of oxidative damage (Ekuni et al, 2010 ). In addition, oxidative stress induced by periodontitis seems to be associated with the dynamics and severity of the periodontal inflammation (Bosca et al, 2016 ).…”

Section: Animal Experimentsmentioning

confidence: 99%

Oxidative Stress and Antioxidants in the Diagnosis and Therapy of Periodontitis

2017

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Oxidative stress has been implicated in the pathogenesis of numerous diseases. However, large interventional studies with antioxidants failed to show benefits in the prevention or treatment of cardiovascular diseases, cancer, or diabetes mellitus. Numerous clinical studies have confirmed the association of oxidative stress markers and periodontitis. Technical and biological variability is high for most of the analyzed markers and none of them seems to be optimal for routine clinical use. In a research setting, analysis of a palette of oxidative stress markers is needed to cover lipid peroxidation, protein oxidation, and the antioxidant status. The source of reactive oxygen species and their role in the pathogenesis of periodontitis remains unclear. Interventional experiments indicate that oxidative stress might be more than just a simple consequence of the inflammation. Small studies have confirmed that some antioxidants could have therapeutic value at least as an addition to the standard non-surgical treatment of periodontitis. A clear evidence for the efficiency of antioxidant treatment in large patient cohorts is lacking. Potentially, because lowering of oxidative stress markers might be a secondary effect of anti-inflammatory or antibacterial agents. As the field of research of oxidative stress in periodontitis gains attraction and the number of relevant published papers is increasing a systematic overview of the conducted observational and interventional studies is needed. This review summarizes the currently available literature linking oxidative stress and periodontitis and points toward the potential of adjuvant antioxidant treatment, especially in cases where standard treatment fails to improve the periodontal status.

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“…The results also revealed that the rats fed a high-fat diet and HW, compared with the HFD rats showed a lower distance between the CEJ-ABC and a greater BT/TV. Gingival oxidative stress is involved in the progression of alveolar bone resorption [ 11 , 12 ]. Suppression of obesity by drinking HW may attenuate alveolar bone resorption by limiting gingival oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro studies have shown that oxidative stress stimulates osteoclast differentiation [ 7 , 8 ]. Animal studies have also suggested that oxidative stress is involved in the progression of alveolar bone resorption [ 9 , 10 , 11 , 12 ]. In a recent review, the basis for the relationship between obesity and periodontitis lies at a fundamental intracellular level, which includes oxidative stress [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet

et al. 2017

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Obesity induces gingival oxidative stress, which is involved in the progression of alveolar bone resorption. The antioxidant effect of hydrogen-rich water may attenuate gingival oxidative stress and prevent alveolar bone resorption in cases of obesity. We examined whether hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption in obese rats fed a high-fat diet. Male Fischer 344 rats (n = 18) were divided into three groups of six rats each: a control group (fed a regular diet and drinking distilled water) and two experimental groups (fed a high-fat diet and drinking distilled water or hydrogen-rich water). The level of 8-hydroxydeoxyguanosine was determined to evaluate oxidative stress. The bone mineral density of the alveolar bone was analyzed by micro-computerized tomography. Obese rats, induced by a high-fat diet, showed a higher gingival level of 8-hydroxydeoxyguanosine and a lower level of alveolar bone density compared to the control group. Drinking hydrogen-rich water suppressed body weight gain, lowered gingival level of 8-hydroxydeoxyguanosine, and reduced alveolar bone resorption in rats on a high-fat diet. The results indicate that hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption by limiting obesity.

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Role of nitro-oxidative stress in the pathogenesis of experimental rat periodontitis

Cited by 7 publications

References 50 publications

Chemoprevention of Experimental Periodontitis in Diabetic Rats with Silk Fibroin Nanoparticles Loaded with Resveratrol

Chemoprevention of Experimental Periodontitis in Diabetic Rats with Silk Fibroin Nanoparticles Loaded with Resveratrol

Oxidative Stress and Antioxidants in the Diagnosis and Therapy of Periodontitis

Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet

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