Role of nitric oxide in the ethylcholine aziridinium model of delayed apoptotic neurodegeneration in vivo and in vitro

Phosphatidylinositol 3-Akt-Kinase-Dependent Phosphorylation of p21Waf1/Cip1as a Novel Mechanism of Neuroprotection by Glucocorticoids

“…The caspase dependence has been proven both by the broad-spectrum caspase inhibitor Z-VAD-FMK and the caspase-3-specific inhibitor Z-DQMD-FMK . The apoptotic cell death has been confirmed previously by DNA laddering, ␣-fodrin cleavage, terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling, Hoechst 33258 staining, and morphological appearance in phase-contrast microscopy Lautenschlager et al, 2000). Release of LDH into the culture medium served as an indirect marker of cell death.…”

Pneumococcal pneumolysin and H2O2 mediate brain cell apoptosis during meningitis

“…Colton, Georgetown University, Washington, DC, USA) that expresses most markers common to primary human microglial cells was cultured as previously described (9,21). Primary rat hippocampal neurons were isolated from fetal rats at embryonic day 18 as described (22). Briefly, hippocampus was dissected, incubated in trypsin-EDTA (Biochrom, Berlin, Germany), dissociated with a Pasteur pipette, and plated in starter medium (neurobasal medium and supplement B27; Invitrogen, Karlsruhe, Germany).…”

Neurotoxicity of Ecstasy Metabolites in Rat Cortical Neurons, and Influence of Hyperthermia

“…Primary neuronal cultures of cerebral cortex were obtained from embryos (E-18) of Wistar rats. Cultures were prepared according to Lautenschlager et al (2000). The cerebral cortex was dissected, meninges were removed, and tissue was incubated for 15 min in trypsin/EDTA [0.05:0.02% (w/v) in PBS] at 37°C; the cultures were rinsed twice with PBS and once with dissociation medium (modified Eagle's medium with 10% fetal calf serum, 10 mM HEPES, 44 mM glucose, 100 U penicillin plus streptomycin/ml, 2 mM Lglutamine, and 100 insulin units/l), dissociated by Pasteur pipette in dissociation medium, pelleted by centrifugation (210g for 2 min), redissociated in starter medium (neurobasal medium with supplemental B27, 100 U penicillin ϩ streptomycin/ml, 0.5 mM L-glutamine, and 25 M glutamate), and plated in 48-well plates in a density of 1.5 ϫ 10 5 cells/well.…”