Role of Nitric Oxide in Hydroxylamine Oxidation by Ammonia-Oxidizing Bacteria

Choi, Eunkyung; Chaudhry, Sana; Martens‐Habbena, Willm

doi:10.1128/aem.02173-22

Cited by 3 publications

(2 citation statements)

References 56 publications

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“…It has been reported that low concentrations of PTIO have a strong inhibitory effect on AOA rather than AOB ( Sun et al, 2022 ). For example, under the condition of 100 μM PTIO, PTIO can inhibit the ammonia oxidation process by inhibiting AOA ( Duan et al, 2018 ; Choi et al, 2023 ), and thus it was used to selectively inhibit the activity of AOA in the presence of AOB ( Kits et al, 2019 ). 3,4-dimethylpyrazole phosphate (DMPP) is a highly soluble compound with high stability and persistent suppression effects ( Li et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

Jia,

Zhou,

et al. 2024

Front. Microbiol.

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IntroductionAmmonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) have been proven to be key microorganisms driving the ammonia oxidation process. However, under different fertilization practices, there is a lack of research on the impact of interaction between predators and AOA or AOB on nitrogen cycling at the multi-trophic level.MethodsIn this study, a network-oriented microscopic culture experiment was established based on four different long-term fertilization practices soils. We used the nitrification inhibitors 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxide-3-oxyl (PTIO) and 3, 4-Dimethylpyrazole phosphate (DMPP) inhibited AOA and AOB, respectively, to explore the impact of interaction between protists and AOA or AOB on nitrogen transformation.ResultsThe results showed that long-term nitrogen application promoted the potential nitrification rate (PNR) and nitrous oxide (N2O) emission, and significantly increased the gene abundance of AOB, but had no obvious effect on AOA gene abundance. DMPP significantly reduced N2O emission and PNR, while PTIO had no obvious effect on them. Accordingly, in the multi-trophic microbial network, Cercozoa and Proteobacteria were identified as keystone taxa of protists and AOB, respectively, and were significantly positively correlated with N2O, PNR and nitrate nitrogen. However, Nitrososphaerota archaeon as the keystone species of AOA, had an obvious negative linkage to these indicators. The structural equation model (SEM) showed that AOA and AOB may be competitors to each other. Protists may promote AOB diversity through direct trophic interaction with AOA.ConclusionThe interaction pattern between protists and ammonia-oxidizing microorganisms significantly affects potential nitrification rate and N2O emission, which has important implications for soil nitrogen cycle.

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Section: Introductionmentioning

confidence: 99%

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

Jia,

Zhou,

et al. 2024

Front. Microbiol.

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“…While nitrate addition might stimulate both types of methanotrophs in paddy soil, ammonium addition only enriched type I methanotrophs while suppressing the growth of type II methanotrophs (Hu and Lu, 2015). While the methanotrophic strains lacking hydroxylamine oxidoreductase (HAO) are poor nitrifiers, the HAO-expressing strains may effectively oxidize ammonia (Nyerges and Stein, 2009;Stein and Klotz, 2011;Choi et al, 2023). Methane monooxygenases or ammonia monooxygenases linked to membranes oxidize ammonium in strains carrying the HAO gene, producing the intermediate hydroxylamine (Mohammadi et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional and metabolomic responses of Methylococcus capsulatus Bath to nitrogen source and temperature downshift

Bedekar,

Deewan,

Jagtap

et al. 2023

Front. Microbiol.

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Methanotrophs play a significant role in methane oxidation, because they are the only biological methane sink present in nature. The methane monooxygenase enzyme oxidizes methane or ammonia into methanol or hydroxylamine, respectively. While much is known about central carbon metabolism in methanotrophs, far less is known about nitrogen metabolism. In this study, we investigated how Methylococcus capsulatus Bath, a methane-oxidizing bacterium, responds to nitrogen source and temperature. Batch culture experiments were conducted using nitrate or ammonium as nitrogen sources at both 37°C and 42°C. While growth rates with nitrate and ammonium were comparable at 42°C, a significant growth advantage was observed with ammonium at 37°C. Utilization of nitrate was higher at 42°C than at 37°C, especially in the first 24 h. Use of ammonium remained constant between 42°C and 37°C; however, nitrite buildup and conversion to ammonia were found to be temperature-dependent processes. We performed RNA-seq to understand the underlying molecular mechanisms, and the results revealed complex transcriptional changes in response to varying conditions. Different gene expression patterns connected to respiration, nitrate and ammonia metabolism, methane oxidation, and amino acid biosynthesis were identified using gene ontology analysis. Notably, key pathways with variable expression profiles included oxidative phosphorylation and methane and methanol oxidation. Additionally, there were transcription levels that varied for genes related to nitrogen metabolism, particularly for ammonia oxidation, nitrate reduction, and transporters. Quantitative PCR was used to validate these transcriptional changes. Analyses of intracellular metabolites revealed changes in fatty acids, amino acids, central carbon intermediates, and nitrogen bases in response to various nitrogen sources and temperatures. Overall, our results offer improved understanding of the intricate interactions between nitrogen availability, temperature, and gene expression in M. capsulatus Bath. This study enhances our understanding of microbial adaptation strategies, offering potential applications in biotechnological and environmental contexts.

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Magnetite addition reduces nitrite requirement for efficient anaerobic ammonium oxidation by facilitating mutualism of ANAMMOX and FEAMMOX bacteria

Kadam,

Kim,

Yang

et al. 2024

Science of The Total Environment

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Role of Nitric Oxide in Hydroxylamine Oxidation by Ammonia-Oxidizing Bacteria

Cited by 3 publications

References 56 publications

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

Transcriptional and metabolomic responses of Methylococcus capsulatus Bath to nitrogen source and temperature downshift

Magnetite addition reduces nitrite requirement for efficient anaerobic ammonium oxidation by facilitating mutualism of ANAMMOX and FEAMMOX bacteria

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