Role of nitric oxide in inflammation and tissue injury during endotoxemia and hemorrhagic shock.

Shah, Nirav; Billiar, Timothy R.

doi:10.1289/ehp.98106s51139

Cited by 23 publications

(17 citation statements)

References 44 publications

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“…Further increase in NO levels on arginine supplementation confirmed the role of NO in liver injury as L-arginine is the precursor of NO synthesis (via the reaction catalyzed by nitric oxide synthase converting L-arginine to NO and citrulline). This is in agreement with the earlier studies that inducible form of nitric oxide synthase (iNOS) leads to the formation of excessive NO that causes tissue injury (Shah and Billiar, 1998). Further, the increased level of NO in the endotoxin-challenged rats may be associated with increased level of TNF-α observed in the present study.…”

Section: Discussionsupporting

confidence: 94%

Effect of Lactobacillus plantarum and l-arginine against endotoxin-induced liver injury in a rat model

et al. 2011

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Section: Discussionsupporting

confidence: 94%

Effect of Lactobacillus plantarum and l-arginine against endotoxin-induced liver injury in a rat model

et al. 2011

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“…Thus, NO, depending on its concentration, the biological redox milieu, and the involvement and/or induction of protective regimes can affect complex biological stimulatory as well as inhibitory gene responses. NO and ROS react each other forming toxic products which influence on motility and viability of cells by increasing expression and activation of various MMPs, including MMP-2/9 [41][42][43]. Within the past few years, many researchers have demonstrated the Effect of SNAP on VEGF-A mRNA was further confirmed using real time PCR.…”

Section: Discussionmentioning

confidence: 99%

Insidious role of nitric oxide in migration/invasion of colon cancer cells by upregulating MMP-2/9 via activation of cGMP-PKG-ERK signaling pathways

Babykutty

Suboj

Srinivas

et al. 2012

Clin Exp Metastasis

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Nitric oxide (NO), an uncharged free radical is implicated in various physiological and pathological processes. The present study is an investigation on the effect of NO on proliferation, apoptosis and migration of colon cancer cells. Colon adenocarcinoma cells, WiDr, were used for the in vitro experiments. Tissues from colon adenocarcinoma, adjacent normal and inflammatory tissue and lymph node with metastasis were evaluated for iNOS, MMP-2/9 and Fra-1/Fra-2. NO increases the proliferation of cancer cells and simultaneously prevents apoptosis. Expression of MMP-2/9, RhoB and Rac-1 was enhanced by NO in a time dependent manner. Further, NO increased phosphorylation of ERK1/2 and induced nuclear translocation of Fra-1 and Fra-2. Electrophoretic mobility shift analysis and use of deletion mutant promoter constructs identified role of AP-1 in NO-mediated regulation of MMP-2/9. iNOS, MMP-2/9, Fra-1 and Fra-2 in normal and colon adenocarcinoma tissues were analyzed and it was found that increased expression of these proteins in cancer when compared to normal provides support to our in vitro findings. The study showed that the NO-cGMP-PKG promotes MMP-2/9 expression by activating ERK-1/2 and AP-1. This study reveals the insidious role of NO in imparting tumor aggressiveness.

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“…Under pathologic circumstances, NO can be toxic to cells, inducing severe cellular damage or even cellular death. 89 Sepsis is associated with characteristic hemodynamic changes, including a profound vasodilation leading to hypotension. 90,91 This is classically measured as systemic vascular resistance index (SVRI) a number that is inversely proportional to the degree of vasodilation.…”

Section: Arginine In Myeloid Cellsmentioning

confidence: 99%

A Rational Use of Immune Enhancing Diets: When Should We Use Dietary Arginine Supplementation?

2004

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Controversies in any arena of human activity often result in the polarization of the individuals involved into 2 opposing camps. Controversy about the use of immune-enhancing diets (IEDs) is no exception. On one hand, some groups are proposing indiscriminate use of IEDs, whereas others have created guidelines advocating that their use should be banned for the critically ill. At stake is an emerging paradigm: that dietary manipulation of the immune system is possible and may become an important adjunct to other therapies, thus helping prevent or treat multiple diseases for millions worldwide. Under these circumstances, extremist claims of miraculous benefits or inappropriate assertions of evil can only delay the emergence of a nascent science. This paper is therefore a plea for moderation from both camps, lest we cause irreparable damage to our clinical practices and potential injury to individual patients. IEDs all contain arginine. However, they also contain other substances such as omega-3 fatty acids, and nucleotides. The use of all these nutrients together into commercial IEDs without adequate evaluation of their individual effects has prevented the development of mechanistic hypotheses of action. Despite this, IEDs have been tested extensively, allowing the development of guidelines for their use. IEDs should be used for surgical patients, especially those undergoing elective surgery. IEDs show no benefit and indeed can potentially harm patients with sepsis, especially in the nonsurgical group, and should not be used outside of research protocols. Advances in basic research have helped us understand mechanisms of how arginine contained in IEDs may help surgical patients but may be deleterious in patients with sepsis. A review of the basic mechanisms of action of arginine on the immune system is enclosed in this paper and should serve as a basis for the development of scientific principles that guide clinical use of IEDs.

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Role of nitric oxide in inflammation and tissue injury during endotoxemia and hemorrhagic shock.

Cited by 23 publications

References 44 publications

Effect of Lactobacillus plantarum and l-arginine against endotoxin-induced liver injury in a rat model

Effect of Lactobacillus plantarum and l-arginine against endotoxin-induced liver injury in a rat model

Insidious role of nitric oxide in migration/invasion of colon cancer cells by upregulating MMP-2/9 via activation of cGMP-PKG-ERK signaling pathways

A Rational Use of Immune Enhancing Diets: When Should We Use Dietary Arginine Supplementation?

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