Role of nitric oxide and prostaglandins in lipopolysaccharide-induced increase in vascular permeability in mouse skin

Fujii, Emiko; Irie, Koichiro; Ogawa, Akira; Ohba, Ken‐ichi; Muraki, Takamura

doi:10.1016/0014-2999(95)00758-x

Cited by 45 publications

(43 citation statements)

References 26 publications

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“…The e ects of drugs on LPS-induced dye leakage were evaluated at 2 h after topical application of LPS in this study, because we have previously shown that the dye leakage by LPS in the mouse skin reached maximum at 2 h (Fujii et al, 1996a). Pretreatment with pentoxifylline (160 mg kg 71 ), a non-selective PDE inhibitor, blocked the LPS-induced dye leakage ( Figure 1A).…”

Section: Effects Of Pde Inhibitors On Lps-induced Dye Leakagementioning

confidence: 96%

Inhibitory effects of cyclic AMP elevating agents on lipopolysaccharide (LPS)‐induced microvascular permeability change in mouse skin

Irie

Fujii

Ishida

et al. 2001

British J Pharmacology

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1 Anti-in¯ammatory e ects of cyclic AMP elevating agents were examined in a mouse model of lipopolysaccharide (LPS)-induced microvascular permeability change. 2 Vascular permeability on the back skin was measured by the local accumulation of Pontamine sky blue (PSB) after subcutaneous injection of LPS (400 mg site 71 ) from Salmonella typhimurium. 3 Dye leakage in the skin was signi®cantly increased 2 h after injection of LPS. This LPS-induced dye leakage was suppressed by phosphodiesterase inhibitors, including pentoxifylline (160 mg kg 71 ), milrinone (5 ± 10 mg kg 71 ), rolipram (0.5 ± 10 mg kg 71 ) and zaprinast (5 ± 10 mg kg 71 ). The dye leakage was also inhibited by b-adrenoceptor agonists, including isoproterenol (0.5 ± 5 mg kg 71 ) and salbutamol (0.05 ± 5 mg kg 71 ), an adenylate cyclase activator, forskolin (5 mg kg 71 ), and a cell permeable cyclic AMP analogue, 8-bromo-cyclic AMP (8-Br-cAMP, 10 mg kg 71 ). 4 LPS caused a transient increase in serum TNF-a level peaking at 1 h after the injection. This increase in serum TNF-a was completely blocked by a pretreatment with pentoxifylline (160 mg kg 71 ), milrinone (5 mg kg 71 ), rolipram (1 mg kg 71 ), zaprinast (10 mg kg 71 ), salbutamol (0.5 mg kg 71 ), forskolin (1 mg kg 71 ) and 8-Br-cAMP (10 mg kg 71 ). 5 LPS caused an increase in serum IL-1a level peaking at 3 h after injection. This increase in serum IL-1a was not signi®cantly suppressed by the cyclic AMP elevating agents. 6 Our study suggests that cyclic AMP elevating agents attenuate LPS-induced microvascular permeability change by suppressing TNF-a up regulation.

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Section: Effects Of Pde Inhibitors On Lps-induced Dye Leakagementioning

confidence: 96%

Inhibitory effects of cyclic AMP elevating agents on lipopolysaccharide (LPS)‐induced microvascular permeability change in mouse skin

Irie

Fujii

Ishida

et al. 2001

British J Pharmacology

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“…6,7) Hence, to further clarify the anti-inflammatory mechanism of BP radix and fruit at the dosage used in the carrageenan-induced edema experiment, we performed plasma leakage induced by autocrines, bradykinin, PAF, and SNP by Evan's blue extravasation in rats. The abdominal Evan's blue extravasation in the intradermally marked circle with saline was represented as 100%.…”

Section: Effects Of Bp Radix and Fruit On The Microvascular Permeabilmentioning

confidence: 99%

Comparison with Various Parts ofBroussonetia papyriferaas to the Antinociceptive and Anti-Inflammatory Activities in Rodents

Lin

Chen²,

Wu³

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…Previous reports have also suggested the role of prostaglandins in the regulation of vasopermeability and angiogenesis. 8 This study sought to evaluate the vitreous concentrations of haemodynamic (ET-1 and NO) and inflammatory (interleukin-1 b (IL-1 b) and prostacyclin) and anti-inflammatory (interleukin-1 receptor antagonist, IL-1 Ra) markers in primarily NPDR to ascertain if changes in these locally produced factors vary at this stage of retinopathy compared to PDR and also in patients with full thickness macular hole (FTMH).…”

Section: Introductionmentioning

confidence: 99%

Concentration of haemodynamic and inflammatory related cytokines in diabetic retinopathy

Patel

Saleh²,

Hykin

et al. 2006

Eye

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Aim There are changes in blood flow during the clinical stages of diabetic retinopathy with increasing leukostasis and secondary elaboration of cytokines. This study evaluated the vitreous concentrations of haemodynamicrelated (endothelin-1 (ET-1) and nitric oxide (NO)), inflammatory and anti-inflammatory (interleukin-1 receptor antagonist, IL-1 Ra) cytokines in the diabetic patients (with nonproliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR)), compared them with those of control patients (full thickness macular hole, FTMH) and correlated to macular structural indices. Method Vitreous samples from five FTMH patients representing normal controls were analysed together with the vitreous samples of 15 patients with NPDR and five with PDR. The vitreous concentrations of nitrite (total NO), ET-1, and prostacyclin was determined using ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. A sandwich luminescent immunoassay technique was used to determine IL-1b and IL-1 Ra concentrations. Results In the different clinical groups, there were no differences in the vitreous NO and prostacyclin concentrations. In NPDR, the median ET-1 concentration (0.7 pg/ml SD 70.8 pg/ml) was significantly reduced (Po0.05), compared to PDR (6.35 pg/ml SD 70.6 pg/ml) and FTMH (3.6 pg/ml SD 70.14 pg/ml). Its concentration also positively correlated with foveal thickness and macular volume (Po0.05) in patients with NPDR and macular oedema. IL-1 b was detected in PDR, and diabetic patients demonstrated a lower concentration of the anti-inflammatory cytokine IL-1 Ra.Conclusion Reduced concentrations of ET-1 in NPDR may reflect the haemodynamic changes of NPDR. The IL-1 Ra concentration suggests a change in the anti-inflammatory environment of the diabetic retina.

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Role of nitric oxide and prostaglandins in lipopolysaccharide-induced increase in vascular permeability in mouse skin

Cited by 45 publications

References 26 publications

Inhibitory effects of cyclic AMP elevating agents on lipopolysaccharide (LPS)‐induced microvascular permeability change in mouse skin

Inhibitory effects of cyclic AMP elevating agents on lipopolysaccharide (LPS)‐induced microvascular permeability change in mouse skin

Comparison with Various Parts ofBroussonetia papyriferaas to the Antinociceptive and Anti-Inflammatory Activities in Rodents

Concentration of haemodynamic and inflammatory related cytokines in diabetic retinopathy

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