Role of nanotopography in the development of tissue engineered 3D organs and tissues using mesenchymal stem cells

Salmasi, Shima; Kalaskar, Deepak M.; Yoon, Wai-Weng; Blunn, Gordon; Seifalian, Alexander M.

doi:10.4252/wjsc.v7.i2.266

Cited by 47 publications

(44 citation statements)

References 89 publications

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“…The emergence of 3-D cell culture platforms for tissue engineering applications [2, 26] will necessitate more sensitive analytical approaches to rapidly and accurately study, assess and profile biological processes within scaffolds [6]. In this study we have advanced the application of a high content image informatics approach to classify human mesenchymal stem cell (hMSC) differentiation on varied 3-D substrates.…”

Section: Discussionmentioning

confidence: 99%

Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics

et al. 2016

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A predictive framework for the evolution of stem cell biology in 3-D is currently lacking. In this study we propose deep image informatics of the nuclear biology of stem cells to elucidate how 3-D biomaterials steer stem cell lineage phenotypes. The approach is based on high content imaging informatics to capture minute variations in the 3-D spatial organization of splicing factor SC-35 in the nucleoplasm as a marker to classify emergent cell phenotypes of human mesenchymal stem cells (hMSCs). The cells were cultured in varied 3-D culture systems including hydrogels, electrospun mats and salt leached scaffolds. The approach encompasses high resolution 3-D imaging of SC-35 domains and high content image analysis (HCIA) to compute quantitative 3-D nuclear metrics for SC-35 organization in single cells in concert with machine learning approaches to construct a predictive cell-state classification model. Our findings indicate that hMSCs cultured in collagen hydrogels and induced to differentiate into osteogenic or adipogenic lineages could be classified into the three lineages (stem, adipogenic, osteogenic) with ≥ 80% precision and sensitivity, within 72 hours. Using this framework, the augmentation of osteogenesis by scaffold design exerted by porogen leached scaffolds was also profiled within 72 hours with ~80% high sensitivity. Furthermore, by employing 3-D SC-35 organizational metrics, differential osteogenesis induced by novel electrospun fibrous polymer mats incorporating decellularized matrix could also be elucidated and predictably modeled at just 3 days with high precision. We demonstrate that 3-D SC-35 organizational metrics can be applied to model the stem cell state in 3-D scaffolds. We propose that this methodology can robustly discern minute changes in stem cell states within complex 3-D architectures and map single cell biological readouts that are critical to assessing population level cell heterogeneity.

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Section: Discussionmentioning

confidence: 99%

Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics

et al. 2016

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“…Organ development have been based on a 3D scaffold, made either from non-biodegradable materials or bio absorbable materials which are coated with stem cells in a bioreactor [5]. There are numerous benefits of using autologous stem cells for coating, as there is no need for severe immune suppression.…”

Section: Development Of Organs Using Stem Cellsmentioning

confidence: 99%

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2016

JSRT

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“…87 Mesenchymal stem cells are used for development of laboratory based 3D organs and tissues. 14 However, in regeneration and tissue engineering both mesenchymal stem cell (MSC) therapy and a combination therapy of MSCs transfected with vascular endothelial growth factor (VEGF) are used for liver regeneration after major resection. 88 Transplanted stem cells and MSCs transfected with VEGF significantly accelerate the healing process following major hepatic resection.…”

Section: Use Of Tissue Specific Stem Cellsmentioning

confidence: 99%

“…Nanotopography put impact on mesenchymal stem cells and assists in developing laboratory-based 3D organs and tissues. 14 Regenerative medicine offer novel strategies to treat patients with endstage organ failure. Further, new advancements in tissue technology are waiting to replace aged and genetically defective organs.…”

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confidence: 99%

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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Present review article emphasizes role of biological scaffolds, hydrogels and stem cells in tissue engineering mainly in regeneration or repairing of damaged tissues. Highly porous scaffold biomaterials are developed which act as templates for tissue regeneration and potentially guide the growth of new tissue. Present article also describes de-cellularization, cell printing, vascularization, integration of cross linking of methods for scaffolding of biomaterials to reproduce and recapitulate complexity in engineered tissues. It also explains different scaffold types, polymer hydrogels which are necessary for formation of microstructure, cell attachment, differentiation, tissue vascularization and integration. It also explains use of induced pluripotent stem cells (iPSCs) and engineered MSCs as new diagnostic and potential therapeutic tools to remove sustained damage and complications from organ failures. These engineered MSCs assist in making self-assembling supramolecular hydrogels, which have larger applications in cell therapy of intractable diseases and tissue regeneration. No doubt development of more advanced biomaterials, growth factors and stem cell derived products/factors and tissue transplantation methods based on cell regeneration programming will revolutionize the clinical therapeutics. This article suggests identification of new biomaterials/biomolecules such as growth factors, scaffolds, integration, adhesion and regulatory molecules and cell secreted factors which can induce major metabolic and signaling pathways during phase of tissue repairing and induction of regeneration. This innovative research area needs many conceptual improvements in organ therapies, methods and technological advancement to scale more services to the human society.

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Role of nanotopography in the development of tissue engineered 3D organs and tissues using mesenchymal stem cells

Cited by 47 publications

References 89 publications

Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics

Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics

Untitled

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

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