Role of NADPHox/Rho-kinase signaling in the cyclosporine-NSAIDs interactions on blood pressure and baroreflexes in female rats

El‐Gowelli, Hanan M.; Ibrahim, Karim; El-Yazbi, Ahmed F.; El‐Mas, Mahmoud M.

doi:10.1016/j.lfs.2017.07.019

Cited by 13 publications

(6 citation statements)

References 51 publications

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“…Rats were allowed to stabilize for 45 min before the experiment. Baroreceptor sensitivity (BRS), as an indicator of CAN, was assessed by the vasoactive method as previously described (18). This is a common and reliable method for the assessment of BRS (25,32,54) that was not only validated to represent cardiac parasympathetic and sympathetic activities using the corresponding blockers (57), but also correlated quite closely with peripheral sympathetic nerve firing activity (28).…”

Section: Methodsmentioning

confidence: 99%

Worsening baroreflex sensitivity on progression to type 2 diabetes: localized vs. systemic inflammation and role of antidiabetic therapy

Bakkar

Mougharbil

Mroueh

et al. 2020

American Journal of Physiology-Endocrinology and Metabolism

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Cardiac autonomic neuropathy (CAN) is an early cardiovascular manifestation of type 2 diabetes (T2D) that constitutes an independent risk factor for cardiovascular mortality and morbidity. Nevertheless, its underlying pathophysiology remains poorly understood. We recently showed that localized perivascular adipose tissue (PVAT) inflammation underlies the incidence of parasympathetic CAN in prediabetes. Here, we extend our investigation to provide a mechanistic framework for the evolution of autonomic impairment as the metabolic insult worsens. Early metabolic dysfunction was induced in rats fed a mild hypercaloric diet. Two low-dose streptozotocin injections were used to evoke a state of late decompensated T2D. Cardiac autonomic function was assessed by invasive measurement of baroreflex sensitivity using the vasoactive method. Progression into T2D was associated with aggravation of CAN to include both sympathetic and parasympathetic arms. Unlike prediabetic rats, T2D rats showed markers of brainstem neuronal injury and inflammation as well as increased serum levels of IL-1b. Experiments on PC12 cells differentiated into sympathetic-like neurons demonstrated that brainstem injury observed in T2D rats resulted from exposure to possible pro-inflammatory mediators in rat serum rather than a direct effect of the altered metabolic profile. CAN and the associated cardiovascular damage in T2D only responded to combined treatment with insulin to manage hyperglycemia in addition to a non-hypoglycemic dose of metformin or pioglitazone providing an anti-inflammatory effect, coincident with the effect of these combinations on serum IL-1b. Our present results indicate that CAN worsening upon progression to T2D involves brainstem inflammatory changes likely triggered by systemic inflammation.

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Section: Methodsmentioning

confidence: 99%

Worsening baroreflex sensitivity on progression to type 2 diabetes: localized vs. systemic inflammation and role of antidiabetic therapy

Bakkar

Mougharbil

Mroueh

et al. 2020

American Journal of Physiology-Endocrinology and Metabolism

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“…Baroreceptor sensitivity (BRS) was assessed using the vasoactive method [ 32 ]. Increasing doses of phenylephrine (PE, 0.25, 0.5, 0.75, 1, and 2 μ g) and sodium nitroprusside (SNP, 1, 2, 4, and 8 μ g) were administered, and the changes in MAP and HR were recorded.…”

Section: Methodsmentioning

confidence: 99%

Cardiac Autonomic Neuropathy as a Result of Mild Hypercaloric Challenge in Absence of Signs of Diabetes: Modulation by Antidiabetic Drugs

Al-Assi

Ghali

Mroueh

et al. 2018

Oxidative Medicine and Cellular Longevity

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Cardiac autonomic neuropathy (CAN) is an early cardiovascular complication of diabetes occurring before metabolic derangement is evident. The cause of CAN remains elusive and cannot be directly linked to hyperglycemia. Recent clinical data report cardioprotective effects of some antidiabetic drugs independent of their hypoglycemic action. Here, we used a rat model receiving limited daily increase in calories from fat (HC diet) to assess whether mild metabolic challenge led to CAN in absence of interfering effects of hyperglycemia, glucose intolerance, or obesity. Rats receiving HC diet for 12 weeks showed reduction in baroreceptor sensitivity and heart rate variability despite lack of change in baseline hemodynamic and cardiovascular structural parameters. Impairment of cardiac autonomic control was accompanied with perivascular adipose inflammation observed as an increased inflammatory cytokine expression, together with increased cardiac oxidative stress, and signaling derangement characteristic of diabetic cardiomyopathy. Two-week treatment with metformin or pioglitazone rectified the autonomic derangement and corrected the molecular changes. Switching rats to normal chow but not to isocaloric amounts of HC for two weeks reversed CAN. As such, we conclude that adipose inflammation due to increased fat intake might underlie development of CAN and, hence, the beneficial effects of metformin and pioglitazone.

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“…The analysis was performed using GraphPad Prism, software release 3.02. Probability levels less than 0.05 were considered significant (El-Gowelli et al 2017). Herbal mixtures that are used by Saudi women, in North region, as a treatment for difficulty of pregnancy were recorded.…”

Section: Methodsmentioning

confidence: 99%

Insight in some herbals and herbal combinations used by Saudi females for improving fertility and enhance pregnancy

Zaatout¹

2019

Records of Pharmaceutical and Biomedical Sciences

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Delayed pregnancy is one of the main problems that may threaten the relationship between married people especially in Arab region. Finding a cure for this problem started after a period of time after the marriage without pregnancy. In some cases, herbs from tradition can be used in order to promote fertility and stimulate pregnancy. There are limited data for herbal remedies used by women in KSA that is concerned with pregnancy. This study was aimed at determining the prevalence rate and kind of herbal remedies used among female culture. First, a pre-structured questionnaire was administered among 430 Saudi females which revealed that 25% of them have used traditional herbals as a tool for enhancing the fertility. Then asking apothecary and dealers in that field, collecting samples and data were recorded. Finally, investigating the data collected in attempts to evaluate the efficacy and safety of such herbs and herbal mixtures. Rose of Jericho, Juniper, Myrrh, Black cumin and garden cress were the most commonly used remedies recorded from respondents. This study focuses on possible warning and precaution. Recommendations were suggested to control any hazards.

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Role of NADPHox/Rho-kinase signaling in the cyclosporine-NSAIDs interactions on blood pressure and baroreflexes in female rats

Cited by 13 publications

References 51 publications

Worsening baroreflex sensitivity on progression to type 2 diabetes: localized vs. systemic inflammation and role of antidiabetic therapy

Worsening baroreflex sensitivity on progression to type 2 diabetes: localized vs. systemic inflammation and role of antidiabetic therapy

Cardiac Autonomic Neuropathy as a Result of Mild Hypercaloric Challenge in Absence of Signs of Diabetes: Modulation by Antidiabetic Drugs

Insight in some herbals and herbal combinations used by Saudi females for improving fertility and enhance pregnancy

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