Abstract:Amrinone is a bipyridine drug with characteristic effects on the force-velocity relationship of fast skeletal muscle. Here we combined in vitro motility assays, transient biochemical kinetics and optical tweezers studies to elucidate the mechanisms underlying the drug effects. Amrinone (1-2 mM) reduced the sliding velocity of heavy meromyosin (HMM) propelled actin filaments by 31.0 5 2.5% (n ¼ 15) at different ionic strengths of the assay solution (20 -160 mM). The drug also reduced (by 2 -18%) the sliding vel… Show more
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