Role of MUC1 and MUC5AC expressions as prognostic indicators in gastric carcinomas

Wang, Jaw‐Yuan; Chang, Chun‐Chi; Hsieh, Jan-Sing; Lee, Li‐Wei; Huang, Tsung-Jen; Chai, Chee‐Yin; Lin, Shiu‐Ru

doi:10.1002/jso.10222

Cited by 36 publications

(35 citation statements)

References 28 publications

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“…Our studies revealed high MUC1 levels in endometrial adenocarcinomas and cervical carcinomas when compared to the normal endometrial and cervical tissues. These results are in accord with studies where MUC1 up-regulation was reported in variety of carcinomas including pancreas[42], breast[43], stomach[44], colon rectum [45] and lung [46]. …”

Section: Discussionsupporting

confidence: 92%

Differential expression of MUC genes in endometrial and cervical tissues and tumors

2005

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Background: Mucin glycoprotein's are major components of mucus and are considered an important class of tumor associated antigens. The objective of this study was to investigate the expression of human MUC genes (MUC1, MUC2, MUC5B, MUC5AC and MUC8) in human endometrium and cervix, and to compare and quantitate the expression of MUC genes in normal and cancerous tissues.Methods: Slot blot techniques were used to study the MUC gene expression and quantitation.Results: Of the five-mucin genes studied, MUC1, MUC5B and MUC8 showed high expression levels in the normal and cancerous endometrial and cervical tissues, MUC2 and MUC5AC showed considerably lower expression. Statistically, higher levels of MUC1, MUC5B and MUC8 were observed in endometrial adenocarcinomas compared to normal tissues. In contrast, only MUC1 levels increased with no significant changes in expression of MUC5B and MUC8 in cervical tumors over normal cervical tissues. Conclusion:Endometrial tumors showed increased expression of MUC1, MUC5B and MUC8 over normal tissues. Only MUC1 appears to be increase, in cervical tumors. All the studied tissues showed high and consistent expression of MUC8 mRNA. Low to neglible levels of MUC2 and MUC5AC were observed in all studied endometrial and cervical tissues.

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Section: Discussionsupporting

confidence: 92%

Differential expression of MUC genes in endometrial and cervical tissues and tumors

2005

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“…For example, Colon Cancer Related Gene, a growth factor encoding a novel cysteine-rich motif with preferential expression in majority of colon tumors, was found by using a data-mining tool called Digital Differential Display to search the EST databases (29). Of our electronic gene expression profiling, a number of genes have been previously proven to be involved in gastric cancer from literatures, such as mucin 5B (30), prothymosin a (31), CDX1 (32), Wnt7B (33), syndecan1 (34), mucin1 (35), and HMGA1 (36), which might confirm the validity of digital gene expression profiling of gastric cancer based on public databases. Recently, we have confirmed these data, including MMP11overexpression of gastric cancer in our following gastric cancer profiling by Oligo microarray analysis (data not shown).…”

Section: Discussionsupporting

confidence: 69%

Identification of Matrix Metalloproteinase 11 as a Predictive Tumor Marker in Serum Based on Gene Expression Profiling

Yang

Deng

et al. 2008

Clinical Cancer Research

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Purpose: Prognostic markers discovery is a strategy for early diagnosis and individualization therapy for human cancer. In this study, we focus to integrate different methods to identify specific biomarker and elucidate its clinical significance. Experimental Design: A powerful tool named Digital Gene Expression Display online was applied to isolate differentially expressed genes correlated with gastric cancer. Matrix metalloproteinase 11 (MMP11) was selected and confirmed at both mRNA and protein level in 10 cell lines, 123 cases of tumor tissues, and 305 cases of gastric cancer serum specimen by semiquantitative PCR, immunohistochemistry staining, and ELISA techniques, respectively. Results: Our data showed that overexpression of MMP11 at mRNA and protein level was consistently detected in cell lines and primary tumors compared with matched normal tissues. Importantly, serum MMP11 levels were also significantly elevated in gastric cancer patients compared with those of the control subjects (P < 0.001), and the positive expression was well correlated with metastasis in gastric cancer patients (P = 0.009). Furthermore, we have shown that overexpression of MMP11was associated with the malignant proliferation of AGS cells. Conclusions: Combination of gene expression profiling and specific clinical resource is a promising approach to validate gene expression patterns associated with malignant phenotype. As a secreted protein, MMP11 may play an important role in carcinogenesis and has potential implication as a biomarker for the diagnosis and prognosis of human cancers including gastric cancer.

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“…However, conflicting data about the prognostic significance of Muc5AC have been reported: several investigators have reported that Muc5AC expression is a good prognostic indicator in gastric, gallbladder and colon cancer, whereas others have reported that it is a poor prognostic indicator in pancreatic cancer . Conflicting results have also been published on the clinical and prognostic significance of Muc5AC in gastric cancer …”

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confidence: 99%

Decreased Muc5AC expression is associated with poor prognosis in gastric cancer

Kim

Kwon

Shin

et al. 2013

Intl Journal of Cancer

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Mucins reportedly play numerous key roles in carcinogenesis, including in tumor invasion, regulation of differentiation and tumor cell proliferation. We investigated the effect of Muc5AC, a secreted mucin, on the invasiveness=migratory capability of gastric cancer cells and the prognostic significance of Muc5AC in gastric cancer patients. The clinicopathological and prognostic significance of Muc5AC expression was validated using immunohistochemical analysis in 412 gastric cancer patients. Differential gene expression was investigated using complementary DNA microarray analysis of 48 fresh tumor tissue samples. Silencing of Muc5AC by using a small hairpin RNA-containing lentivirus increased the invasion and migration of SNU216 and AGS cells as well as Akt phosphorylation and the expression of vascular endothelial growth factor and matrix metalloproteinase-7, which were blocked by inhibitors of the phosphatidylinositol 3-kinase=Akt pathway. Loss of Muc5AC expression was significantly associated with tumor progression (advanced T stage; p 5 0.004), lymph node metastases (p 5 0.001), lymphovascular invasion (p < 0.0001), and increased tumor size (p 5 0.027). Lower MUC5AC expression was identified as an independent poor prognostic factor in diffuse-type gastric cancer by using the Cox regression proportional hazard model (hazard ratio, 2.39; p 5 0.043). Complementary DNA microarray analysis revealed 86 differentially expressed genes, including genes related to metastasis and invasion, in gastric cancer tissues with high (25%) and low (<25%) Muc5AC expression levels. Low Muc5AC expression increased the invasion and migration of gastric cancer cells and could be a useful biomarker of poor prognosis in gastric cancer.Mucins are a group of diverse, complex, highly glycosylated extracellular proteins that are major components of the mucus gel covering the gastric mucosa.

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Role of MUC1 and MUC5AC expressions as prognostic indicators in gastric carcinomas

Abstract: Altogether these data suggest that combined evaluation of MUC1 and MUC5AC mucin staining may be clinically helpful to predict outcome in patients with gastric cancer.

Cited by 36 publications

References 28 publications

Differential expression of MUC genes in endometrial and cervical tissues and tumors

Differential expression of MUC genes in endometrial and cervical tissues and tumors

Identification of Matrix Metalloproteinase 11 as a Predictive Tumor Marker in Serum Based on Gene Expression Profiling

Decreased Muc5AC expression is associated with poor prognosis in gastric cancer

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