Role of mononuclear stem cells and decellularized amniotic membrane in the treatment of skin wounds in rats

Takejima, Aline; Francisco, Júlio César; Simeoni, Rossana Baggio; Noronha, Lúcia de; Garbers, Luiz A.F.M.; Foltz, Kátia Martins; Machado, Paulo A.B.; Souza, Isio Carvalho de; Pinho, Ricardo A.; Carvalho, Katherine Athayde Teixeira de

doi:10.1080/21688370.2021.1982364

Cited by 3 publications

(4 citation statements)

References 40 publications

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“…The cell harvesting was achieved by the puncture and aspiration of the posterior iliac crest from the same animals, using 5-mL syringes containing 0.2 mL of heparin (5.000 UI/mL). After puncturing the iliac crest, the collected bone marrow blood 0,5 to 1,0mL was transferred to a 15 mL flask and mixed with sterile phosphate-buffered saline (PBS) (Boyum 1968, Takejima et al 2021.…”

Section: Methodsmentioning

confidence: 99%

Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure

TAKEJIMA,

MACHADO-JÚNIOR,

BLUME

et al. 2024

An. Acad. Bras. Ciênc.

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Recent studies have suggested that therapies with stem cells and amniotic membrane can modulate the inflammation following an ischemic injury in the heart.This study evaluated the effects of bone-marrow mononuclear cells (BMMC) and acellular human amniotic membrane (AHAM) on cardiac function and NLRP3 complex in a rat model of heart failure.On the 30th day,the echocardiographic showed improvements on ejection fraction and decreased pathological ventricular remodeling on BMMC and AHAM groups.Oxidative stress analysis was similar between the three groups,and the NLRP3 inflammasome activity were not decreased with the therapeutic use of both BMMC and AHAM,in comparison to the control group.

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Section: Methodsmentioning

confidence: 99%

Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure

TAKEJIMA,

MACHADO-JÚNIOR,

BLUME

et al. 2024

An. Acad. Bras. Ciênc.

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“…After aspiration and washing, 90 µL of substrate solution was added to wells and incubated for 15 min at 37 °C. The optical density (OD) of the reaction product was read on a microplate reader (Sigma-Aldrich, St. Louis, MO, USA) at 450 nm [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Protein carbonylation was determined according to Levine et al [ 15 ]. Briefly, carbonyl group levels were estimated spectrophotometrically at 412 nm using 2,4-dinitrophenylhydrazine (DTNB), and the amounts of TNB formed (equivalent to the amounts of carbonyl were calculated).…”

Section: Methodsmentioning

confidence: 99%

Acellular Biomaterials Associated with Autologous Bone Marrow-Derived Mononuclear Stem Cells Improve Wound Healing through Paracrine Effects

et al. 2023

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Wound healing is a complex process of repair that involves the interaction between different cell types and involves coordinated interactions between intracellular and extracellular signaling. Bone Marrow Mesenchymal Stem Cells (BMSCs) based and acellular amniotic membrane (AM) therapeutic strategies with the potential for treatment and regeneration of tissue. We aimed to evaluate the involvement of paracrine effects in tissue repair after the flap skin lesion rat model. In the full-thickness flap skin experiment of forty Wistar rats: A total of 40 male Wistar rats were randomized into four groups: group I: control (C; n = 10), with full-thickness lesions on the back, without (BMSCs) or AM (n = 10); group II: injected (BMSCs; n = 10); group III: covered by AM; group IV–injected (AM + BMSCs; n = 10). Cytokine levels, IL-1, and IL-10 assay kits, superoxide dismutase (SOD), glutathione reductase (GRs) and carbonyl activity levels were measured by ELISA 28th day, and TGF-β was evaluated by immunohistochemical, the expression collagen expression was evaluated by Picrosirius staining. Our results showed that the IL-1 interleukin was higher in the control group, and the IL-10 presented a higher mean when compared to the control group. The groups with BMSCs and AM showed the lowest expression levels of TGF-β. SOD, GRs, and carbonyl activity analysis showed a predominance in groups that received treatment from 80%. The collagen fiber type I was predominant in all groups; however, the AM + BMSCs group obtained a higher average when compared to the control group. Our findings suggest that the AM+ BMSCs promote skin wound healing, probably owing to their paracrine effect attributed to the promotion of new collagen for tissue repair.

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“…The amniotic membrane (AM) is suitable for the development of biomaterials because it has factors that allow adequate regeneration and healing of lesions (Lacorzana 2020, Takejima et al 2021. The application of AM has been studied in the regeneration of various systems including ocular, nervous and pericardial tissues.…”

Section: Introductionmentioning

confidence: 99%

Tracheal regeneration with acellular human amniotic membrane and 15-deoxy-∆12,14prostaglandinj2 nanoparticles in a rabbit model

MIGGIOLARO,

TAKEJIMA,

FRANCISCO

et al. 2023

An. Acad. Bras. Ciênc.

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The treatment of tracheal pathologies remains challenging.Nanotechnology allows adding substances to decellularized human amniotic membrane (DHAM), such as 15-Deoxy-∆12,14ProstaglandinJ2 nanoparticles (15D-PGJ2-NC).This study performed a tracheotomy in rabbits randomized into three groups.The tissue repair process was evaluated when treated with DHAM associated or not with 15D-PGJ2-NC.The average of the area in the control group was 54.76% smaller than DHAM group and 41.98% smaller than DHAM + 15D-PGJ2-NC group (p=0.004 for both).The DHAM + 15D-PGJ2-NC group had significantly more immature cartilage (p=0.015).DHAM impregnated with 15D-PGJ2-NC could provide support for the healing of the tracheal defect and may prevent reduction of its lumen.

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Role of mononuclear stem cells and decellularized amniotic membrane in the treatment of skin wounds in rats

Cited by 3 publications

References 40 publications

Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure

Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure

Acellular Biomaterials Associated with Autologous Bone Marrow-Derived Mononuclear Stem Cells Improve Wound Healing through Paracrine Effects

Tracheal regeneration with acellular human amniotic membrane and 15-deoxy-∆12,14prostaglandinj2 nanoparticles in a rabbit model

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