Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa

Abstract

The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds. In the current study, cryoprotective media with a combined composition of mono- and di-saccharides were developed. The degree of penetration of reducing saccharide molecules (maltose—Mal20 medium) and non-reducing disaccharide molecules (trehalose—Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied. LCM control media without disaccharides were used as the control. The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104. Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively). A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05). Maltose positively affected the preservation of frozen/thawed sperm in the genital tract of hens. On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20. Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.

Influence of Technological Stages of Preparation of Rooster Semen for Short-Term and Long-Term Storage on Its Quality Characteristics

“…It has been established that impaired fertility of rooster spermatozoa may be associated with a change in the content of the carbohydrate components of their glycocalyx [ 6 ]. The role of the surface carbohydrates of rooster spermatozoa in maintaining their fertility during short-term and long-term storage is well-covered in the literature [ 14 , 15 ]. However, no less important is the study of the composition of the cytosol of spermatozoa and its changes under the influence of technological factors.…”

Screening and Analysis of Key Transcription Factors of Yili Goose Sperm Motility miRNAs-mRNAs

“…Sperm can also metabolize fructose through the glycolytic pathway. Fructose is abundant in poultry seminal plasma and plays a crucial role in maintaining sperm motility and seminal plasma osmotic pressure (Stanishevskaya et al, 2021;Goodson et al, 2012). The above studies suggest that amino acids participate in the cell metabolism of motile sperm, are energy supplement sources, and can enhance sperm activity.…”

Short Communication: The use of lactose-astaxanthin to maintain the quality of green junglefowl frozen semen

“…The role of lactose or other types of carbohydrates in semen storage is to reduce damage to cell plasma membranes during the process of cooling, freezing, and thawing (Bebas et al 2018). Therefore, carbohydrates increase the fluidity of the plasma membrane before freezing (Stanishevskaya et al 2021). Stanishevskaya et al (2021a), as well as Bebas and Laksmi (2015), stated that lactose plays a role in replacing the water position on the surface of the cell plasma membrane, which is directly related to the diluent.…”

“…It can interact directly with the polar phospholipid central group during freezing and reduce the interaction of van der Walls bonds between the carbon chains (Iqbal et al 2018). Meanwhile, adding carbohydrates, either monosaccharides or disaccharides, to the diluent for semen freezing is helpful as an extracellular cryoprotectant essential in maintaining membrane flexibility (Athurupana et al 2015;Stanishevskaya et al 2021;Stanishevskaya et al 2021a).…”