Role of monensin PLGA polymer nanoparticles and liposomes as potentiator of ricin A immunotoxins in vitro

Ferdous, Afia; Stembridge, Neena Y; Singh, Mandip

doi:10.1016/s0168-3659(97)00116-8

Cited by 44 publications

(30 citation statements)

References 15 publications

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“…Outra técnica que tem sido empregada para caracterizar a morfologia de superfície das nanopartículas é a microscopia de força atômica [69][70][71][72] , a qual fornece informações com alta resolução em três dimensões, em escala nanométrica, sendo capaz ainda de resolver detalhes de superfície em nível atômico 72 . Feng e colaboradores 69 empregaram esta técnica visando um estudo morfológico mais detalhado de nanoesferas de PLGA, preparadas empregando-se diferentes emulsificantes.…”

Section: Avaliação Morfológicaunclassified

Caracterização e estabilidade físico-química de sistemas poliméricos nanoparticulados para administração de fármacos

et al. 2003

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Recebido em 28/10/02; aceito em 27/2/03 PHYSICOCHEMICAL CHARACTERIZATION AND STABILITY OF THE POLYMERIC NANOPARTICLE SYSTEMS FOR DRUG ADMINISTRATION. Polymeric nanoparticle systems such as nanocapsules and nanospheres present potential applications for the administration of therapeutic molecules. The physico-chemical characteristics of nanoparticle suspensions are important pre-requisites of the success of any dosage form development. The purpose of this review is to present the state of the art regarding the physico-chemical characterization of these drug carriers, in terms of the particle size distribution, the morphology, the polymer molecular weight, the surface charge, the drug content and the in vitro drug release profiles. Part of the review is devoted to the description of the techniques to improve the stability of colloidal systems.Keywords: nanoparticles; polymers; characterization. INTRODUÇÃOO controle da liberação de fármacos em sítios de ação específi-cos, através da utilização de vetores, capazes de permitir a otimização da velocidade de cedência e do regime de dosagem das substâncias, tem sido uma área de intensa pesquisa nos últimos dez anos. Dentre os vetores, incluem-se as micropartículas e os sistemas coloidais (lipossomas e nanopartículas). As nanopartículas, constituídas por polímeros biodegradáveis, têm atraído maior atenção dos pesquisadores em relação aos lipossomas, devido às suas potencialidades terapêuticas, à maior estabilidade nos fluídos biológicos e durante o armazenamento 1-3 . As nanopartículas poliméricas são sistemas carreadores de fármacos que apresentam diâmetro inferior a 1 µm. O termo nanopartícula inclui as nanocápsulas e as nanoesferas, as quais diferem entre si segundo a composição e organização estrutural (Figura 1). As nanocápsulas são constituídas por um invólucro polimérico disposto ao redor de um núcleo oleoso, podendo o fármaco estar dissolvido neste núcleo e/ou adsorvido à parede polimérica. Por outro lado, as nanoesferas, que não apresentam óleo em sua composição, são formadas por uma matriz polimérica, onde o fármaco pode ficar retido ou adsorvido 2,4,5 . Estes sistemas têm sido desenvolvidos visando inúmeras aplicações terapêuticas, sendo planejados, principalmente, para administração parenteral, oral ou oftálmica. Uma das áreas mais promissoras na utilização das nanopartículas é a vetorização de fármacos anticancerígenos 2,6-8 e de antibióticos 2,9,10 , principalmente através de administração parenteral, almejando uma distribuição mais seletiva dos mesmos e, assim, um aumento do índice terapêutico. Com relação à administração oral de nanopartículas, as pesquisas têm sido direcionadas especialmente à: a) diminuição dos efeitos colaterais de certos fármacos, destacando-se os antiinflamatórios não-esteróides (diclofenaco 11,12 , indometacina 13-15 ), os quais causam freqüentemente irritação à mucosa gastrintestinal e b) proteção de fármacos degradáveis no trato gastrintestinal, como peptídeos 16,17 , proteínas 8,18 e/ou hormônios 19 , aumentando a biodisponi...

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Section: Avaliação Morfológicaunclassified

Caracterização e estabilidade físico-química de sistemas poliméricos nanoparticulados para administração de fármacos

et al. 2003

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“…The similar result was reported by previous article. 29 BSA EE% was increasing with the increase of the concentration of PVA. The reason was that tight surface was formed from PVA macromolecules of high concentration, which increased diffusion resistance of BSA from the internal aqueous phase and stabilized the emulsion.…”

Section: De Techniquementioning

confidence: 87%

Synthesis and characterization of an amphiphilic hyperbranched poly(amine‐ester)‐co‐D,L‐lactide (HPAE‐co‐PLA) copolymers and their nanoparticles for protein drug delivery

Jiang

et al. 2010

J of Applied Polymer Sci

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A series of hyperbranched poly(amineester)-co-D,L-lactide (HPAE-co-PLA) copolymer were synthesized by ring-opening polymerization of D,L-lactide with Sn(Oct) 2 as catalyst to a fourth generation branched poly(amine-ester) (HPAE-OHs4). The chemical structures of copolymers were determined by FTIR, 1 H-NMR, 13C-NMR, and TGA. Double emulsion (DE) and nanoprecipitation (NP) method were used to fabricate the nanoparticles of these copolymers encapsulating bovine serum albumin (BSA) as a model. DSC thermo-grams indicated that the nanoparticles with BSA kept stable below 40 C. Different factors which influence on particular size and encapsulation efficiency (EE) were investigated. Their EE to BSA could reach 97.8% at an available condition. In vitro release behavior of NPs showed a continuous release after a burst release. The stability maintenance of BSA in the nanoparticle release in vitro was also measured via circular dichroism and fluorescence spectrometry. The results showed that the copolymer nanoparticles have a promising potential in protein delivery system.

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“…Zeta potential is generally dependant on amount of drug, polymer used, surfactant and coating agent used. For example, Chitosan nanoparticles [31], and gelatin nanoparticles [32] are positively charged, whereas poly capro-lactone nanoparticles [33], PLGA nanoparticles [34] have a negative charge. The presence of the ionized carboxylic acid groups at the surface are responsible for this negative charge on the PLGA nanoparticles [35].…”

Section: Zeta Potentialmentioning

confidence: 99%

Current Analytical Methods Used in the In Vitro Evaluation of Nano-Drug Delivery Systems

Nagvekar¹,

Trickler²,

Dash³

2009

CPA

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Nano-Drug Delivery Systems have emerged as an effective means of targeting therapeutic agents such as drugs, peptides and proteins to various target sites. The unique advantages and potential targeting capability of this approach have claimed it as the drug delivery system of the future. Various analytical methodologies have been used for the evaluation of this delivery system both in vitro and in vivo. This review will attempt to describe the various in vitro methods used for this purpose, their importance and limitations. The in vitro methods include particle sizing, zeta potential, microscopy, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry, X-ray diffraction, in vitro release characteristics, determination of drug loading, encapsulation efficiency and loading efficiency. In vitro cell culture has also been utilized in cellular uptake, transmission electron microscopy, MTT assays, flow cytometry, and cellular transport studies. In summary, this in-depth review will provide the basic understanding, as well as the pros and cons of commonly used analytical methods currently utilized to evaluate this emerging drug delivery system.

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Role of monensin PLGA polymer nanoparticles and liposomes as potentiator of ricin A immunotoxins in vitro

Cited by 44 publications

References 15 publications

Caracterização e estabilidade físico-química de sistemas poliméricos nanoparticulados para administração de fármacos

Caracterização e estabilidade físico-química de sistemas poliméricos nanoparticulados para administração de fármacos

Synthesis and characterization of an amphiphilic hyperbranched poly(amine‐ester)‐co‐D,L‐lactide (HPAE‐co‐PLA) copolymers and their nanoparticles for protein drug delivery

Current Analytical Methods Used in the In Vitro Evaluation of Nano-Drug Delivery Systems

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