Role of mobile introns in mitochondrial genome diversity of fungi

Hamari, Zsuzsanna; Juhász, Ákos; Kevei, Ferenc

doi:10.1556/amicr.49.2002.2-3.22

Cited by 27 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By contrast, Aspergillus japonicus , P . anserina , Neurospora crassa and some other fungi exhibit significant mitochondrial intraspecies polymorphism and genome size variation, which has been attributed to mobile introns [11]. Thus, population surveys based on RFLP have demonstrated the presence of different mitochondrial haplotypes in wild-type subpopulations of P .…”

Section: Resultsmentioning

confidence: 99%

“…The widespread uniparental inheritance and high copy number of these organelles make them promising markers for cost-effective species identification and for studying fungal population structure [10]. Mitochondrial DNA can be a rich source of novel genotyping markers due to the presence of highly mobile introns in many fungal mitochondria [11]. Finally, fungal mitochondria may serve as valuable experimental models for studies of human heart and muscle diseases linked to mitochondrial dysfunction [12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability

et al. 2012

View full text Add to dashboard Cite

BackgroundThe genera Aspergillus and Penicillium include some of the most beneficial as well as the most harmful fungal species such as the penicillin-producer Penicillium chrysogenum and the human pathogen Aspergillus fumigatus, respectively. Their mitochondrial genomic sequences may hold vital clues into the mechanisms of their evolution, population genetics, and biology, yet only a handful of these genomes have been fully sequenced and annotated.ResultsHere we report the complete sequence and annotation of the mitochondrial genomes of six Aspergillus and three Penicillium species: A. fumigatus, A. clavatus, A. oryzae, A. flavus, Neosartorya fischeri (A. fischerianus), A. terreus, P. chrysogenum, P. marneffei, and Talaromyces stipitatus (P. stipitatum). The accompanying comparative analysis of these and related publicly available mitochondrial genomes reveals wide variation in size (25–36 Kb) among these closely related fungi. The sources of genome expansion include group I introns and accessory genes encoding putative homing endonucleases, DNA and RNA polymerases (presumed to be of plasmid origin) and hypothetical proteins. The two smallest sequenced genomes (A. terreus and P. chrysogenum) do not contain introns in protein-coding genes, whereas the largest genome (T. stipitatus), contains a total of eleven introns. All of the sequenced genomes have a group I intron in the large ribosomal subunit RNA gene, suggesting that this intron is fixed in these species. Subsequent analysis of several A. fumigatus strains showed low intraspecies variation. This study also includes a phylogenetic analysis based on 14 concatenated core mitochondrial proteins. The phylogenetic tree has a different topology from published multilocus trees, highlighting the challenges still facing the Aspergillus systematics.ConclusionsThe study expands the genomic resources available to fungal biologists by providing mitochondrial genomes with consistent annotations for future genetic, evolutionary and population studies. Despite the conservation of the core genes, the mitochondrial genomes of Aspergillus and Penicillium species examined here exhibit significant amount of interspecies variation. Most of this variation can be attributed to accessory genes and mobile introns, presumably acquired by horizontal gene transfer of mitochondrial plasmids and intron homing.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Existing studies of complete fungal mitochondrial genomes reveal a potential complication to PCRbased surveys of CO1 sequence diversity, i.e., the prevalence of mobile introns (21). Therefore, we assessed the incidence and sizes of introns in the barcode region of CO1 from different fungal lineages, based on data in GenBank, to see whether their presence might lengthen target amplicons beyond what is easily recoverable by conventional PCR.…”

mentioning

confidence: 99%

Prospects for fungus identification usingCO1DNA barcodes, withPenicilliumas a test case

Seifert

Samson

deWaard

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

329

266

View full text Add to dashboard Cite

DNA barcoding systems employ a short, standardized gene region to identify species. A 648-bp segment of mitochondrial cytochrome c oxidase 1 (CO1) is the core barcode region for animals, but its utility has not been tested in fungi. This study began with an examination of patterns of sequence divergences in this gene region for 38 fungal taxa with full CO1 sequences. Because these results suggested that CO1 could be effective in species recognition, we designed primers for a 545-bp fragment of CO1 and generated sequences for multiple strains from 58 species of Penicillium subgenus Penicillium and 12 allied species. Despite the frequent literature reports of introns in fungal mitochondrial genomes, we detected introns in only 2 of 370 Penicillium strains. Representatives from 38 of 58 species formed cohesive assemblages with distinct CO1 sequences, and all cases of sequence sharing involved known species complexes. CO1 sequence divergences averaged 0.06% within species, less than for internal transcribed spacer nrDNA or ␤-tubulin sequences (BenA). CO1 divergences between species averaged 5.6%, comparable to internal transcribed spacer, but less than values for BenA (14.4%). Although the latter gene delivered higher taxonomic resolution, the amplification and alignment of CO1 was simpler. The development of a barcoding system for fungi that shares a common gene target with other kingdoms would be a significant advance.␤-tubulin ͉ cytochrome c oxidase I ͉ DNA barcoding ͉ internal transcribed spacer ͉ species identification

show abstract

“…However, the fact that at least some of the donor mtDNA that is immediately adjacent to InC9 also appeared in converted strains suggests that recombination is involved in the transfer of the InC9 sequence into the mtDNA of the recipients. The pattern of movement of InC9 involving the co-transfer of nearby upstream markers, possible via a recombination dependent pathway, is what one would expect for the movement of mobile introns (Bonen and Vogel, 2001;Hamari et al, 2001Hamari et al, , 2002Lambowitz and Belfort, 1993;Lambowitz and Zimmerly, 2004;Schäfer, 2003;Sellem et al, 1996). Moran et al (1995) showed that yeast group II introns lacking reverse trascriptase activity can move based on a DNA-level recombination mechanism.…”

Section: Persistence Of the Inc9 Element Within Populationsmentioning

confidence: 83%

A 971-bp insertion in the rns gene is associated with mitochondrial hypovirulence in a strain of Cryphonectria parasitica isolated from nature

Baidyaroy

Hausner

Hafez

et al. 2011

Fungal Genetics and Biology

View full text Add to dashboard Cite

Role of mobile introns in mitochondrial genome diversity of fungi

Cited by 27 publications

References 22 publications

Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability

Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability

Prospects for fungus identification usingCO1DNA barcodes, withPenicilliumas a test case

A 971-bp insertion in the rns gene is associated with mitochondrial hypovirulence in a strain of Cryphonectria parasitica isolated from nature

Contact Info

Product

Resources

About