Role of mixed-function amine oxidase in N-hydroxylation of 2-acetamidofluorene by hamster liver microsomal preparations

Abstract

Pretreatment of hamsters with 3-methylcholanthrene (100mg/kg body wt.) 24h before death did not appreciably change the extent of N-oxide formation when hepatic microsomal preparations were incubated with NN-dimethylaniline as substrate. In contrast, the N-hydroxylation of 2-acetamidofluorene was increased severalfold in hepatic microsomal preparations from pretreated animals. Under these conditions there were no appreciable changes in cytochrome P-450 content and NADPH-cytochrome c reductase activity. On the basis of these comparative data, it is suggested that amine oxidase is not involved in N-hydroxylation of 2-acetamidofluorene.

Determination of the N-hydroxy and phenolic metabolites of drugs derived from acetanilide by O-alkylation and selected ion monitoring

“…However, quantitation of ZV-hydroxylated compounds such as ZV-hydroxy-p-methoxyacetanilide has been difficult because of their chemical instability and the low levels produced. Methods that have been used are colorimetry (10), paper chromatography with UV detection (11), thin-layer chromatography (TLC) in combination with radioisotopically labeled compounds (12,13), GC using trimethylsilyl derivatives (14,15), and HPLC (16,17). All of these assays have certain limitations.…”