Role of mitochondrial outer membrane permeabilization during bacterial infection

Kontchou, Collins Waguia; Häcker, Georg

doi:10.1016/bs.ircmb.2022.10.002

Cited by 3 publications

(1 citation statement)

References 198 publications

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“…Virus infection activates Caspase-3, leading to cell apoptosis [20]. Caspases, a series of enzymes in the cysteine protease family, plays a pivotal function in the maintaining of homeostasis and regulating of programmed cell death in vivo.…”

Section: Discussionmentioning

confidence: 99%

Pseudorabies virus causes splenic injury via inducing the oxidative stress and apoptpsos related factors in mice

Sun,

Liu,

Yan

et al. 2023

Preprint

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Pseudorabies virus (PRV) is an immunosuppressive disease that causes significant damage to the pig industry. This study aimed to detect the effects of PRV on oxidative stress related factors and cell apoptosis in the spleen, providing a basis for the research on the pathogenesis of PRV in mice model. Pathological observation was performed by hematoxylin and eosin Y staining. Biochemical and Flow cytometry method were performed to determine the reactive oxygen species profile of the spleen post-infection and apoptosis detection. In addition, q-PCR and Western blot were adopted to measure the apoptotic conditions of the spleen infected with PRV. The results indicated that the ROS level in the PRV infection group was remarkedly increased (p<0 01) at a time-dependent pattern. Furthermore, the Malondialdehyde levels in the spleen of mice in the infection group increased significantly (p<0.01) in a time-dependent mode. However, the Catalase, Superoxide dismutase, and Glutathione activity and expression levels in the infection group were significantly decreased with the control group (p<0 01) in a time-dependent manner. Furthermore, the ratio of splenocyte apoptosis in the infection group significantly increased (p<0 05,p<0 01) in a time-dependent manner. In conclusion, PRV infection causes apoptosis of the spleen via oxidative stress in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Pseudorabies virus causes splenic injury via inducing the oxidative stress and apoptpsos related factors in mice

Sun,

Liu,

Yan

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Pseudorabies virus causes splenic injury via inducing oxidative stress and apoptosis related factors in mice

Sun,

Liu,

Yan

et al. 2023

Sci Rep

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Pseudorabies virus (PRV) is an immunosuppressive virus that causes significant damage to the pig industry. This study aimed to investigate the effects of PRV on oxidative stress and apoptotic related in the spleen of mice to provide basis knowledge for further research on the pathogenesis of PRV in mice model. 36 mice were randomly two groups, the control group which only received 200 μL PBS and infection group which was subcutaneously infected with 200 μL of 1 × 103 TCID50/100 μL PRV, respectively. Spleen tissues in each group were collected for further experiments at 48, 72, and 96 h post-infection (hpi). Pathological observation was performed by hematoxylin and eosin Y staining. Biochemical and Flow cytometry methods were used to determine the reactive oxygen species profile and apoptosis of the spleen post-infection and apoptosis detection. In addition, q-PCR and Western blot were adopted to measure the apoptotic conditions of the spleen infected with PRV. The results indicated that the reactive oxygen species (ROS) level in the PRV infection group was remarkedly increased (p < 0.01) at a time-dependent pattern. Furthermore, the Malondialdehyde levels in the spleen of mice in the infection group increased (p < 0.01) in a time-dependent mode. However, the activity of Catalase, Superoxide dismutase, and glutathione peroxidase and the content of Glutathione in the infection group were decreased with the control group (p < 0.01) at a time-dependent manner. In addition, the ratio of splenocyte apoptosis in the infection group significantly increased (p < 0.01) in a time-dependent manner. In conclusion, PRV infection causes apoptosis of the spleen via oxidative stress in mice.

show abstract

Pseudorabies virus causes splenic injury via inducing the oxidative stress and apoptpsos related factors in mice

Sun,

Liu,

Wang

et al. 2023

Preprint

View full text Add to dashboard Cite

Pseudorabies virus (PRV) is an immunosuppressive disease that causes significant damage to the pig industry. This study aimed to detect the effects of PRV on oxidative stress related factors and cell apoptosis in the spleen, providing a basis for the research on the pathogenesis of PRV in mice model. Pathological observation was performed by hematoxylin and eosin Y staining. Biochemical and Flow cytometry method were performed to determine the reactive oxygen species profile of the spleen post-infection and apoptosis detection. In addition, q-PCR and Western blot were adopted to measure the apoptotic conditions of the spleen infected with PRV. The results indicated that the ROS level in the PRV infection group was remarkedly increased (p < 0 01) at a time-dependent pattern. Furthermore, the Malondialdehyde levels in the spleen of mice in the infection group increased significantly (p < 0.01) in a time-dependent mode. However, the Catalase, Superoxide dismutase, and Glutathione activity and expression levels in the infection group were significantly decreased with the control group (p < 0 01) in a time-dependent manner. Furthermore, the ratio of splenocyte apoptosis in the infection group significantly increased (p < 0 05, p < 0 01) in a time-dependent manner. In conclusion, PRV infection causes apoptosis of the spleen via oxidative stress in mice.

show abstract

Role of mitochondrial outer membrane permeabilization during bacterial infection

Cited by 3 publications

References 198 publications

Pseudorabies virus causes splenic injury via inducing the oxidative stress and apoptpsos related factors in mice

Pseudorabies virus causes splenic injury via inducing the oxidative stress and apoptpsos related factors in mice

Pseudorabies virus causes splenic injury via inducing oxidative stress and apoptosis related factors in mice

Pseudorabies virus causes splenic injury via inducing the oxidative stress and apoptpsos related factors in mice

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