Role of miR‑520b in non‑small cell lung cancer

Zhang, Linlin; Yu, Shuangquan

doi:10.3892/etm.2018.6732

Cited by 9 publications

(3 citation statements)

References 42 publications

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“…The authors' previous study reported that Rab22a is the target gene of microRNAs (miRNAs/miR)-193b in Bc (4). Studies of miRNAs have reported that miR-520b (5), miR-204 (6) and miR-203 (7) are downregulated in Bc or other types of tumors. In addition, one of the potential targeting…”

Section: Introductionmentioning

confidence: 99%

Rab22a is a novel prognostic marker for cell progression in breast cancer

Shen

Jiang

et al. 2020

Int J Mol Med

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Breast cancer (Bc) is the most common female malignant tumor worldwide. The mechanism of tumorigenesis is still unclear. Ras-related proteins in brain (Rab)22a belongs to the Ras superfamily, which may act as an oncogene and participate in carcinogenesis. The present study aims to identify whether Rab22a could be a novel biomarker of prognosis and determine the effects of Rab22a on Bc cell progression. A total 258 BC and 56 para-tumor or non-tumor formalin fixed paraffin embedded tissues were stained through immunohistochemistry. The association between Rab22a expression and clinicopathological features, as well as overall survival status were analyzed. The expression level of Rab22a in breast cell lines were detected using reverse transcription-quantitative PcR and western blotting. SK-BR-3 cells were infected with Rab22a short hairpin RNA lenti-virus and the ability of cell proliferation, migration and invasion were measured. Gene Set Enrichment Analysis (GSEA) was employed to analyze the pathways involved in the Rab22a mRNA high level group. Rab22a was found to be overexpressed in BC tissues and upregulated in BC cells. High expression of Rab22a was related to a poor prognosis of patients with Bc. Knockdown of Rab22a decreased the proliferation, migration and invasion ability of Bc cells. GSEA indicated that certain pathways, including mammalian target of rapamycin complex 1 and protein secretion were upregulated, while pathways, such as hypoxia and KRas were downregulated in the Rab22a high level group. Rab22a is of prognostic value for Bc and necessary for Bc cell proliferation.

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Section: Introductionmentioning

confidence: 99%

Rab22a is a novel prognostic marker for cell progression in breast cancer

Shen

Jiang

et al. 2020

Int J Mol Med

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“…It was also shown that miR-NA-520b plays a role as a tumour suppressor gene in the development of NSCLC. Its downregulated expression in lung cancer tissue was negatively correlated with tumour development, lymph node metastasis, and TNM staging [59].…”

Section: Mirnas In the Pathogenesis And Progression Of Non-small Cellmentioning

confidence: 96%

MicroRNAs – novel biomarkers for malignant pleural effusions

Wojczakowski

Kobylarek

Lindner

et al. 2019

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Lung cancer is one of the most common causes of cancer death. Its poor prognosis can be attributed to the patients' advanced or metastatic presentation at the time of diagnosis. To improve and accelerate the diagnosis, better therapeutic and diagnostic methods are constantly being sought. MicroRNAs (miRNAs) are short nucleotide sequences of single-stranded, non-coding RNA that function as critical post-transcriptional regulators of gene expression. They are identified not only intracellularly, but also in physiological and pathological body fluids. These molecules are responsible for the regulation of approximately 33% of human genes, either regulating the expression of both oncogenes and suppressor genes or acting directly as an oncogene or suppressor gene itself. MiRNAs can contribute to the formation of cancer. The high specificity and sensitivity of miRNAs have been demonstrated with various malignant diseases, and for this reason, they raise particular interest as new and perspective biomarkers of tumours. Our work summarises the available information from recent years regarding the possibility of using miRNAs as biomarkers in the diagnosis of neoplasms. In this review, we focused on malignant pleural effusions with an emphasis on non-small cell lung cancer (NSCLC).

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“…Most of the studies reported that miR-520b worked as a tumor suppressor in cancer. For example, it is reported that miR-520b inhibited proliferation of lung cancer, prostate cancer, glioma, head-neck cancer and colorectal cancer et al [7,8,[19][20][21][22]. However, recently, the oncogenic role of miR-520b was also reported in ovarian cancer [9], which reveals that the effect of miR-520b may be contextdependent and needs further investigation in different tissues.…”

Section: Plos Onementioning

confidence: 99%

MLK3 is a newly identified microRNA-520b target that regulates liver cancer cell migration

Zhang

Zhu

et al. 2020

PLoS ONE

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The roles of microRNAs (miRNAs) in liver cancer have attracted much attention in recent years. In this study, we demonstrate that miR-520b is downregulated in MHCC-97H cells, a liver cancer cell line with high potential of metastasis, compared with MHCC-97L cells which has a low potential of metastasis. Furthermore, the enhanced expression of miR-520b could inhibit liver cancer cell migration, while silencing its expression resulted in increased migration. Mixed lineage kinase 3 (MLK3) was identified as a direct and functional new target of miR-520b. This regulation was also confirmed by luciferase reporter assays. In addition, our results showed that overexpression of the MLK3 expression partially reversed the effect of miR-520b on liver cancer cell migration, indicating that MLK3 contributes to the migration in liver cancer. The newly identified miR-520b/MLK3 axis partially elucidates the molecular mechanism of liver cancer cell migration and represents a new potential therapeutic target for liver cancer treatment.

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Role of miR‑520b in non‑small cell lung cancer

Cited by 9 publications

References 42 publications

Rab22a is a novel prognostic marker for cell progression in breast cancer

Rab22a is a novel prognostic marker for cell progression in breast cancer

MicroRNAs – novel biomarkers for malignant pleural effusions

MLK3 is a newly identified microRNA-520b target that regulates liver cancer cell migration

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