Role of miR‐486‐5p in regulating renal cell carcinoma cell proliferation and apoptosis via TGF‐β–activated kinase 1

He, Yanfa; Liu, Jianzhen; Wang, Yongjun; Zhu, Xiaoli; Fan, Zhengchao; Li, Chongbin; Yin, Hang; Li, Ying

doi:10.1002/jcb.26900

Cited by 27 publications

(21 citation statements)

References 32 publications

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“…For instance, miR-486-5p can regulate the production and potentiate the growth of normal red blood cells, and it can mediate the drug response of chronic myeloid leukemia progenitor cells (Wang et al, 2015). Whereas in renal cell carcinoma, miR-486-5p is decreased by interacted with the CCL2 (C-C motif chemokine ligand 2) of tumor-related macrophages, and miR-486-5p restoration is capable of inhibiting cell proliferation and inducing cell apoptosis (He et al, 2019). However, the role of miR-486-5p in LUAD remains poorly studied.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Exosome-Derived miR-486-5p Regulates Cell Cycle, Proliferation and Metastasis in Lung Adenocarcinoma via Targeting NEK2

et al. 2020

Front. Bioeng. Biotechnol.

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Objective: This study aimed to describe the mechanism of exosome-derived miR-486-5p underlying the cell cycle and progression in lung adenocarcinoma (LUAD). Methods: Bioinformatics methods were applied for identifying the differentially expressed genes (DEGs) in the GEO-LUAD dataset, predicting where the potential target miRNA was expressed and exploring the corresponding downstream target mRNA. qRT-PCR was conducted to detect the levels of the target genes in cancer cells. Thereafter, a series of in vitro experiments were performed for cell activities evaluation, including CCK-8, EdU, colony formation assay and transwell. Besides, Western blot was applied to determine the protein levels of the migration and invasion-related factors (NEK2, E-cadherin, N-cadherin, Vimentin, MMP-2, and MMP-9). Dual-luciferase reporter gene assay was employed for validating the targeted relationship between the target genes. Furthermore, nude mouse transplantation tumor experiment was conducted to further validate the role of the target miRNA in tumor development, and immunohistochemistry was used for Ki67 detection and TUNEL was applied for cell apoptosis assay. Results: miR-486-5p was observed to be enriched in serum exosomes, and seen to be significantly down-regulated in cancer tissues as well as in cancer serum exosomes. It was proven that exosomes could release miR-486-5p, thus regulating LUAD progression and affecting cell cycle. Moreover, NEK2 was identified as a target of miR-486-5p both in vivo and in vitro. Enrichment analysis revealed that NEK2 was mainly activated in cell cycle and mitosis-related pathways. Meanwhile, NEK2 was found to present significant difference in different TNM stages. Furthermore, rescue experiments indicated that the inhibitory effect of miR-486-5p overexpression on LUAD progression could be abrogated when miR-486-5p and NEK2 were simultaneously up-regulated. Conclusion: Exosome-derived miR-486-5p is responsible for cell cycle arrest as well as the inhibition of cell proliferation and metastasis in LUAD via targeting NEK2.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Exosome-Derived miR-486-5p Regulates Cell Cycle, Proliferation and Metastasis in Lung Adenocarcinoma via Targeting NEK2

et al. 2020

Front. Bioeng. Biotechnol.

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“…Furthermore, a CLDN10-associated ceRNA network was constructed in the present study, and the results revealed that miR-486-5p and miR-3919 may be the key regulators of CLDN10 expression in OC. Notably, miR-486 is reported to regulate cancer metastasis in hepatocellular carcinoma by targeting CLDN10, and miR-486-5p could inhibit cell proliferation and apoptosis in renal cell carcinoma via TGF-β-activated kinase 1 (44,45). Further investigations on the associations among CLDN10, miR-486-5p, miR-3919, TGF-β, WNT/β-catenin and EMT should be conducted in future studies, and could provide valuable insight on the cellular mechanisms underlying OC progression.…”

Section: Discussionmentioning

confidence: 99%

Claudin 10 acts as a novel biomarker for the prognosis of patients with ovarian cancer

Xuan

Huang

et al. 2020

Oncol Lett

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Ovarian cancer (OC) is one of the most fatal gynecological malignancies in the world and confers a poor 5-year survival rate. The present study was designed to discover novel prognostic markers for patients with OC in order to estimate disease metastasis or recurrence. Based on the large cohorts of transcriptome data from multicenter sources, a comprehensive analysis was performed to explore potential prognostic markers. A total of 269 differentially expressed genes were identified, of which 32 were upregulated and 237 downregulated in OC tissues compared with the corresponding expression in normal tissues. Kaplan-Meier analysis, log-rank test and nomogram analysis were employed to demonstrate that low expression levels of claudin 10 (CLDN10) were associated with a less favorable disease prognosis. The most promising prognostic marker for OC was subsequently selected. Additionally, the prognostic nomogram was constructed in order to assess the 5-year survival rate using CLDN10 expression as a prognostic marker for OC. Furthermore, gene set enrichment analysis and analysis of the tumor-associated competing endogenous RNA network were performed to elucidate the potential biological processes associated with CLDN10 expression. The current results indicated that CLDN10 may influence OC progression via transforming growth factor-β (TGF-β)-or WNT/β-catenin-induced epithelial-to-mesenchymal transition (EMT). The associations among CLDN10, microRNA-486-5p, TGF-β, WNT/β-catenin and EMT should be further investigated in future studies.

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“…MiR‐486‐5p has been featured with multiple functions in different human cancers. It has been reported that miR‐486‐5p serves as a tumor suppressor in colorectal cancer, thyroid cancer, renal cell carcinoma, and non‐small cell lung cancer . However, it has also been reported that miR‐486‐5p functions as a tumor promoter in alveolar rhabdomyosarcoma and cervical cancer .…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA DLGAP1‐AS1 promotes cell proliferation in hepatocellular carcinoma via sequestering miR‐486‐5p

Peng

Wei

2019

J of Cellular Biochemistry

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Hepatocellular carcinoma (HCC) is most prevalent tumor in liver and one of the most fatal cancers in the world. Long noncoding RNAs (lncRNAs) have been accepted as important regulators in carcinomas. But there are still many lncRNAs including DLGAP1‐AS1 unannotated in HCC. First of all, GEPIA suggested that DLGAP1‐AS1 presented high expression in HCC tissue samples relative to the normal tissues. Besides, overexpression of DLGAP1‐AS1 was also proved in HCC cell lines. Moreover, DLGAP1‐AS1 knockdown efficiently suppressed cell proliferation in HCC. Interestingly, miR‐486‐5p was predicted and validated to interact with DLGAP1‐AS1, while the level of miR‐486‐5p was significantly increased In HCC after DLGAP1‐AS1 knockdown. Moreover, we uncovered that ectopic expression of miR‐486‐5p induced suppression on HCC cell proliferation and that miR‐486‐5p inhibition offset the effect of DLGAP1‐AS1 silence on HCC cell proliferation and apoptosis. Furthermore, H3F3B was identified as target of miR‐486‐5p and was therefore positively regulated by DLGAP1‐AS1 in HCC. Of note, H3F3B upregulation partly revived the declined cell proliferative capacity in response to DLGAP1‐AS1 knockdown. To conclude, DLGAP1‐AS1 exerted its oncogenic role in HCC via miR‐486‐5p/H3F3B axis. Our new findings provided novel theoretical basis for discovery of therapeutic targets of HCC.

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Role of miR‐486‐5p in regulating renal cell carcinoma cell proliferation and apoptosis via TGF‐β–activated kinase 1

Cited by 27 publications

References 32 publications

RETRACTED: Exosome-Derived miR-486-5p Regulates Cell Cycle, Proliferation and Metastasis in Lung Adenocarcinoma via Targeting NEK2

RETRACTED: Exosome-Derived miR-486-5p Regulates Cell Cycle, Proliferation and Metastasis in Lung Adenocarcinoma via Targeting NEK2

Claudin 10 acts as a novel biomarker for the prognosis of patients with ovarian cancer

Long noncoding RNA DLGAP1‐AS1 promotes cell proliferation in hepatocellular carcinoma via sequestering miR‐486‐5p

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