Role of miR-300-3p in Leydig cell function and differentiation: A therapeutic target for obesity-related testosterone deficiency

Liang, Jinlian; Chen, Derong; Xiao, Ziyan; Wei, Siying; Liu, Yuan; Wang, Chengzhi; Wang, Zhaoyang; Feng, Yuqing; Lei, Yaling; Hu, Mei; Deng, Jingxian; Wang, Yuxin; Zhang, Qihao; Yan, Yang

doi:10.1016/j.omtn.2023.03.016

Cited by 2 publications

(2 citation statements)

References 56 publications

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“…SCs were isolated as we described previously from 3-week-old male Kunming mice [32,33]. In brief, the testes were aseptically isolated from mice, and washed 3 times in phosphate-buffered saline (PBS).…”

Section: Ex Vivo Culture Of Mouse Scsmentioning

confidence: 99%

Inhibition of miR-143-3p Restores Blood–Testis Barrier Function and Ameliorates Sertoli Cell Senescence

Xiao,

Liang,

Huang

et al. 2024

Cells

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Due to the increasing trend of delayed childbirth, the age-related decline in male reproductive function has become a widely recognized issue. Sertoli cells (SCs) play a vital role in creating the necessary microenvironment for spermatogenesis in the testis. However, the mechanism underlying Sertoli cell aging is still unclear. In this study, senescent Sertoli cells showed a substantial upregulation of miR-143-3p expression. miR-143-3p was found to limit Sertoli cell proliferation, promote cellular senescence, and cause blood–testis barrier (BTB) dysfunction by targeting ubiquitin-conjugating enzyme E2 E3 (UBE2E3). Additionally, the TGF-β receptor inhibitor SB431542 showed potential in alleviating age-related BTB dysfunction, rescuing testicular atrophy, and reversing the reduction in germ cell numbers by negatively regulating miR-143-3p. These findings clarified the regulatory pathways underlying Sertoli cell senescence and suggested a promising therapeutic approach to restore BTB function, alleviate Sertoli cell senescence, and improve reproductive outcomes for individuals facing fertility challenges.

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Section: Ex Vivo Culture Of Mouse Scsmentioning

confidence: 99%

Inhibition of miR-143-3p Restores Blood–Testis Barrier Function and Ameliorates Sertoli Cell Senescence

Xiao,

Liang,

Huang

et al. 2024

Cells

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“…In addition to their expression in germ cells, several miRNAs have been identified in Sertoli and Leydig cells, where they regulate the activity of these somatic cells [ 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ].…”

Section: The Role Of Mirnas In Spermatogenesismentioning

confidence: 99%

The Role of microRNA in Spermatogenesis: Is There a Place for Fertility Preservation Innovation?

Klees,

Alexandri,

Demeestere

et al. 2023

IJMS

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Oncological treatments have dramatically improved over the last decade, and as a result, survival rates for cancer patients have also improved. Quality of life, including concerns about fertility, has become a major focus for both oncologists and patients. While oncologic treatments are often highly effective at suppressing neoplastic growth, they are frequently associated with severe gonadotoxicity, leading to infertility. For male patients, the therapeutic option to preserve fertility is semen cryopreservation. In prepubertal patients, immature testicular tissue can be sampled and stored to allow post-cure transplantation of the tissue, immature germ cells, or in vitro spermatogenesis. However, experimental techniques have not yet been proven effective for restoring sperm production for these patients. MicroRNAs (miRNAs) have emerged as promising molecular markers and therapeutic tools in various diseases. These small regulatory RNAs possess the unique characteristic of having multiple gene targets. MiRNA-based therapeutics can, therefore, be used to modulate the expression of different genes involved in signaling pathways dysregulated by changes in the physiological environment (disease, temperature, ex vivo culture, pharmacological agents). This review discusses the possible role of miRNA as an innovative treatment option in male fertility preservation–restoration strategies and describes the diverse applications where these new therapeutic tools could serve as fertility protection agents.

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Role of miR-300-3p in Leydig cell function and differentiation: A therapeutic target for obesity-related testosterone deficiency

Cited by 2 publications

References 56 publications

Inhibition of miR-143-3p Restores Blood–Testis Barrier Function and Ameliorates Sertoli Cell Senescence

Inhibition of miR-143-3p Restores Blood–Testis Barrier Function and Ameliorates Sertoli Cell Senescence

The Role of microRNA in Spermatogenesis: Is There a Place for Fertility Preservation Innovation?

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