Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy

Wang, Jiawei; Xiao, Ying; Zhang, Han

doi:10.1167/iovs.16-20559

Cited by 9 publications

(9 citation statements)

References 6 publications

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“…Recently, we showed that expression of miR-146a was significantly higher in GO than non-GO orbital adipose tissues 2. In a previous study, we focused on the role played by miR-146a in the regulation of inflammation in GO and suggested that miR-146a may positively affect the anti-inflammatory process 2. Likewise, miR-146a has been shown to be upregulated in many autoimmune diseases and plays a key role in negative feedback.…”

Section: Discussionmentioning

confidence: 99%

“…Transfection of orbital fibroblasts with miR-146a mimics, inhibitors and control was performed according to the respective manufacturer’s protocol 2. The miR-146a mimics and inhibitors were purchased from Ambion/Applied Biosystems (MC10722; Ambion, Austin, Texas, USA) and Exiqon (4103092–001: Exiqon, Vedbaek, Denmark), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…qRT-PCR and western blotting were performed as described previously 2. Briefly, for qRT-PCR, total RNA was extracted from orbital fibroblasts according to manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…They can participate in a wide variety of biological processes including human diseases such as cancer,13–15 inflammatory disease,2 16–19 obesity,19 infections20 21 and oxidative stress-associated disorders 22 23. Recently, we showed that the level of microRNA-146a (miR-146a) expression was significantly higher in GO orbital adipose tissue than in that of non-GO 2. In addition, we found that miR-146a was upregulated by inflammatory stress in orbital fibroblasts and reported that miR-146a may play a role in the pathogenesis of GO.…”

Section: Introductionmentioning

confidence: 99%

“…Although the pathogenesis of GO remains unclear, the main pathomechanism involves three mechanisms: (1) inflammation,2 (2) adipogenesis3–5 and (3) glycosaminoglycan (GAG) accumulation. In GO, GAG accumulates in extraocular muscles and orbital connective tissues 6.…”

Section: Introductionmentioning

confidence: 99%

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Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves’ orbitopathy

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…qRT-PCR and western blotting were performed as described previously 2. Briefly, for qRT-PCR, total RNA was extracted from orbital fibroblasts according to manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves’ orbitopathy

et al. 2017

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Expression and polymorphism of micro-RNA according to body mass index and breast cancer presentation in Tunisian patients

Belaiba

Medimegh

Ammar

et al. 2018

Journal of Leukocyte Biology

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Micro-RNAs (miRs) constitute a class of small noncoding RNAs implicated in the regulation of gene expression by binding to target mRNAs. A miR can target several mRNAs, being involved in different biologic processes and pathologies. This pleiotropic function might explain the link between diseases co-occurrence. Epigenetic origin of the link between obesity and breast cancer (BC) is investigated in a cohort of Tunisian patients, focusing on polymorphism at germline level (miR-146a) and on expression in mammary tumors (miR-21, miR-146a, and miR-34a), according to body mass index (BMI) and clinico-pathologic features. The measure of miR expression in 60 mammary tumors was realized using quantitative RT-PCR. Study of rs 2910164 in miR-146a was performed by PCR and direct sequencing using blood DNA of 83 affected women and 50 unrelated subjects from Great Tunis. MiR-21, miR-146a, and miR-34a have been quantified in breast tumor according to BMI. MiR-21 is significantly more expressed in tumors of obese women comparatively to nonobese patients. On the contrary, miR-34a is decreased in tumors of obese women. Moreover, in obese BC patients, a significant increase in both miR-21 and miR-146a expression is revealed in cases with lymph node metastasis. The polymorphism at rs 2910164 (miR-146a) locus was not shown as a risk factor for BC. However the mutant CC genotype was revealed to be associated with a risk for bad outcome of the disease. Chronic inflammation in obese women would be linked to aggressive breast tumors via induction of oncomiRs overexpression and decrease of tumor suppressor miRs. K E Y W O R D Sbody mass index, mammary tumors, miR-146a, miR-21, miR-34a tions, transcription of these miRs is at baseline levels. In the context of inflammation, several stimuli such as cytokines (TNF-, IL-1 ) or ligands of TLR such LPS are capable of regulating the expression of these

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miR-762 modulates thyroxine-induced cardiomyocyte hypertrophy by inhibiting Beclin-1

et al. 2019

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Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy

Cited by 9 publications

References 6 publications

Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves’ orbitopathy

Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves’ orbitopathy

Expression and polymorphism of micro-RNA according to body mass index and breast cancer presentation in Tunisian patients

miR-762 modulates thyroxine-induced cardiomyocyte hypertrophy by inhibiting Beclin-1

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