Role of mineralizing cartilage in osteoclast and osteoblast recruitment

Wijngaert, Frank P. van de; Schipper, C.A.; Tas, M.C.; Burgér, E.H.

doi:10.1016/8756-3282(88)90107-x

Cited by 22 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No fusion with detached osteoclasts was observed, even though over 300 cells were examined, nor were mononucleate cells in close proximity to detached osteoclasts. The close proximity of mineralized bone or cartilage matrix is believed to be necessary for recruitment, differentiation (Krukowski and Kahn, 1982;van de Wijngaert et al, 1988), and multinucleation of osteoclasts (Glowacki and Lian, 1987). Therefore, fusion resulting in multinucleation probably occurs only after cells have attached to the bone surface.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the functional stages of osteoclasts by enzyme histochemistry and electron microscopy

1991

View full text Add to dashboard Cite

To investigate the functional stages of osteoclasts, the ultrastructural histochemical distribution of the lysosomal enzymes [acid phosphatase (tartrate-sensitive) and neutral phosphatase], the plasma membrane enzymes [alkaline phosphatase, Ca(++)-ATPase, and alkaline ouabain-insensitive p-nitrophenylphosphatase (alkaline p-NPPase)], and the mitochondrial enzyme (cytochrome C oxidase) was evaluated in the chicken tibial metaphysis. Both active-appearing and detached (resting) osteoclasts were studied. Serial sectioning was used to identify detached osteoclasts which were present in the perivascular space. The ultrastructure of detached osteoclasts was similar to that of active osteoclasts, except for the lack of a ruffled border and clear zone, and an altered distribution pattern of small vesicles. Small vesicles were uniformly distributed in the cytoplasm of resting osteoclasts, whereas they were concentrated beneath the ruffled border of active osteoclasts. Alkaline p-NPPase, a marker enzyme for the basal ruffled border, was also apparent on the membrane of small vesicles. However, the vesicles did not possess Ca(++)-ATPase, a marker enzyme for the apical plasma membrane. These findings support the concept that small vesicles serve as a membrane reservoir for the ruffled border membrane. Pre-osteoclasts contained abundant mitochondria and lysosomes, prominent Golgi complexes, moderately developed endoplasmic reticulum, and lacked small vesicles. Pre-osteoclasts appear to fuse with osteoclasts which are attached to the bone surface, but not with detached osteoclasts. The small vesicles, from which the ruffled border arises, are absent from pre-osteoclasts, suggesting that they develop after fusion with pre-existing osteoclasts or after attachment to the bone surface. Alkaline p-NPPase appears to be a marker for differentiation of pre-osteoclasts to mature osteoclasts.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the functional stages of osteoclasts by enzyme histochemistry and electron microscopy

1991

View full text Add to dashboard Cite

show abstract

“…The generation of TRAP ϩ cells in E18 and E19 in the absence of MMPs is somewhat smaller (Blavier and Delaissé, 1995;this work). This may be due to the modulating effect that resorption may exert on the recruitment of new osteoclasts (Van De Wijngaert et al, 1988), or to decreased VEGF bioavailability which may participate in osteoclast differentiation (Niida et al, 1999).…”

Section: Mmp-9 Is a Major Mmp For Osteoclast Recruitment In Primitive Long Bones And Is Specifically Required For The Passage Of The Cellmentioning

confidence: 99%

Matrix Metalloproteinase 9 and Vascular Endothelial Growth Factor Are Essential for Osteoclast Recruitment into Developing Long Bones

Engsig

Chen

et al. 2000

The Journal of Cell Biology

533

415

View full text Add to dashboard Cite

Bone development requires the recruitment of osteoclast precursors from surrounding mesenchyme, thereby allowing the key events of bone growth such as marrow cavity formation, capillary invasion, and matrix remodeling. We demonstrate that mice deficient in gelatinase B/matrix metalloproteinase (MMP)-9 exhibit a delay in osteoclast recruitment. Histological analysis and specialized invasion and bone resorption models show that MMP-9 is specifically required for the invasion of osteoclasts and endothelial cells into the discontinuously mineralized hypertrophic cartilage that fills the core of the diaphysis. However, MMPs other than MMP-9 are required for the passage of the cells through unmineralized type I collagen of the nascent bone collar, and play a role in resorption of mineralized matrix. MMP-9 stimulates the solubilization of unmineralized cartilage by MMP-13, a collagenase highly expressed in hypertrophic cartilage before osteoclast invasion. Hypertrophic cartilage also expresses vascular endothelial growth factor (VEGF), which binds to extracellular matrix and is made bioavailable by MMP-9 (Bergers, G., R. Brekken, G. McMahon, T.H. Vu, T. Itoh, K. Tamaki, K. Tanzawa, P. Thorpe, S. Itohara, Z. Werb, and D. Hanahan. 2000. Nat. Cell Biol. 2:737–744). We show that VEGF is a chemoattractant for osteoclasts. Moreover, invasion of osteoclasts into the hypertrophic cartilage requires VEGF because it is inhibited by blocking VEGF function. These observations identify specific actions of MMP-9 and VEGF that are critical for early bone development.

show abstract

“…al. [13] observed that osteoclasts were recruited when periost-free live or devitalized fetal bone rudiments were transplanted under the renal capsule of adult syngenic mice. The results of EDX-analysis in the present study showed both hypo-mineralization in alveolar bone and normal mineralization in dentin.…”

Section: Discussionmentioning

confidence: 96%

Disturbance of the eruption of the first molar and ankylosis of tooth root by HEBP administration in newly born rat

Hanzawa¹,

Kindaichi

et al. 1994

J Bone Miner Metab

View full text Add to dashboard Cite

A bstract:In this study, we have investigated the effects of HEBP on alveolar bone remodeling and molar tooth development of new born rats especially with regard to the relation of the mineralization rate and tooth eruption. New born rats (3 to 5 days after birth) were injected with 10 mg/kg body weight HEBP subcutaneously every day except Sunday. Mandibles were investigated radiologically and histologically. The mineralization rate of alveolar bone and dentin was assessed by energy-dispersive Xray (EDX) analyis. Soft X-ray radiography disclosed the failure of the first molar tooth eruption, and histological findings showed ankylosis of alveolar bone and tooth root, and dentin resorption by multinucleated giant cells. The result of EDX analysis revealed hypo-mineralization of alveolar bone and normal mineralization of dentin. These data suggest that preosteoclasts might be recruited at the site of alveolar bone and tooth germ interface but could not differentiate into bone-resorbing mature osteoclasts because of hypo-mineralization of alveolar bone.

show abstract

Role of mineralizing cartilage in osteoclast and osteoblast recruitment

Cited by 22 publications

References 21 publications

Characterization of the functional stages of osteoclasts by enzyme histochemistry and electron microscopy

Characterization of the functional stages of osteoclasts by enzyme histochemistry and electron microscopy

Matrix Metalloproteinase 9 and Vascular Endothelial Growth Factor Are Essential for Osteoclast Recruitment into Developing Long Bones

Disturbance of the eruption of the first molar and ankylosis of tooth root by HEBP administration in newly born rat

Contact Info

Product

Resources

About