Antimicrobial resistance (AMR) data on zoonotic and indicator bacteria from humans, animals and food are collected annually by the EU Member States (MSs) and reporting countries, jointly analysed by EFSA and ECDC and presented in a yearly EU Summary Report. This report provides an overview of the main findings of the 2020–2021 harmonised AMR monitoring in
Salmonella
spp.,
Campylobacter jejuni
and
C. coli
in humans and food‐producing animals (broilers, laying hens and turkeys, fattening pigs and bovines under 1 year of age) and relevant meat thereof. For animals and meat thereof, indicator
E. coli
data on the occurrence of AMR and presumptive Extended spectrum β‐lactamases (ESBL)‐/AmpC β‐lactamases (AmpC)‐/carbapenemases (CP)‐producers, as well as the occurrence of methicillin‐resistant
Staphylococcus aureus
are also analysed. In 2021, MSs submitted for the first time AMR data on
E. coli
isolates from meat sampled at border control posts. Where available, monitoring data from humans, food‐producing animals and meat thereof were combined and compared at the EU level, with emphasis on multidrug resistance, complete susceptibility and combined resistance patterns to selected and critically important antimicrobials, as well as
Salmonella
and
E. coli
isolates exhibiting ESBL‐/AmpC‐/carbapenemase phenotypes. Resistance was frequently found to commonly used antimicrobials in
Salmonella
spp. and
Campylobacter
isolates from humans and animals. Combined resistance to critically important antimicrobials was mainly observed at low levels except in some
Salmonella
serotypes and in
C. coli
in some countries. The reporting of a number of CP‐producing
E. coli
isolates (harbouring
bla
OXA‐48
,
bla
OXA‐181
, and
bla
NDM‐5
genes) in pigs, bovines and meat thereof by a limited number of MSs (4) in 2021, requests a thorough follow‐up. The temporal trend analyses in both key outcome indicators (rate of complete susceptibility and prevalence of ESBL‐/AmpC‐ producers) showed that encouraging progress have been registered in reducing AMR in food‐producing animals in several EU MSs over the last years.