Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma

Mortoglou, Maria; Djordjević, Aleksandra Buha; Djordjević, Vladimir; Collins, Hunter; York, Lauren; Mani, Katherine; Valle, Elizabeth Ranier Martins do; Wallace, David R.; Uysal-Onganer, Pinar

doi:10.1007/s00204-021-03196-9

Cited by 7 publications

(12 citation statements)

References 143 publications

(195 reference statements)

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“…Each of the five cell lines was plated as it has been previously described [ 60 ]. Cells were incubated for 48 h with increasing concentrations of the Ni compounds or vehicle (12 concentrations, 0–1 mM).…”

Section: Methodsmentioning

confidence: 99%

“…The measurement of β-catenin was determined using a commercially available kit for measuring human β-catenin in cell lysates (MyBioSource, Inc. #MBS266009; San Diego, CA, USA) and used as it has described in our previously published data based on the manufacturer’s protocol [ 60 ]. β-catenin was quantified by measuring the absorbance at 450 nm and extrapolating to the standard curve.…”

Section: Methodsmentioning

confidence: 99%

“…Exposure was initiated by the addition of 50 μM NiCl 2 , Ni-acetate or vehicle for 48 h in a 37 °C/5% CO 2 incubator. After exposure, caspase 3/7 activity was determined as it has described previously [ 60 ]. Emitted fluorescence was measured using a Bio-Tek ® plate reader and Gen5 software at 485 ex /530 em .…”

Section: Methodsmentioning

confidence: 99%

“…RNU6-snRNA was used as reference RNA to normalise miR expression levels, forward, 5′-GCTTCGGCAGCACATATACTAAAAT-3′ and reverse 5′-CGCTTCACGAATTTGCGTGTCAT-3. The conditions for thermocycling were as described previously [ 60 ]. The miR-155, miR-126, and miR-221 expression levels were normalised to RNU6 using the 2^ ΔΔCT method [ 61 ].…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies have shown that environmental toxins, such as Cd, can promote their toxicity through miRs [ 12 , 18 , 60 ]. Toxic metals such as Ni have not been studied as extensively as Cd, and, therefore, only a small number of miRs associated with Ni exposure has been characterized.…”

Section: Introductionmentioning

confidence: 99%

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Nickel’s Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs

Mortoglou

Manić

Djordjevic

et al. 2022

Toxics

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Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancer types with a limited overall survival rate due to the asymptomatic progression of symptoms in metastatic stages of the malignancy and the lack of an early reliable diagnostic biomarker. MicroRNAs (miRs/miRNAs) are small (~18–24 nucleotides), endogenous, non-coding RNAs, which are closely linked to the development of numerous malignancies comprising PDAC. Recent studies have described the role of environmental pollutants such as nickel (Ni) in PDAC, but the mechanisms of Ni-mediated toxicity in cancer are still not completely understood. Specifically, Ni has been found to alter the expression and function of miRs in several malignancies, leading to changes in target gene expression. In this study, we found that levels of Ni were significantly higher in cancerous tissue, thus implicating Ni in pancreatic carcinogenesis. Hence, in vitro studies followed by using both normal and pancreatic tumor cell lines and increasing Ni concentration increased lethality. Comparing LC50 values, Ni-acetate groups demonstrated lower values needed than in NiCl2 groups, suggesting greater Ni-acetate. Panc-10.05 cell line appeared the most sensitive to Ni compounds. Exposure to Ni-acetate resulted in an increased phospho-AKT, and decreased FOXO1 expression in Panc-10.05 cells, while NiCl2 also increased PTEN expression in Panc-10.05 cells. Specifically, following NiCl2 exposure to PDAC cells, the expression levels of miR-221 and miR-155 were significantly upregulated, while the expression levels of miR-126 were significantly decreased. Hence, our study has suggested pilot insights to indicate that the environmental pollutant Ni plays an important role in the progression of PDAC by promoting an association between miRs and Ni exposure during PDAC pathogenesis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nickel’s Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs

Mortoglou

Manić

Djordjevic

et al. 2022

Toxics

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Mechanisms of Heavy Metal Cadmium (Cd)-Induced Malignancy

Wang,

Gan,

Tang

2024

Biol Trace Elem Res

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Toxic mechanisms of cadmium and exposure as a risk factor for oral and gastrointestinal carcinomas

Tavakoli Pirzaman,

Ebrahimi,

Niknezhad

et al. 2023

Hum Exp Toxicol

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Incidence and mortality rates of gastrointestinal (GI) and oral cancers are among the highest in the world, compared to other cancers. GI cancers include esophageal, gastric, colon, rectal, liver, and pancreatic cancers, with colorectal cancer being the most common. Oral cancer, which is included in the head and neck cancers category, is one of the most important causes of death in India. Cadmium (Cd) is a toxic element affecting humans and the environment, which has both natural and anthropogenic sources. Generally, water, soil, air, and food supplies are reported as some sources of Cd. It accumulates in organs, particularly in the kidneys and liver. Exposure to cadmium is associated with different types of health risks such as kidney dysfunction, cardiovascular disease, reproductive dysfunction, diabetes, cerebral infarction, and neurotoxic effects (Parkinson’s disease (PD) and Alzheimer’s disease (AD)). Exposure to Cd is also associated with various cancers, including lung, kidney, liver, stomach, hematopoietic system, gynecologic and breast cancer. In the present study, we have provided and summarized the association of Cd exposure with oral and GI cancers.

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Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma

Cited by 7 publications

References 143 publications

Nickel’s Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs

Nickel’s Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs

Mechanisms of Heavy Metal Cadmium (Cd)-Induced Malignancy

Toxic mechanisms of cadmium and exposure as a risk factor for oral and gastrointestinal carcinomas

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